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Non-invasive gene mutation detection in lung cancer patients

A technology for lung cancer and detection probes, applied in the direction of microbial determination/inspection, measuring devices, instruments, etc., can solve problems such as limitations, time-consuming, and complexity

Active Publication Date: 2017-05-10
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These techniques are complex, technology-dependent, and time-consuming, and thus limited in clinical use

Method used

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  • Non-invasive gene mutation detection in lung cancer patients
  • Non-invasive gene mutation detection in lung cancer patients
  • Non-invasive gene mutation detection in lung cancer patients

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0106] Example 1 Non-invasive detection of EGFR gene mutation in lung cancer patients

[0107] The detection of EGFR mutations by the system of the present invention was developed and validated in four consecutive phases. First, an in vitro spike-in experiment was performed with a human lung cancer cell line harboring two specific human EGFR mutations: c.2236_2250del15, which encodes the p.E746-A750 mutation present in the HCC827 cell line (penetotic 19 deletion), and c.2573T>G, which encodes the p.L858R mutation in the NCI-H1975 cancer cell line. Second, mutant EGFR DNA in body fluids was detected as a function of lung tumor burden in a mouse lung cancer xenograft model. Plasma from mice xenografted with the human lung cancer cell line NCI-H1975 (carrying the EGFR L858R mutation) was tested at various stages of tumor progression. Third, the system was used to detect EGFR mutations in saliva and plasma of NSCLC patients. Finally, EGFR mutation detection was validated in s...

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PUM

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Abstract

A system and method for the detection of saliva biomarkers in bodily fluids is described. In particular, the system is suitable for detecting biomarkers of lung cancer in a subject. The system includes an electrochemical sensor chip having at least one well, wherein the at least one well contains a working electrode coated with a conducting polymer functionalized with at least one capture probe, and at least one labeled detector probe. When the at least one labeled detector probe is mixed with a sample of the subject containing a biomarker of lung cancer and added to the at least one well, an electric current is applied to the sample, such that when at least some of the biomarker binds to the capture probe, a measurable change in electric current in the sample is created that is indicative of lung cancer.

Description

[0001] Cross references to related patent applications [0002] This application claims priority to US Provisional Patent Application No. 62 / 007,286, filed June 3, 2014, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] Statement Regarding Federally Sponsored Research or Development [0004] This invention was made with Government support under TR00124 awarded by the National Institutes of Health. The government has certain rights in this invention. Background technique [0005] Lung cancer has the highest incidence of all cancers and is the leading cause of cancer-related death worldwide, accounting for 29% of all cancer deaths in men and 26% of all cancer deaths in women (Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin 2012;62:10-29). Recent understanding of lung cancer pathogenesis and molecular oncology has led to the discovery of the biological and therapeutic importance of acquired genet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/574
CPCG01N33/5438G01N33/57423G01N27/3276C12Q1/6825C12Q2537/125C12Q2565/607C12Q2600/156C12Q1/6886C12Q2600/158
Inventor 魏芳大卫·T·W·翁苏武舟
Owner RGT UNIV OF CALIFORNIA