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Identification method for glyphosate-resistant maize variety

A technology for glyphosate resistance and variety identification, applied in botany equipment and methods, seed and rhizome treatment, horticulture, etc., can solve the problems of insufficient stability of identification results, limited application conditions, limited application environment, etc., and achieve identification The effect of stable and accurate results, low environmental dependence, and simple operation

Inactive Publication Date: 2017-04-26
HENAN ACAD OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This identification method has obvious defects such as large space occupation, long operation time, and limited application environment. In addition, the efficacy of glyphosate is easily affected by weather factors such as temperature, light, and precipitation during spraying. The identification results of this method are not stable enough, and the application conditions are relatively limited

Method used

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  • Identification method for glyphosate-resistant maize variety
  • Identification method for glyphosate-resistant maize variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The glyphosate-resistant corn variety identification method provided by the application specifically includes the following steps:

[0042] (1) Soak the corn seeds to be tested in a clear aqueous solution with a glyphosate mass concentration of 0.1-2.0%, and soak for 8-24 hours at room temperature (18-25°C);

[0043] At the same time set water control;

[0044] (2) Cultivate the corn seeds soaked in step (1) at 25-28°C, 16 h light / 8 h dark;

[0045] During cultivation, corn seeds are placed in a nutrient bowl for cultivation, and in the nutrient bowl, nutrient soil and vermiculite are mixed in a ratio of 1:1;

[0046] (3) Perform statistics on morphological and physiological indicators such as emergence rate of corn seeds in step (2), fresh weight of whole seedlings, root length, stem length, leaf weight and leaf chlorophyll content,

[0047]And compared with the clean water control group, if there is no significant change in each index, it can be considered that the ...

Embodiment 2

[0065] Based on the experimental results of Example 1, the glyphosate-resistant corn variety identification method of the present application can play a good role in the purity identification of glyphosate-resistant corn. The relevant experimental detection process is briefly introduced as follows.

[0066] sample setup

[0067] The samples included 4 kinds: the sample to be tested (glyphosate-resistant corn CC-2 and its genetic background parent Zheng 58, mixed according to the ratio of seed number 3:1, randomly sampled), Z58E-1, inbred lines Zheng 58 and Hybrid Zhengdan 1002;

[0068] Process group settings

[0069] Divided into 4 treatment groups:

[0070] Treatment 1, sow in the field, wait until the corn grows to the 4-6 leaf stage, spray the Roundup water agent with a final concentration of glyphosate of 0.5%;

[0071] Treat 2, after soaking seeds with 0.5% concentration glyphosate, sow in nutrient bowl;

[0072] Process 3, after soaking seeds with 0.5% concentration...

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Abstract

The invention belongs to the technical field of agriculture variety identification, and particularly relates to an identification method for a glyphosate-resistant maize variety. The method includes the steps that to-be-identified maize seeds are arranged in a clean water solution with the glyphosate mass concentration being 0.1%-2.0% to be soaked, a clean water contrast is arranged, the seeds are cultivated, morphological index and physiological index statistics is conducted, and comparison and identification are conducted. The identification method can be used for detecting the function efficiency of a target glyphosate-resistant gene, and can play a good role on the aspects of glyphosate-resistant crop parent purity identification, hybrid seed production purity identification and the like. The identification method for the glyphosate-resistant maize variety is an indoor identification method, the method is easy to operate, the operation time is short, the dependency on the environment is low, the identification result is stable and accurate, and the identification method has good application value for glyphosate-resistant maize variety identification, variety screening and cultivation.

Description

technical field [0001] The application belongs to the technical field of agricultural variety identification, and specifically relates to a patent application for a glyphosate-resistant corn variety identification method. Background technique [0002] In recent years, the annual planting area of ​​corn in China has exceeded 550 million mu, making it the largest food crop in my country. Weeds are one of the major hazards in maize production, causing losses of about 10% of the total yield each year. Artificial weeding has been basically replaced by chemical weeding because it cannot meet the needs of modern agricultural production. Compared with other herbicides, glyphosate has the characteristics of low price, high efficiency, broad spectrum, low toxicity, and no residue, and the resistant weeds are kept at a relatively low level all year round. Therefore, it has ranked the world's pesticide sales for many years first place. [0003] Glyphosate is a non-selective herbicide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C1/00
CPCA01C1/00A01G22/00
Inventor 燕树锋铁双贵刘海芳孙书库齐建双岳润清卢彩霞韩小花郭书磊
Owner HENAN ACAD OF AGRI SCI