Purification and breeding method of spirulina species

A technology of spirulina and algae species, applied in the direction of bacteria, etc., can solve the problems of low efficiency of pure breeding, difficulty in adapting, poor controllability, etc.

Inactive Publication Date: 2017-04-19
杭州元点生物科技有限公司
View PDF2 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing technology of the same kind invented the separation method of monofilament algae through capillary pipette micro-separation method, which has poor controllability and difficulty in operation, low efficiency and low probability of obtaining pure species, and it is difficult t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] A method for purifying and breeding spirulina species, comprising the following steps: (1) taking 3 L of spirulina algae culture fluid; (2) filtering the culture fluid; (3) filtering the filtered algae Transfer the mud into the triangular flask, add 200-300ml of medium L6, and carry out adaptive recovery culture. The control environment is light 4000lx and temperature 35°C; (4) Alkaline cleaning of the algae mud; (5) Cleaning of the algae mud ; (6) Take the upper layer of the liquid to suspend the algae, add 50-100ml medium L 6 , and mix well; (7) Dilute; (8) Take 1ml of liquid, transfer it into a 1-5ml sterilized centrifuge tube, place the centrifuge tube in a 2000lx 30°C water bath, and carry out adaptive recovery culture for 12 hours; ( 9) In the sterile clean bench, use a monofilament separator to select a single spirulina with large algal body and more spirals, and then expand the single spirulina with large algal body and more spirals to produce algal species in b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a purification and breeding method of a spirulina species. The method comprises the following steps: (1) preparing 3L of a spirulina species culture solution; (2) filtering the culture solution; (3) transferring a spirulina gel, which is obtained from filtration, into a triangular flask, adding 200-300ml of medium L6, conducting adaptive restoration culture, and controlling environments as follows: illumination is 4000lx and temperature is 35 DEG C; (4) conducting alkaline cleaning on the spirulina gel; (5) cleaning the spirulina gel; (6) reserving supernatant spirulina of liquid, adding 50-100ml of medium L6, and uniformly mixing the materials; (7) diluting an obtained mixture; (8) transferring 1ml of the liquid to a sterilized 1-5ml centrifuge tube, and conducting adaptive restoration culture on the centrifuge tube in a water bath of 2000lx and 30 DEG C for 12h; and (9) selecting large individual spirulina having a relatively high amount of spirals by virtue of a single-filament separator, and conducting enlarge cultivation on the large individual spirulina having a relatively high amount of spirals so as to achieve the large-scale production of the spirulina species. With the application of the method, by effectively conducting multi-stage enlarge cultivation on single-filament algae, pure species free from miscellaneous bacteria or miscellaneous algae can be obtained.

Description

technical field [0001] The invention relates to a method for purifying and breeding spirulina species, in particular to a method for purifying and breeding spirulina species that meets the needs of purebred cultivation and research. Background technique [0002] Spirulina has been widely used in food, medicine, health products, cosmetics and other industries. Purification of spirulina without bacteria or algae is a difficult task for many spirulina researchers or enterprises. In the field of scientific research, pure Spirulina and breeding methods mainly include capillary pipette microscope separation method and plate coating separation method. Due to the limitations of the method itself, the controllability and difficulty of manipulation are poor, resulting in low efficiency and high efficiency in obtaining pure Spirulina. Chances of purebreds are also low. In terms of enterprise application, for decades, Spirulina algae species have been continuously passed on and used a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20
CPCC12N1/20
Inventor 李雷吴华勇刘松民韦根源
Owner 杭州元点生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap