Application of bacillus coagulans in preparation of preparations for preventing or treating Parkinson's disease
A technology for Bacillus coagulans and Parkinson's disease, which is applied in the field of Bacillus coagulans in the preparation of preparations for preventing or treating Parkinson's disease, and can solve the problems of limited use, different sources of medicinal materials, and difficult to control symptoms.
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preparation Embodiment 1
[0033] Preparation Example 1 The preparation of bacillus coagulans powder
[0034] 1 Preparation of bacteria powder and identification of strains
[0035] Take samples of human feces, soil, haystack substrate, etc., and then put the samples in a sterilized bottle, take 2 grams of the sample and add it to 18mL of sterilized diluent, mix well, and carry out 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7Three dilution gradients, spread on the solid medium for selective single colony isolation of Bacillus coagulans, place in an incubator, culture at 37°C for 48 hours, select a single colony with good growth and inoculate it into the liquid expansion medium of Bacillus coagulans , expanded at 37°C for 48 hours. After the obtained culture solution is centrifuged (12000rpm) to separate the thalline, the thalline is freeze-dried in vacuum to prepare dry bacterial powder, and the number of viable bacteria is 1×10 9 CFU / g or ...
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