Purification and breeding method of spirulina species

Abstract

The invention provides a purification and breeding method of a spirulina species. The method comprises the following steps: (1) preparing 3L of a spirulina species culture solution; (2) filtering the culture solution; (3) transferring a spirulina gel, which is obtained from filtration, into a triangular flask, adding 200-300ml of medium L6, conducting adaptive restoration culture, and controlling environments as follows: illumination is 4000lx and temperature is 35 DEG C; (4) conducting alkaline cleaning on the spirulina gel; (5) cleaning the spirulina gel; (6) reserving supernatant spirulina of liquid, adding 50-100ml of medium L6, and uniformly mixing the materials; (7) diluting an obtained mixture; (8) transferring 1ml of the liquid to a sterilized 1-5ml centrifuge tube, and conducting adaptive restoration culture on the centrifuge tube in a water bath of 2000lx and 30 DEG C for 12h; and (9) selecting large individual spirulina having a relatively high amount of spirals by virtue of a single-filament separator, and conducting enlarge cultivation on the large individual spirulina having a relatively high amount of spirals so as to achieve the large-scale production of the spirulina species. With the application of the method, by effectively conducting multi-stage enlarge cultivation on single-filament algae, pure species free from miscellaneous bacteria or miscellaneous algae can be obtained.

Description

technical field

[0001] The invention relates to a method for purifying and breeding spirulina species, in particular to a method for purifying and breeding spirulina species that meets the needs of purebred cultivation and research. Background technique

[0002] Spirulina has been widely used in food, medicine, health products, cosmetics and other industries. Purification of spirulina without bacteria or algae is a difficult task for many spirulina researchers or enterprises. In the field of scientific research, pure Spirulina and breeding methods mainly include capillary pipette microscope separation method and plate coating separation method. Due to the limitations of the method itself, the controllability and difficulty of manipulation are poor, resulting in low efficiency and high efficiency in obtaining pure Spirulina. Chances of purebreds are also low. In terms of enterprise application, for decades, Spirulina algae species have been continuously passed on and used a...