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A microfluidic chip and mixing system for cell freezing and its control method

A microfluidic chip and mixing system technology, applied in fluid controllers, mixers, laboratory containers, etc., can solve the problems of lack of cell fine manipulation, so as to improve the survival rate, meet the needs of the industry, and stabilize the survival rate effect

Active Publication Date: 2022-05-10
SHENZHEN VITAVITRO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some industry giants have launched cell factories and freezing equipment, but the operation process of these equipment is still based on the traditional manual operation process, which does not have the possibility of fine manipulation of cells

Method used

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  • A microfluidic chip and mixing system for cell freezing and its control method
  • A microfluidic chip and mixing system for cell freezing and its control method
  • A microfluidic chip and mixing system for cell freezing and its control method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Such as Figure 1-Figure 3 As shown, a microfluidic chip for cell freezing includes a microfluidic chip body, and the microfluidic chip body includes a first reagent inflow channel 1, a second reagent inflow channel 2, and a reagent mixing flow channel. Channel 3, third reagent inflow channel 4, cell inflow channel 5, recoil channel 6, reaction chamber 7, cell mixture outflow channel 8, the first reagent inflow channel 1, the second reagent inflow channel The channel 2 communicates with the inlet of the reagent mixing channel 3 respectively, and the outlet of the reagent mixing channel 3 communicates with the reaction chamber 7. The inner wall of the reaction chamber 7 is arc-shaped, and the reaction chamber 7 and the cell mixture flow out The inner diameter of the flow channel 8 is larger than the outer diameter of the cells. The cell mixture outflow channel 8 is connected with a cell mixture storage chamber 9 , and the inner diameter of the cell mixture storage chamb...

Embodiment 2

[0051] On the basis of Example 1, such as Figure 4 As shown, the microfluidic chip for cell freezing includes a liquid storage pool 10, the liquid storage pool 10 is connected to the cell inflow channel 5 through a connecting pipe 11, and the inner diameter of the connecting pipe 11 is greater than 150 μm; The height of the reservoir 10 is higher than that of the microfluidic chip.

Embodiment 3

[0053] On the basis of Example 1, such as Figure 5 As shown, the reagent mixing channel 3 is provided with obstacles 12 for thorough mixing.

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Abstract

The invention provides a microfluidic chip for cell freezing, a mixing system and a control method thereof, wherein the microfluidic chip includes a microfluidic chip body, and the microfluidic chip body includes a first reagent inflow channel , the second reagent inflow channel, the reagent mixing channel, the third reagent inflow channel, the cell inflow channel, the recoil channel, the reaction chamber, the cell mixture outflow channel, the first reagent inflow channel, the second The second reagent inflow channel is respectively connected with the inlet of the reagent mixing channel, and the reagent mixing channel, the third reagent inflow channel, the cell inflow channel, the recoil channel, and the cell mixture outflow channel are respectively connected with the reaction chamber , the inner diameters of the reaction chamber, the cell inflow channel, and the cell mixture outflow channel are larger than the outer diameter of the cells. The technical scheme of the present invention is used to replace manual operation to realize cell processing, and cooperate with freezing equipment to realize automatic freezing processing of egg cells or other cells, with better stability and more precision.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for freezing cells, a mixing system and a control method thereof. Background technique [0002] With the development of biotechnology and the needs of biomedicine, human assisted reproduction and animal husbandry conservation and breeding. After more than 60 years of development, cell freeze-thaw technology has become a feasible method for cell and biological tissue preservation. Among them, cryopreservation of human oocytes has attracted the most attention and has the widest application prospects. The infertility rate in China has increased from 3% in 1992 to about 20% now, and the main medical way to solve infertility is test-tube baby. In 2009, there were 138 IVF institutions in China. As of 2012, there were 356 institutions and 17 sperm banks approved to carry out human assisted reproductive technology. Although the number of institutions has doubled in just three...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01F33/30G01N15/10G01K7/02
CPCG01K7/02G01N15/10B01L3/5027B01L3/50273G01N2015/1006B01L2200/0621B01L2300/0867B01L2300/0645B01L2400/0475B01F33/3017B01F33/304B01F2101/23
Inventor 林小贞盖博·瓦吉它
Owner SHENZHEN VITAVITRO BIOTECH CO LTD
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