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Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells

A dendritic cell, mature technology, applied in the field of cell biology, can solve problems such as low surface marker expression, poor activated immune function, and limited development

Inactive Publication Date: 2017-05-24
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experimental studies have shown that the maturation of DC cells induced by interferon a is insufficient, the expression of surface markers is low, and the activation of immune function is poor, which largely limits the development of clinical application of DC vaccines

Method used

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  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells
  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells
  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: culturing DCs cells

[0044] 1. Two volunteers signed the informed consent, and 30ml of peripheral venous blood was drawn respectively. After the lymphocyte separation medium (Tianjin Haoyang, TBD) separated PBMC, they were suspended in fresh serum-free dendritic cell culture medium (X-VIVO from Lonza Company), and the cell density was 5×10 5 -2×10 6 / ml, 37°C, 5% CO 2 cultured in an incubator.

[0045]2. After 4-5 days of culture, add CEA antigen to a final concentration of 5 μg / ml, load for 24 hours, add CD40L to a final concentration of 2 μg / ml, and MPLA to a final concentration of 40 ng / ml, at 37°C, 5% CO 2 Mature dendritic cells were harvested after 24 hours in the incubator. Observe the cell morphology on day 7, as figure 1 .

[0046] from figure 1 It can be seen that the cells of the two volunteers were in a semi-adherent state, with fine axons on the surface, and a few of them gathered into clusters, that is, they showed the typical morpholo...

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Abstract

The invention belongs to the field of cell biology and discloses a combination factor for promoting maturity of dendritic cells and a method for cultivating the dendritic cells. The combination factor comprises CD40L and MPLA. The combination factor is utilized to stimulate the dendritic cells, efficiently activate autologous T cells thereof and induce the dendritic cells to mature fully. According to the method for cultivating the dendritic cells, the combination factor for promoting maturity of the dendritic cells is used to stimulate immature dendritic cells. According to the method, the dendritic cells can be induced to mature fully and have the capability of efficiently activating the autologous T cells thereof, the operation method is simple, the cost is low, cultivated mature DC cells can be extensively used for preparing tumor immunotherapy medicines or vaccines to play an important role in the field of tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a combination factor for promoting maturation of dendritic cells and a method for cultivating dendritic cells. Background technique [0002] In 1973, Steinman and Cohn discovered a group of dendritic cells when they cultured mouse splenocytes in vitro, so they named them dendritic cells (DCs). Since then, Steinman found that DCs are the most powerful stimulator of mixed lymphocyte reaction (MLR) in the study of its function, and deduced that DCs are "helper" cells that activate T and B lymphocyte activation. This inference was fully confirmed in subsequent studies. It is currently believed that DCs are the professional antigen-presenting cells with the strongest antigen-presenting ability in vivo, and they are also the only cells that can activate the initial T lymphocyte response. [0003] DCs originate from bone marrow myeloid stem cells and lymphoid stem cells, and ar...

Claims

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Application Information

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IPC IPC(8): C12N5/0784
CPCC12N5/0639C12N2500/90C12N2501/52
Inventor 葛啸虎陈海佳王一飞陶然张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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