Fluorescent indicator detection method for concentration of free calcium ion in guard cell of tomato blade
A fluorescent indicator and guard cell technology, which is applied in the field of plant detection, can solve the problems of no optimization and large differences in detection sensitivity, and achieve the effect of convenient operation and accurate detection
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[0015] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0016] An embodiment of the present invention provides a fluorescent indicator detection method for free calcium ion concentration in tomato leaf guard cells, comprising:
[0017] Step 1, preparing a fluorescent indicator working solution with a set concentration; the preferred concentration of the fluorescent indicator working solution is 10 μM;
[0018] Step 2, obtaining the lower epidermis of tomato leaves;
[0019] Step 3, loading the prepared fluorescent indicator solution onto the obtained lower epidermis of tomato leaves;
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