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Method for detecting citrus iron chelate reductase family gene

A technology of reductase and iron chelation, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as death, quality deterioration, and photosynthetic rate reduction

Active Publication Date: 2020-09-15
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Since iron is an essential element for the synthesis of chlorophyll, iron deficiency in plants will result in abnormal chloroplast morphology and structure, reduced chlorophyll content, reduced photosynthetic rate, chlorotic yellowing of plant leaves, growth retardation or even death, resulting in reduced yield and poor quality of agricultural products. severe losses in agricultural production

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  • Method for detecting citrus iron chelate reductase family gene
  • Method for detecting citrus iron chelate reductase family gene
  • Method for detecting citrus iron chelate reductase family gene

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0027] The present embodiment detects the method for citrus iron chelating reductase family gene, comprises the following steps:

[0028] 1) Select 1-year-old seedlings of Ziyang orange, Carrizo citrange and Citrus trifoliate as materials for tissue expression analysis;

[0029] 2) Use the RN09-EASY spin plant RNA rapid extraction kit produced by Beijing Aidelai Technology Co., Ltd. to extract the RNA of each seedling root, stem, and leaf tissue; of course, other types of plants can also be used in different embodiments RNA extraction kit;

[0030] 3) Using the RNA extracted in step 2) as a template, the first strand of cDNA was synthesized by reverse transcription; the reverse transcription kit used in the synthesis of the first strand of cDNA in this example was produced by Baobio (Dalian) Co., Ltd. RT ReagentKit (Bao Biological Engineering (Dalian...

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Abstract

The invention discloses a method for detecting ferric-chelate reductase family genes of Citrus reticulata Blanco. The method comprises 1) selecting one-year-old seedlings of Ziyang orange, Carrizo citrange and trifoliate orange as tissue expression analysis materials, 2) extracting RNAs of the root, stem and leaf tissue of the seedlings, 3) carrying out inverse transcription synthesis through the RNAs as templates to obtain a first chain of cDNA, 4) carrying out fluorescence quantitative PCR, and 5) carrying out analysis on the test data through a 2 delta Ct method. The method realizes identification of expression conditions of five types of ferric-chelate reductase genes in different parts of Citrus reticulata Blanco through the specific primers and reaction system and is beneficial to research Citrus reticulata Blanco resistance to iron deficiency.

Description

technical field [0001] The invention relates to the technical field of iron chelating reductase gene detection, in particular to a method for detecting citrus iron chelating reductase family genes. Background technique [0002] In plants, iron, as the main component of porphyrin and the cofactor component of many important coenzymes, such as hemoglobin, peroxidase, flavoprotein, ferredoxin, etc., directly participates in photosynthesis, respiration, nitrogen assimilation , hormone synthesis, generation and removal of active oxygen, and defense against pathogen invasion. Since iron is an essential element for the synthesis of chlorophyll, iron deficiency in plants will result in abnormal chloroplast morphology and structure, reduced chlorophyll content, reduced photosynthetic rate, chlorotic yellowing of plant leaves, growth retardation or even death, resulting in reduced yield and poor quality of agricultural products. Agricultural production has caused serious losses. [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851C12Q1/6895
CPCC12Q1/6851C12Q1/6895C12Q2600/13C12Q2600/158C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 姚利晓陈善春何永睿许兰珍雷天刚邹修平彭爱红
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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