Three-dimensional hyperspectral microscopic imaging system and imaging method

Abstract

The invention discloses a three-dimensional hyperspectral microscopic imaging system and an imaging method. The three-dimensional hyperspectral microscopic imaging system comprises a microscope, an aperture amplifying module, an imaging lens array, a broadband filter array, a sensor array and a controller, wherein the microscope is used for carrying out first-stage amplification on a microscopic sample and exporting an image plane from an image plane outgoing opening of the microscope; the aperture amplifying module is used for amplifying an aperture plane to a preset size; the imaging lens array is used for capturing different sub-aperture planes so as to generate space information in different angles and enabling images in corresponding angles of the microscopic sample to be formed behind each imaging lens; the broadband filter array is used for carrying out broadband spectrum modulation on the space information; the sensor array is used for collecting images generated after spectral encoding is carried out on the microscopic sample under observation at a preset angle; the controller is used for controlling the sensor array to be synchronously triggered so as to carry out iterative restoration and reconstruction through a four-dimensional deconvolution algorithm. According to the three-dimensional hyperspectral microscopic imaging system disclosed by the invention, collection of 30-Hz three-dimensional hyperspectral videos under optical microscopy can be realized, the imaging applicability is increased, and the use experience of a user is greatly increased.

Description

technical field [0001] The invention relates to the intersecting technical field of optics and informatics, in particular to a three-dimensional hyperspectral microscopic imaging system and an imaging method. Background technique [0002] With the development of biology and material science, more and more optical microscopic imaging needs to quickly acquire multi-dimensional visual information, such as spatial dimension, spectral dimension and temporal dimension, instead of traditional two-dimensional imaging. High-speed hyperspectral volume imaging has important applications in the fields of dynamic process observation of multiple fluorescent labels, high-throughput chromatography, medical science, and material analysis in changing environments. [0003] Although great progress has been made in fast volume imaging and fast hyperspectral imaging, no one has ever been able to achieve both at the same time, that is, fast hyperspectral volume imaging under an optical microscope...

AI Technical Summary

Problems solved by technology

This will have limitations for observing the dynamic process of biological samples. In order to ensure five-dimensional data sampling including time resolution, how to have sufficient instantaneous data throughput is a great challenge.

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