Fermentation culture medium, fungus chaff fermentation bacterium agent and fermentation method

A technology of fermentation medium and fungus chaff, which is applied in the field of microbial resource application, can solve the problems of low utilization rate of fungus chaff and low enzyme activity, and achieve the effects of simple operation, easy implementation, and shortened degradation time

Active Publication Date: 2017-05-10
武汉迪奥药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The first object of the present invention is to provide a kind of fermentation medium, to alleviate the technical problem that the enzyme activity that produces when white rot fungus and Aspergillus niger are cultivated in the prior art is relatively low;
[0010] The third object of the present invention is to provide a method for fermenting bacterial chaff to alleviate the technical problem of low utilization of bacterial chaff in the prior art

Method used

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  • Fermentation culture medium, fungus chaff fermentation bacterium agent and fermentation method
  • Fermentation culture medium, fungus chaff fermentation bacterium agent and fermentation method
  • Fermentation culture medium, fungus chaff fermentation bacterium agent and fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A fermentation medium, consisting of the following components by weight:

[0047] 6 parts of dextrin, 0.5 parts of bean cake powder, 3 parts of yeast powder, Na 2 PO 4 0.4 parts, KCl 0.2 parts, MgSO 4 ·7H 2 O 0.05 parts, FeSO 4 ·7H 2 O 0.05 parts, NH 4 0.3 parts of Cl, CaCO 3 0.5 parts, 8 parts of vitamin nutrient solution, 6 parts of grain hydrolyzate, and 75 parts of water.

[0048] Among them, the vitamin nutrient solution consists of inositol 30g, vitamin B 1 1g, vitamin B 6 1g, 2g folic acid, 0.05g biotin dissolved in 1000mL distilled water. The grain hydrolyzate is composed of buckwheat noodles, water and hydrochloric acid in a mass ratio of 5:25:1, and is hydrolyzed for 75 minutes at 65°C and 0.06MPa pressure.

[0049] A bacterium chaff fermented bacterial agent, the live bacteria density is 1×10 6 CFU / mL of white rot fungus was added to the fermentation medium provided in this example, 150r / min, 30°C for 24h, and then added to the fermentation med...

Embodiment 2

[0052] A fermentation medium, consisting of the following components by weight:

[0053] 3 parts of dextrin, 0.1 part of bean cake powder, 1 part of yeast powder, Na 2 PO 4 0.1 part, KCl 0.1 part, MgSO 4 ·7H 2 O 0.01 part, FeSO 4 ·7H 2 O 0.01 parts, NH 4 Cl 0.1 part, CaCO 3 0.2 parts, 5 parts of vitamin nutrient solution, 1 part of grain hydrolyzate, 60 parts of water.

[0054] Among them, the vitamin nutrient solution consists of inositol 25g, vitamin B 1 0.5g, vitamin B 6 0.5g, folic acid 1g, biotin 0.01g dissolved in 1000mL distilled water. The grain hydrolyzate is composed of buckwheat noodles, water and hydrochloric acid in a mass ratio of 5:25:1, and is hydrolyzed for 60 minutes at 65°C and 0.05MPa pressure.

[0055] A bacterium chaff fermented bacterial agent, the live bacteria density is 5×10 5 CFU / mL white rot fungus was added to the fermentation medium provided in this example, 150r / min, 30°C for 24h, and then added to the fermentation medium with a de...

Embodiment 3

[0058] A fermentation medium, consisting of the following components by weight:

[0059] 8 parts of dextrin, 1 part of bean cake powder, 5 parts of yeast powder, Na 2 PO 4 0.7 parts, KCl 0.4 parts, MgSO 4 ·7H 2 O 0.1 part, FeSO 4 ·7H 2 O 0.1 parts, NH 4 0.5 parts of Cl, CaCO 3 0.8 parts, 10 parts of vitamin nutrient solution, 10 parts of grain hydrolyzate, 90 parts of water.

[0060] Among them, the vitamin nutrient solution consists of inositol 35g, vitamin B 1 2g, vitamin B 6 2g, folic acid 3g, biotin 0.1g dissolved in 1000mL distilled water. The grain hydrolyzate is composed of buckwheat noodles, water and hydrochloric acid in a mass ratio of 5:25:1, and is hydrolyzed for 90 minutes at 65°C and 0.08MPa pressure.

[0061] A bacterium chaff fermented bacterial agent, the live bacteria density is 5×10 6 CFU / mL white rot fungus was added to the fermentation medium provided in this example, 150r / min, 30°C for 24h, and then added to the fermentation medium with a d...

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Abstract

The invention provides a fermentation culture medium, a fungus chaff fermentation bacterium agent and a fermentation method and relates to the technical field of application of microorganism resources. The fermentation culture medium provided by the invention can be used for effectively stimulating the germination of spores and the growth of thalli when being used for culturing white rot fungi and aspergillus niger; the enzyme activity of a ligninolytic enzyme produced by the white rot fungi is relatively high and the enzyme activity of cellulase produced by the aspergillus niger is relatively high. According to the fungus chaff fermentation bacterium agent provided by the invention, the fermentation culture medium provided by the invention is used for culturing optimized combination of the matched white rot fungi and aspergillus niger, and cellulose and lignin long chains are easy to crack, so that microorganisms are easy to degrade, a degradation process is accelerated and the degradation time is shortened. According to the fermentation method of fungus chaff, provided by the invention, the fungus chaff is fermented by utilizing the fermentation culture medium provided by the invention; the fermentation method is simple and convenient to operate, easy to implement and economical and practical.

Description

technical field [0001] The invention relates to the technical field of the application of microbial resources, in particular to a fermentation medium, a fungus chaff fermentation agent and a fermentation method. Background technique [0002] Mushroom chaff refers to the culture medium waste produced after the cultivation of edible fungi, mainly composed of crude fiber, crude protein and crude fat. With the rapid development of the edible fungi industry, more and more fungus chaff is produced after the production of edible fungi. At present, China is the world's largest producer of shiitake mushrooms, with an annual output of 1.68×10 dried mushrooms. 7 kg. While the mushroom industry brings economic benefits, it also brings resource waste and environmental pollution. Every time 100kg of fresh mushrooms are harvested, 60kg of fungus chaff will be produced. These fungus chaffs are rich in nutrients and are easy to spoil and deteriorate. waste of resources and pollute the en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K10/12C12R1/685C12R1/645
Inventor 邢承华蒋红英方勇何英俊
Owner 武汉迪奥药业有限公司
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