A kind of preparation method of Phellinus granule strain

A technology of Phellinus granules, applied in the field of preparation of Phellinus granules, can solve the problems affecting the speed of colonization and feeding effect, slow transfer of cultivated species, growth inhibition, etc., to achieve the vitality and success rate of the strains Improve and facilitate the presence or absence of bacterial contamination and the effect of more germination points

Active Publication Date: 2019-07-23
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of strain production method usually has low inoculation efficiency, long growth cycle, and lumps after being covered with hyphae. It is not easy to mash it when using it. The speed of colonization and the effect of feeding are often affected by the damage of the mycelia, and the contamination of bacteria, hypoxia and growth inhibition of the mycelium due to poor sealing or too little ventilation during the cultivation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of Phellinus liquid strain:

[0035] Aseptically inoculate the mother species of Phellinus Phellinus into the liquid strain culture medium for shaking culture, the culture conditions are: temperature 26°C, shaker speed 150r / min, and shake culture for 5 days in the dark to obtain Phellinus liquid strains; Liquid medium composition: corn flour 40g / L, glucose 25g / L, yeast extract 10g / L, bran 20g / L, KH 2 PO 4 1.25g / L, MgSO 4 ·7H 2 O 0.625g / L, the balance of deionized water.

Embodiment 2

[0037] Preparation of fungal elicitors:

[0038] (1) Soak 10g of strain mycelium in 200-400mL of 70% ethanol for 24h, centrifuge at 5000r / min for 20-30min, and collect the residue;

[0039] (2) Add 100-200mL of distilled water to the residue collected in step (1), extract at 100°C for 1-2h, centrifuge at 5000r / min for 20-30min, and collect the supernatant and residue respectively;

[0040] (3) Repeat step (2) for the residue collected in step (2) once;

[0041] (4) Combine the supernatant collected in steps (2) and (3), add 800-1600mL of 95% ethanol for 24 hours, centrifuge at 5000r / min for 30min, and collect the precipitate; the obtained precipitate is the fungal elicitor;

[0042] Described bacterial strain is T-type Heterobasidiomycetes or thin perennial bacteria 。 The fungal elicitors prepared from the strain of Poremus perinaetum are referred to as P elicitors; the fungal elicitors prepared from the strain of Heterobasidioides T-type are referred to as H elicitors.

Embodiment 3

[0044] Preparation of Phellinus granules:

[0045] (1) Selection of materials: select millet with husk as the main material of the granule strain;

[0046] (2) Material pretreatment: Weigh 98kg of husk millet and soak in tap water for 12 hours to ensure sufficient water absorption;

[0047] (3) Cooking in boiling water: transfer the soaked millet into the pot with sufficient water absorption, add water to cover 3cm of the material surface, gradually heat until the water boils, the millet is cooked but not rotten, and when there is no white heart, it can be taken out Drain;

[0048] (4) Mixing material: Add 1 kg of gypsum powder, 1 kg of sucrose and 1.5 g of the H exciter prepared in Example 2 to the drained millet, and stir evenly;

[0049] (5) Bottling: choose a 240mL tissue culture bottle, fill the mixed material to two-thirds of the total volume, and then screw on the bottle cap with a vent filter;

[0050] (6) Sterilization: Transfer the tissue culture bottle after loadin...

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Abstract

The invention relates to a preparation method of a phellinus igniarius granular spawn, belonging to the technical field of edible fungi cultivation. The preparation method takes a mixture of gypsum powder, sucrose, a fungal elicitor and ripened cereals as a culture medium; the preparation method is characterized in that the cereals with shell or skin are taken as main materials of the granular spawn culture medium, and the fungal elicitor is added into the main materials. The cereals with shell or skin are taken as the main materials of the granular spawn, so that the granular spawn is comprehensive in nutrition, can meet the growth demand of phellinus igniarius mycelia, is strong in spawn activity, is more in germination points after being inoculated, and can shorten the culture time. Compared with a preparation method without adding the fungal elicitor, the preparation method provided by the invention can obtain spawn extracellular enzyme having obviously improved activity under the premise that other conditions are the same (the cultivation time is the same), so that the activity of the obtained spawn is improved.

Description

technical field [0001] The invention relates to a method for preparing Phellinus granule strains, and belongs to the technical field of edible fungus cultivation. Background technique [0002] Phellinus Sanghuang porus sanghuang It is one of the most famous medicinal fungi in my country. It is obligate to parasitize on Morus trees. It belongs to Basidiomycota taxonomically, Agaricomycetes, Hymenochaetales, Hymenochaetaceae, Morus Yellow Sanghuang porus . According to the records of "Medicinal Properties": Phellinus japonica is slightly bitter in taste and cold in nature. It is used in traditional Chinese medicine to treat dysentery, night sweats, metrorrhagia, bloody stranguria, astringent pain in the navel and abdomen, prolapse of the anus, diarrhea, and amenorrhea; "Shen Nong's Materia Medica" Phellinus is described as "long-term use to lighten the body and prolong life", and it also has the functions of detoxification and improving the function of the digestive system....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/38C12N11/02A01G18/40C12R1/645
CPCA01G18/00C12N1/14C12N1/38C12N11/02
Inventor 田雪梅张国利王光远赵晨
Owner QINGDAO AGRI UNIV
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