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Quality control method for artemisia rupestris herb extract

A quality control method and extraction technology, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as unreported

Active Publication Date: 2017-05-31
XINJIANG YINDUOLAN UIGHUR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Menghuaside is the main flavonoid component in Artemisia annua, but there is no report on the content determination method of Artemisia annua with Menghuaside as the reference substance.

Method used

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  • Quality control method for artemisia rupestris herb extract
  • Quality control method for artemisia rupestris herb extract
  • Quality control method for artemisia rupestris herb extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Determination of total flavonoids in Artemisia annua extract by ultraviolet spectrophotometry:

[0112] Preparation of the reference substance solution: take mongoside and weigh it accurately, add methanol to make a solution containing 25 μg per 1 mL of mongoside;

[0113] Preparation of the standard curve: Measure 2mL, 4mL, 6mL, 8mL, 10mL of the reference substance solution and place them in 10mL measuring bottles respectively, dilute to 10mL with methanol, use the corresponding reagent as a blank, and measure the absorbance at a wavelength of 320nm by UV spectrophotometry. Draw a standard curve with the absorbance as the ordinate and the concentration as the abscissa;

[0114] c. Preparation and determination of the test solution:

[0115] Take 6.0mg of this product, weigh it accurately, put it in a 10mL measuring bottle, extract it ultrasonically for 20 minutes with methanol, set the volume to 10mL at room temperature, take 0.5mL from it, put it in a 10mL measuring ...

Embodiment 2

[0117] Determination of total flavonoids in Artemisia annua extract by ultraviolet spectrophotometry:

[0118] Preparation of the reference substance solution: take mongoside and weigh it accurately, add methanol to make a solution containing 28 μg per 1 mL of mongoside;

[0119] Preparation of the standard curve: Measure 2mL, 4mL, 6mL, 8mL, 10mL of the reference solution and place them in 10mL measuring bottles respectively, dilute to 10mL with methanol, use methanol reagent as a blank, and measure the absorbance at 328nm wavelength by UV spectrophotometry. Draw a standard curve with the absorbance as the ordinate and the concentration as the abscissa;

[0120] Preparation and determination of the test solution: take 8.0 mg of Artemisia annua extract, accurately weigh it, put it in a 10 mL measuring bottle, use methanol for ultrasonic extraction for 20 minutes, set the volume to 10 mL at room temperature, and then accurately take 0.5 mL from it and place it in a 10 mL volume ...

Embodiment 3

[0122] Determination of the content of Menghuaside in Artemisia annua extract by high performance liquid chromatography:

[0123] Chromatographic conditions and system suitability:

[0124] Octadecylsilane-bonded silica gel is used as filler; acetonitrile is used as mobile phase A, 0.2% formic acid solution is used as mobile phase B, and gradient elution is carried out according to the table below; flow rate: 1mL / min; detection wavelength: 324nm; column temperature : 30°C, injection volume: 10μL, the number of theoretical plates should not be less than 4000 based on the calculation of the peak of mongoside;

[0125]

[0126] Preparation of the reference substance solution: take the reference substance of mongoside, accurately weighed, add methanol to make a solution containing 25 μg of mongoside per 1 mL;

[0127] Preparation of the test solution: take 30 mg of Artemisia argyi extract, accurately weigh it, put it in a 25 mL measuring bottle, add 20 mL of methanol for ultraso...

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Abstract

The invention discloses a quality control method for an artemisia rupestris herb extract. The method is characterized in that the content of general flavone in the artemisia rupestris herb extract is determined by taking linarin as a reference substance, and by using ultraviolet spectrophotometry. High performance liquid chromatography is utilized to assaying the content of the linarin. The quality control method provided by the invention is good in accuracy, stability and repeatability, the quality of the artemisia rupestris herb extract can be controlled effectively, and the quality control method can be applied to massive industrial production.

Description

technical field [0001] The invention relates to a quality control method of an Artemisia annua extract, belonging to the technical field of ethnic medicines. Background technique [0002] Artemisia rupestris L., a plant of the Compositae family, is used as medicine. It is called "Yizihu Emani" in Uighur and "Yizihu" in Kazakh. It has a long history of folk medicine in Xinjiang. It has the effects of clearing away heat and detoxification, invigorating the stomach, benefiting the gallbladder, and detoxifying snake poison. For indigestion, abdominal pain, hepatitis, urticaria, snake bites, cold and fever, etc. Mainly distributed in my country's Xinjiang, Central Asia, Europe and other places. This medicinal material is recorded in Zhao Xuemin's "Compendium of Materia Medica Supplements" in the Qing Dynasty of our country. It says: "Ba Likun produced a kind of medicine named Yizhi Artemisia. When horses go into the mountains, they collect grass and bring them home, and there...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 阿吉艾克拜尔·艾萨阿吉姑·阿布都热西提贺飞吴涛赵江瑜马庆苓
Owner XINJIANG YINDUOLAN UIGHUR MEDICINE
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