Fluorescent indicator detection method for concentration of free calcium ion in guard cell of tomato blade

A fluorescent indicator and guard cell technology, which is applied in the field of plant detection, can solve the problems of no optimization and large differences in detection sensitivity, and achieve the effect of convenient operation and accurate detection

Inactive Publication Date: 2017-05-31
BEIJING UNIV OF AGRI
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Problems solved by technology

However, the current fluorescent indicator detection method has the problems of large differences in detection sensitivity in different plant tissues, differences in the lo

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  • Fluorescent indicator detection method for concentration of free calcium ion in guard cell of tomato blade
  • Fluorescent indicator detection method for concentration of free calcium ion in guard cell of tomato blade

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[0015] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0016] An embodiment of the present invention provides a fluorescent indicator detection method for free calcium ion concentration in tomato leaf guard cells, comprising:

[0017] Step 1, preparing a fluorescent indicator working solution with a set concentration; the preferred concentration of the fluorescent indicator working solution is 10 μM;

[0018] Step 2, obtaining the lower epidermis of tomato leaves;

[0019] Step 3, loading the prepared fluorescent indicator solution onto the obtained lower epidermis of tomato leaves;

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Abstract

The invention discloses a fluorescent indicator detection method for concentration of a free calcium ion in a guard cell of a tomato blade. The method comprises the steps of 1, preparing a fluorescent indicator working solution, 2, obtaining a tomato blade hypoderm, 3, loading the prepared fluorescent indicator solution on the obtained tomato blade hypoderm, and 4, acquiring a fluorescent image on the tomato blade hypoderm loaded with the fluorescent indicator solution by a laser-scanning confocal microscopy and determining an optimum method of fluorescent indicator detection for the concentration of the free calcium ion Ca<2+> in the guard cell of the tomato blade according to the acquired fluorescent image.

Description

technical field [0001] The invention relates to the field of plant detection, in particular to a fluorescent indicator detection method for free calcium ion concentration of tomato leaf guard cells. Background technique [0002] Ca 2+ As a second messenger in cells, it participates in the stomatal movement induced by hormones such as ABA. At the same time, many biotic or abiotic stresses can cause Ca in the cytoplasm of plant cells 2+ Concentration ([Ca 2+ ] cyt ) changes, plants will be based on [Ca 2+ ] cyt The difference in change decodes the different stimuli so that the cell responds accordingly. For example, when the guard cells on the leaves are stimulated by the outside world, their membrane potential will change, thereby causing [Ca 2+ ] cyt However, because each mature guard cell does not develop synchronously, each guard cell has its own unique signal transduction mode. A portion of guard cells [Ca 2+ ] cyt Under the induction of certain environment, it...

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
CPCG01N21/643G01N1/28G01N21/6458
Inventor 赵文超姚学辉杨瑞王建立赵福宽王绍辉
Owner BEIJING UNIV OF AGRI
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