A kind of capillary network and its preparation method

A capillary and vascular endothelium technology, which is applied in the field of capillary network and its preparation, can solve the problems of inability to accurately obtain two-dimensional and three-dimensional micro-nano structures of artificial blood vessels, and inability to directly print micro-nano blood vessels, so as to overcome important obstacles. , the effect of reducing energy consumption costs and improving safety

Active Publication Date: 2019-05-10
叶川 +5
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even though the artificial blood vessel prepared by this method has a bionic inner and outer surface microstructure and good biocompatibility, the two-dimensional and three-dimensional micro-nano structure of the artificial blood vessel cannot be accurately obtained.
Even one of the most viable technologies in the current rapid prototyping industry—three dimensional printing (3DP) technology cannot directly print micro-nano-scale blood vessels.

Method used

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  • A kind of capillary network and its preparation method
  • A kind of capillary network and its preparation method

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preparation example Construction

[0038] The present invention provides a method for preparing a capillary network. A soluble three-dimensional spinning net 4 is prepared by a centrifugal spinning method, and animal cells 9 are cultivated on the obtained soluble three-dimensional spinning net 4. The silk screen 4 grows and proliferates, and after the soluble three-dimensional spinning screen 4 is completely dissolved, a capillary network is obtained.

[0039] In view of the many problems existing in the prior art, the present invention uses a specific method to prepare a capillary network by means of centrifugal spinning. Centrifugal spinning is a high-efficiency and low-consumption micro-nanofiber preparation technology, and its principle is that polymer melt Or the solution is stretched into fibers under the action of centrifugal force after being thrown out from the spinneret hole. In this process, the centrifugal force plays an important role, and it has a certain compaction effect on the polymer melt or s...

Embodiment 1

[0070] A method for preparing a capillary network, comprising the steps of:

[0071] 1) Melt 50g of Tanghuatang and sterilize it under high pressure, then store it in a solution state for later use;

[0072] 2) Dissolve 5g of medical-grade polyvinyl alcohol in 50mL of ultrapure water to prepare a solution with a concentration of 10% by mass, and let it stand at 4°C after sterilization; then add vascular endothelial growth factor and alkaline The fibroblast growth factor is fully mixed with the resulting solution;

[0073] 3) Pour the solution obtained in step 1 and step 2 into a sterile high-speed rotating coaxial centrifugal spinning machine (sterilized) with two liquid storage cylinders inside and outside, respectively, to carry out coaxial centrifugal spinning; coaxial spinning The process conditions are: the inner cylinder solution is the shaft core material, the outer cylinder solution is the shaft shell material, the centrifugal speed is 3000r / min, the coaxial nozzle di...

Embodiment 2

[0075] A method for preparing a capillary network, comprising the steps of:

[0076] 1) Melt 50g of white granulated sugar and sterilize it under high pressure, then save it in solution state for later use;

[0077]2) Dissolve 5g of medical-grade polyvinyl alcohol and 2.5g of medical-grade polyethylene glycol in 50mL of ultrapure water, sterilize the resulting solution and let stand at 4°C; then mix vascular endothelial growth factor and alkaline The fibroblast growth factor is fully mixed with the resulting solution;

[0078] 3) Pour the solution obtained in step 1 and step 2 into a sterile high-speed rotating coaxial centrifugal spinning machine (sterilized) with two liquid storage cylinders inside and outside, respectively, to carry out coaxial centrifugal spinning; coaxial spinning The process conditions are: the inner cylinder solution is the shaft core material, the outer cylinder solution is the shaft shell material, the centrifugal speed is 4500r / min, the coaxial nozz...

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Abstract

The invention provides a blood capillary network and a making method thereof. A soluble three-dimensional spinning network is made by adopting a centrifugal spinning method, animal cells are cultured on the soluble three-dimensional spinning network and grow and proliferate along the soluble three-dimensional spinning network, and the blood capillary network is obtained after the soluble three-dimensional spinning network is completely dissolved. A high-voltage electrostatic field and a large number of molds are not needed, a centrifugal force is only utilized as the power for producing the soluble three-dimensional spinning network, the production yield is greatly improved, the energy consumption cost is greatly reduced, the safety of production operation is improved, the demand for large-scale production of the three-dimensional spinning network of engineering-oriented tissues is met, and the important clinical obstacle of tissue engineering structures are also overcome. The blood capillary network is low in preparation cost, and required three-dimensional blood capillary networks can be provided for establishment of full and effective blood capillary network systems of thick engineering-oriented tissues or organs such as the liver and kidney.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a capillary network and a preparation method thereof. Background technique [0002] Although some tissue-engineered organs such as skin, trachea and other tissues that do not require high blood supply have begun to be used clinically, and satisfactory results have been achieved. However, it is not so optimistic to achieve transplant survival in vivo for thicker solid organs such as liver and kidney. Therefore, it is necessary to construct a sufficient and effective capillary network system. [0003] At present, related technologies for constructing artificial blood vessels in vitro include dipping-leaching method, coagulation method, decellularized tissue composite method, coaxial electrospinning method, rotational exposure method, and 3D rapid prototyping technology. The decellularized tissue composite method is to cut the small intestinal submucosa membrane into a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/54A61L27/50A61L27/38A61L27/16D01D5/18
CPCA61L27/16A61L27/3804A61L27/3808A61L27/507A61L27/54A61L2300/414D01D5/18C08L29/04
Inventor 叶川邹强杨龙李靖孙宇马敏先
Owner 叶川
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