Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells

A dendritic cell, mature technology, applied in the field of cell biology, can solve problems such as low surface marker expression, poor activated immune function, and limited development

Inactive Publication Date: 2017-05-24
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experimental studies have shown that the maturation of DC cells induced by interferon a is insufficient, the expression of surface mar

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells
  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells
  • Combination factor for promoting maturity of dendritic cells and method for cultivating dendritic cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: culturing DCs cells

[0044] 1. Two volunteers signed the informed consent, and 30ml of peripheral venous blood was drawn respectively. After the lymphocyte separation medium (Tianjin Haoyang, TBD) separated PBMC, they were suspended in fresh serum-free dendritic cell culture medium (X-VIVO from Lonza Company), and the cell density was 5×10 5 -2×10 6 / ml, 37°C, 5% CO 2 cultured in an incubator.

[0045]2. After 4-5 days of culture, add CEA antigen to a final concentration of 5 μg / ml, load for 24 hours, add CD40L to a final concentration of 2 μg / ml, and MPLA to a final concentration of 40 ng / ml, at 37°C, 5% CO 2 Mature dendritic cells were harvested after 24 hours in the incubator. Observe the cell morphology on day 7, as figure 1 .

[0046] from figure 1 It can be seen that the cells of the two volunteers were in a semi-adherent state, with fine axons on the surface, and a few of them gathered into clusters, that is, they showed the typical morpholo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of cell biology and discloses a combination factor for promoting maturity of dendritic cells and a method for cultivating the dendritic cells. The combination factor comprises CD40L and MPLA. The combination factor is utilized to stimulate the dendritic cells, efficiently activate autologous T cells thereof and induce the dendritic cells to mature fully. According to the method for cultivating the dendritic cells, the combination factor for promoting maturity of the dendritic cells is used to stimulate immature dendritic cells. According to the method, the dendritic cells can be induced to mature fully and have the capability of efficiently activating the autologous T cells thereof, the operation method is simple, the cost is low, cultivated mature DC cells can be extensively used for preparing tumor immunotherapy medicines or vaccines to play an important role in the field of tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a combination factor for promoting maturation of dendritic cells and a method for cultivating dendritic cells. Background technique [0002] In 1973, Steinman and Cohn discovered a group of dendritic cells when they cultured mouse splenocytes in vitro, so they named them dendritic cells (DCs). Since then, Steinman found that DCs are the most powerful stimulator of mixed lymphocyte reaction (MLR) in the study of its function, and deduced that DCs are "helper" cells that activate T and B lymphocyte activation. This inference was fully confirmed in subsequent studies. It is currently believed that DCs are the professional antigen-presenting cells with the strongest antigen-presenting ability in vivo, and they are also the only cells that can activate the initial T lymphocyte response. [0003] DCs originate from bone marrow myeloid stem cells and lymphoid stem cells, and ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/0784
CPCC12N5/0639C12N2500/90C12N2501/52
Inventor 葛啸虎陈海佳王一飞陶然张维敏
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap