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Application of gene knockout animal as skin barrier function disorder animal model

A technology of gene knockout and dysfunction, applied in animal husbandry and other fields, can solve the problems of poor uniformity of model preparation standards, many uncontrollable factors, and poor similarity of clinical disease models

Inactive Publication Date: 2017-05-24
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the animal models of skin mechanical barrier dysfunction produced by the acetone ether method and the adhesive tape method are cumbersome, have many uncontrollable factors, and the uniformity of the model preparation standards is poor, especially the similarity with clinical disease models.

Method used

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  • Application of gene knockout animal as skin barrier function disorder animal model
  • Application of gene knockout animal as skin barrier function disorder animal model
  • Application of gene knockout animal as skin barrier function disorder animal model

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Experimental program
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Effect test

Embodiment Construction

[0029] 1: Construction of transgenic mice

[0030] The inventors utilized the Cre / loxP recombinase system to convert Cdc42 loxp / loxp Transgenic mice were crossed with K5-Cre transgenic mice, and the genotype of the F1 generation mice obtained was Cdc42 loxp / + / -K5-Cre+. Then use Cdc42 loxp / + / -K5-Cre+ mice and Cdc42 loxp / loxp Transgenic mice were crossed, and their offspring obtained 1 / 4 ratio of Cdc42 gene knockout (knockout) mice, referred to as KO mice, and the genotype was Cdc42 loxp / loxp / -K5-Cre+; Obtain 1 / 2 ratio of Cdc42 gene non-knockout (wild type) mice, referred to as WT mice, the genotype is Cdc42 loxp / + / K5-Cre- or Cdc42 loxp / loxp / -K5-Cre-. Build process such as figure 1 shown.

[0031] Gene level and protein level identification were carried out by PCR method and Cdc42 immunohistochemical method.

[0032] Mouse tissue PCR gene identification: K5-Cre+ is a 667bp specific band, Cdc42 loxP / loxP It is a 700bp specific band, WT is a 600bp specific band; K5-...

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Abstract

The invention discloses an application of a gene knockout animal as a skin barrier function disorder animal model. A skin keratinocyte Cdc42 gene knockout mouse is formed, the skin barrier function of the mouse is found to be obviously destroyed, and in the expression spectrum level of the gene, similar to clinical skin barrier function destruction type diseases such as specific atopic dermatitis (AD) and the like, the skin keratinocyte Cdc42 gene knockout mouse can be used as the animal model of the skin barrier function destruction type disease for foundation and clinical experiment research.

Description

technical field [0001] The invention is a novel animal model, and particularly relates to the application of a genetically advanced animal as an animal model of skin barrier dysfunction. Background technique [0002] The skin is the organ most directly in contact with the outside world, and it is also the largest organ in the human body. It covers the entire body surface and plays an important role as a barrier. As an important physical / mechanical barrier of the body, on the one hand, it can prevent external adverse factors (physical, chemical, biological, etc.) from entering the human body to a certain extent, and protect various organs and tissues in the body from the invasion of external substances; , can avoid the loss of water and lipids in the epidermis and dermis of the human body, so as to maintain the relative stability of the internal environment of the body. [0003] Studies have shown that the occurrence of various skin diseases such as atopic dermatitis (AD), i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 陈英华张琳胡国栋邵龙泉徐梦影吕嘉文李以然
Owner SOUTHERN MEDICAL UNIVERSITY
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