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Porcine compound synbiotics micro-ecological preparation and preparation method thereof

A technology of microecological preparations and synbiotics, applied in the field of microecological preparations, can solve problems such as drug residues and reduce the quality of pork products, and achieve the effects of stimulating growth, reducing the impact of pork quality, and maintaining intestinal health

Inactive Publication Date: 2017-04-19
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a compound synbiotic microecological preparation for pigs and its preparation method, aiming to solve the problems caused by the abuse of antibiotics leading to more and more pathogenic bacteria developing drug resistance, drug residues, reducing the quality of pork products and the accumulation of antibiotics in the food chain. Issues that have a serious impact on human health

Method used

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  • Porcine compound synbiotics micro-ecological preparation and preparation method thereof

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preparation example Construction

[0029] Such as figure 1Shown: the preparation method of the composite synbiotic probiotics for pigs specifically includes:

[0030] S101: Lactobacillus, Bifidobacterium, Bacillus and Saccharomyces probiotics are isolated and screened from the soil in the pig farm, the feed trough for feeding pigs, and the gastrointestinal tract area, and the biological characteristics of the isolated probiotics are carried out , identification of function and safety, and evaluation of probiotic effects in vitro, preliminary screening of probiotics;

[0031] S102: Utilize the relationship between pig intestinal microbes, sugar metabolism and vitamins to screen out high-quality probiotics that can improve energy acquisition and storage, and improve the ability of pigs to obtain energy from diets, and use high-quality probiotics in pig intestines Improve energy acquisition and storage, improve the ability of pigs to obtain energy from diets, and then screen high-quality pig intestinal probiotics...

Embodiment 1

[0046] Example 1: Preliminary screening of yeast probiotics from the soil in a pig farm.

[0047] The first step: the collection of soil samples containing yeast. Select the soil of the adult pig breeding place in a large farm as the collection destination, randomly select 3-5 small areas, take 20-40g of original soil from each area, divide it into 2 groups, put them into sterilized ziplock bags, and bring them back quickly Store in a 4°C refrigerator in the laboratory for later use;

[0048] The second step: the separation of yeast. Under sterile conditions, the two groups of soil samples were added to the enriched culture solution to form colonies, and then the bacteria were inoculated into the bacterial suspension made of sterile normal saline, and then diluted into different strains by the 10-serial decreasing method. Inoculate the samples of different solubility gradients into the culture medium by streaking on the plate, make 3 parallels for each dilution, and put the ...

Embodiment 2

[0052] Preliminary screening of Bacillus subtilis from pig feed grains.

[0053] The first step: the collection of samples in the feed trough. Select the breeding places of adult pigs in various large-scale slaughterhouses as the sample collection destination, randomly select several feed troughs, take 20-30g samples from each place, put them into sterilized ziplock bags, and bring them back to the laboratory and place them in a 4°C refrigerator Save it for later use;

[0054] The second step: enrichment and preliminary isolation of Bacillus subtilis. Under aseptic conditions, the two groups of feed tank samples were added to the enriched culture solution to form colonies, and then the bacteria were inoculated into sterile normal saline to make a bacterial suspension, and then diluted by 10 serial decrement method to obtain For different concentration gradients, inoculate samples of different solubility gradients onto the culture medium by streaking on the plate, make 3 para...

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Abstract

The invention discloses a porcine compound synbiotics micro-ecological preparation and a preparation method thereof. The micro-ecological preparation consists of probiotics and prebiotics, wherein the probiotics and the prebiotics are mixed at a ratio of 1 to (1-5); the probiotics include lactobacillus, bifidobacterium, bacillus and saccharomycetes; the prebiotics include oligosaccharide and an acidifying agent; the oligosaccharide includes mannose-oligosaccharide, fructooligosaccharide and isomaltooligosaccharide; and the acidifying agent is is a compound acidifying agent which is composed of lactic acid, citric acid and fumaric acid. The micro-ecological preparation provided by the invention can overcome weaknesses of probiotic products which are independently used for breeding commercial pigs and the like, and the micro-ecological preparation is applicable to commercial pigs of various types; the micro-ecological preparation is capable of enhancing advantages and avoiding disadvantages, and promoting the planting of exogenous probiotics in animal intestinal tracts; in addition, various probiotics can be selectively stimulated to rapidly grow or reproduce in pig bodies, so that the purpose of promoting the health of the commercial pigs is achieved, and the production performance of the commercial pigs is also greatly improved; and the micro-ecological preparation can relieve the influence of conventional animal edible additives to the quality of pork; therefore, the micro-ecological preparation is more economic and applicable.

Description

technical field [0001] The invention belongs to the field of microecological preparations, in particular to a composite synbiotic microecological preparation for pigs and a preparation method thereof. Background technique [0002] The emergence of probiotics is due to the improvement of people's living standards, and people gradually realize that antibiotics added to feed for a long time can cause harm to human health through the food chain. At present, there are few studies on the application of synbiotics microecological preparations in animal production in my country, and the use of microecological preparations in animal food additives in animal husbandry is still relatively small. People are vague about the application of probiotics, that is, confusion is targeted Sexually added objects, resulting in animal growth that is often less than expected. The research and application of microecological preparations overcomes the various disadvantages caused by the long-term use ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/01C12R1/225C12R1/07C12R1/645A23K50/30A23K10/18A23K20/163A23K20/105
CPCC12N1/16C12N1/20A23V2400/11A23V2400/51
Inventor 刘君刘华康王晗陈静罗雁刘永明张薇
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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