Bioreactor low-shearing lifting type ventilation stirring device

Abstract

The invention provides a bioreactor low-shearing lifting type ventilation stirring device. A defoaming chamber, a negative pressure stirrer, a ventilation chamber and a lower stirrer are positioned on the same axial line and are in sequential arrangement from top to bottom; a defoaming section communicates with a ventilation dispersion section by passing a foam overflowing conduit through a stirring section; a circle of defoaming net cover is arranged on the outer side of the defoaming chamber; three stirring pipes are uniformly arranged at the outer side of the negative pressure stirrer; an opening of the end part of the outer side of each stirring pipe is of an inclined surface structure; the other end of each stirring pipe communicates with a hollow shaft communicating to the tank bottom through the negative pressure stirrer; an air uniform distributer is arranged in the ventilation chamber. The culture liquid in a reaction tank is stirred by using a non-shearing structure; the stirring pipes are driven by rotating the hollow shaft, so that the pipe openings of the stirring pipes form differential pressure with the bottom end of the hollow shaft, so that the culture liquid realizes the circulation stirring in the tank body, so that the injury on the cell due to the shearing stirring is reduced; meanwhile, a small-size stirring blade is arranged at the bottom end of the stirring device and aims at realizing the better utilization of oxygen gas by cells for respiratory action; meanwhile, the deposition on the tank bottom of cells in the culture liquid is avoided.

Description

technical field [0001] The invention relates to a tank stirring device, in particular to a bioreactor low-shear lifting aeration stirring device, which belongs to the technical field of fermentation equipment. Background technique [0002] In the field of bioengineering technology, fermentation is the basis of biotechnology industrialization, the macrodynamics of biological reactions is the basis of fermentation process optimization, and the bioreactor is the external environment of the fermentation process, which directly affects the efficiency and optimization of the fermentation process. Ventilation and stirring fermentation equipment is widely used in fermentation equipment, but no matter what the ventilation method is, the air enters the equipment through a separate pipe from the outside to ventilate through the distributor. At present, for some special types of fermentation, due to the mechanical sensitivity of the cells, it is not suitable to use the paddle stirring, ...

AI Technical Summary

Problems solved by technology

At present, for some special types of fermentation, due to the mechanical sensitivity of the cells, it is not suitable to use the paddle stirring, because the paddle stirring is easy to cause damage to the cells, which is not conducive to the smooth progress of the fermentation. At the same time, the foam generated during the stirring process requires In addition, adding equipment for defoaming will increase equipment investment and make equipment operation complicated

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