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Quick and low-toxic mosquito paraffin section manufacturing method

A technology of paraffin section and production method, which is applied in the field of medical pathology, and achieves the effects of ensuring uniformity, being convenient for fixation, and reducing the time for filming

Inactive Publication Date: 2017-05-10
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: Aiming at the deficiencies in the prior art, a fast and low-toxic method for making paraffin paraffin slices of mosquitoes is simple in operation, short in time consumption, low in toxicity, and can observe the complete spatial structure of mosquitoes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A quick, low-toxic method for making mosquito paraffin sections, comprising the following steps:

[0027] 1) Artificial feeding Take several mosquitoes, starve them for 12 hours, put them into the mosquito cage, and put them into the sponge block dipped in mouse blood, take out the mosquitoes after 30 minutes.

[0028] 2) Material collection and fixation Freeze the mosquitoes after feeding, then remove the legs and wings, put them into the fixative solution Bouin's solution, fix them under negative pressure for 1 hour, continue to fix them at room temperature for 12 hours, wash them twice with 60% alcohol, Then preserve it in 60% alcohol (both refers to the concentration) for later use.

[0029] 3) Dehydration and transparency Take the fixed worm bodies and dehydrate them with ethanol solutions with concentration gradients of 60%, 70%, 90%, 100% Ⅰ, and 100% Ⅱ at 4°C. The treatment time of each gradient is for 1 hour, and finally added to the transparent agent methylcyc...

Embodiment 2

[0035] A quick, low-toxic method for making mosquito paraffin sections, comprising the following steps:

[0036] 1) Artificial feeding Take several mosquitoes, starve for 18 hours, put them into the mosquito cage, and put them into the sponge block dipped in mouse blood, take out the mosquitoes after 60 minutes.

[0037] 2) Material collection and fixation Freeze and numb the fed mosquitoes, then remove the legs and wings, put them in fixative solution for fixation, fix at negative pressure -0.1MPa for 1h, continue to fix at room temperature for 15h, wash with 60% alcohol three times, Then store in 60% alcohol for later use.

[0038] 3) Dehydration and transparency Take the fixed worm bodies and dehydrate them with ethanol solutions with concentration gradients of 60%, 70%, 90%, 100% Ⅰ, and 100% Ⅱ at 4°C. The treatment time of each gradient is For 1 hour, finally add to the transparent agent and heat to boil to make transparent. 100% Ⅰ and 100% Ⅱ are ethanol with a concentra...

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Abstract

The invention discloses a quick and low-toxic mosquito paraffin section manufacturing method and belongs to the technical field of microscopic tissue section. The method includes the steps of artificially feeding mosquitoes, preparing and fixing materials, dehydrating and transparentizing the materials, performing paraffin infiltration and embedding, cutting the materials into sections, and dyeing and sealing the sections. In the paraffin section manufacturing method, the mosquitoes are processed into the sections after being fed, so that intestines and esophagus of the mosquitoes can be observed intuitively. Before fixation, feet and wings of the mosquitoes are removed, so that on one hand, the mosquitoes are convenient to fix, and on the other hand, the follow-up paraffin infiltration process is accelerated and uniformity of the paraffin infiltration is ensured. Meanwhile, the fixing and paraffin infiltration processes in the method are carried out under negative pressure, so that infiltration of a fixing liquid and the paraffin is improved, thereby ensuring successful section cutting. In addition, the method reduces section preparation time at certain degree, and improves work efficiency. Finally, the method is free of xylene, so that the body damage on technical staffs in pathology department and environment pollution are reduced greatly.

Description

technical field [0001] The invention relates to the field of medical pathology, in particular to a fast and low-toxic method for making mosquito paraffin sections. Background technique [0002] Paraffin section method is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science. method. The basic process of the paraffin section method is to wash, dehydrate, transparent, and soak the fixed tissue for inspection first, and then embed the tissue into a wax block with paraffin, then slice and dye it with a microtome, and finally make a paraffin section. [0003] Paraffin section technique is also an important experimental technique for studying mosquito tissue morphology and histopathology. For the study of mosquito sli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/286G01N2001/2873
Inventor 马晓君
Owner WEIFANG MEDICAL UNIV
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