Enzyme immobilization method and application thereof

Abstract

The invention provides an enzyme immobilization method and application thereof, belonging to the field of biochemical engineering; the enzyme immobilization method is capable of being used for combining enzyme with a good carrier, which takes an organic solvent as a solvent, in an immobilized way without influencing the activity of the enzyme. The enzyme immobilization method comprises the following steps: preparing an enzyme-containing buffer solution into ice particles; dipping the ice particles into a polymer casting solution which takes the organic solvent as the solvent, and enabling the surface of the ice particles to be soaked into the polymer casting solution; taking out the ice particles, then putting the ice particles into cryogenic equipment, and using a way of directly forming a polymeric membrane on the surface of the ice particles or forming a polymeric membrane by reacting with a gradually dissolved ice surface to obtain an enzyme product coated with the polymeric membrane while the solvent in the polymer casting solution is evaporated. The enzyme immobilization method can be applied to a process of beer production.

Description

technical field [0001] The invention belongs to the field of biochemical industry, in particular to an enzyme immobilization method and application thereof. Background technique [0002] As one of the fruits of bioengineering research, enzyme preparations have been widely popularized in various industries. For example, in the beer production industry, enzyme preparations can be used to make up for the lack of enzyme activity in malt, improve wort components, increase beer fermentation degree, shorten fermentation cycle, and increase production. Enzyme preparations are generally used in liquid form, but once added in this form, it is difficult to separate and reuse. Because the price of enzyme preparations is generally relatively high, single use is not good for the cost, and these limiting factors have caused its application to be affected to a certain extent. [0003] Enzyme immobilization is one of the effective means to improve the above-mentioned enzyme preparation pro...

AI Technical Summary

Problems solved by technology

These conditions limit the polymer forming process and the enzymes cannot be directly immobilized in it.

In short, although people can prepare excellent carriers with different functions at present, the use of organic solvents or high temperature conditions are often involved in the preparation process of these carriers, which makes them difficult to be used as immobilized carriers for enzymes.