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Immune cell with anti-tumor function and application thereof

An immune cell, anti-tumor technology, applied to immune cells with anti-tumor function and its application fields, can solve the problems of low efficiency of malignant tumors, unsafe chimeric antigen receptor T cell immunotherapy, cytokine storm, etc. Low toxicity and side effects, inhibiting tumor growth and avoiding damage

Active Publication Date: 2017-05-31
NANJING UNIV
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Problems solved by technology

[0006] The purpose of the present invention is to overcome the inefficiency of traditional methods for treating malignant tumors and the unsafe and easily induced cytokine storm of the recently emerging Chimeric Antigen Receptor T cell immunotherapy (CAR-T), and provide a method for treating malignant tumors. Functional Immune Cells for Solid Tumors and Their Applications

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  • Immune cell with anti-tumor function and application thereof
  • Immune cell with anti-tumor function and application thereof
  • Immune cell with anti-tumor function and application thereof

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Embodiment Construction

[0043] Specific embodiments of the present invention will be described in detail below. It should be understood that the specific embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0044] In a preferred embodiment of the present invention, the present invention provides a recombinant expression vector containing mouse MCAD gene. Preferably, the recombinant expression vector is a lentiviral expression vector. There is no particular limitation on the lentiviral expression vector, as long as it can co-transfect packaging cells such as 293T packaging cells with the helper vector to obtain virus concentrates and cells that overexpress related proteins. Preferably, the lentiviral expression vector is pLenti6 / V5-D-TOPO-MCAD.

[0045] There is no particular limitation on the preparation method of the lentiviral expression vector pLenti6 / V5-D-TOPO-MCAD, which can be various methods conceiva...

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Abstract

The invention belongs to the field of medical biotechnology, and particularly relates to a genetically modified immune cell with a malignant solid tumor treating function and application thereof. The immune cell is a specific immune cell which is subjected to gene modification so as to over-express lipid metabolism-related genes. The tumor growth inhibiting capacity of the immune cell is realized by reducing accumulation of lipid droplets of the immune cell itself in a tumor microenvironment, reducing expression of tumor promotion genes / protein, and improving the phagocytotic ability, antigen presentation ability and tumor killing ability of the immune cell. The immune cell is an in-vitro NKT cell, a DC cell, a macrophage, a monocyte, a granulocyte or a T cell. Overexpression of related metabolism regulation genes enables the anti-tumor capacity of the immune cell to be improved remarkably. Compared with a chimeric antigen receptor T cell immunotherapy (CAR-T) which receives much attention currently, toxic and side effects are small, cytokine storm can not be caused, and clinical requirements can be met.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and specifically relates to a genetically modified immune cell with anti-tumor function and application thereof. Background technique [0002] Tumor has always been a major disease that plagues the world and seriously endangers human health. Therefore, it is an important research topic in the world medical community to find an effective tumor treatment method and completely conquer the tumor. At present, the traditional three main treatment methods are surgery, chemotherapy, and radiotherapy. Although these three main treatment methods are the basic means of global tumor treatment, their therapeutic effects are very limited. [0003] Recently, the adoptive immunotherapy of tumor cells is rising all over the world, bringing hope to cancer patients. At present, the cells used in adoptive immunotherapy of tumors are mainly monocytes, T cells or dendritic cells, such as T cells or mono...

Claims

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Application Information

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IPC IPC(8): C12N5/10A61K35/17A61K35/15A61P35/00
CPCA61K35/15A61K35/17C12N5/0636C12N5/0639C12N5/0642C12N5/0645C12N5/0646C12N2510/00
Inventor 沈萍萍牛志远
Owner NANJING UNIV
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