Method of improving phosphorus-enriching ability of aquatic plant

An aquatic plant and phosphorus-rich technology, applied in botany equipment and methods, biochemical equipment and methods, plant peptides, etc., can solve the problems of application limitations and achieve the effect of increasing the rate of phosphorus uptake

Inactive Publication Date: 2017-06-20
ZHEJIANG JUNE5 ENVIRONMENT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Method of improving phosphorus-enriching ability of aquatic plant
  • Method of improving phosphorus-enriching ability of aquatic plant
  • Method of improving phosphorus-enriching ability of aquatic plant

Examples

Experimental program
Comparison scheme
Effect test

application example 1

[0054] Do lateral flow test in a river, such as Figure 5 As shown, the water pump draws water from the river and sends it to two hydroponic ponds respectively for comparative experimental research.

[0055] Test time: September 2015; duration: 30 days; average temperature: 35 degrees.

[0056] Test water volume: 1m 3 / d; effective volume of hydroponic pool: 2m 3 ; Dimensions of hydroponic pool: 1×2×1.2Hm; Effective water depth: 1m.

[0057] Hydroponic pool A: plant common foxtail algae, 50 plants with a plant length of 20cm.

[0058] Hydroponic pond B: plant modified foxtail algae, 50 plants, plant length 20cm.

[0059] See the results of the operation Figure 7 .

[0060] Depend on Figure 7 It can be seen that the effect of genetically modified foxtail algae is good.

application example 2

[0062] Comparison of the effects of engineering tail water treatment in an aquaculture project, such as Image 6 As shown, the fish pond tail water is pretreated in the sedimentation tank first, and then enters wetland A and wetland B in parallel for treatment before being discharged.

[0063] Operating water volume: average daily water volume 20m 3 / d, the maximum flow rate is 2m 3 / h;

[0064] Sedimentation tank: size 4×2×3Hm, quantity 2;

[0065] Wetland (A or B): size 4×25×1.5Hm, number 2; effective water depth 1.0m; filler material is 10-30mm gravel; wetland A is planted with common foxtail; wetland B is planted with transformed foxtail.

[0066] Test time: September-October 2016; average temperature: 30-35 degrees.

[0067] See the results of the operation Figure 8 .

[0068] Depend on Figure 8 It can be seen that the effect of the genetically modified foxtail algae is good, and the phosphorus absorption is enhanced, which is helpful for the removal of COD.

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Abstract

The invention relates to a method of improving phosphorus-enriching ability of an aquatic plant. The method improves the phosphorus-enriching ability by increasing overexpression of a PHR1 transcription factor and wiping out a PHO2 gene. The method specifically comprises the following two steps: (1) increasing overexpression of the PHR1 transcription factor; and (2) wiping out the PHO2 gene. By genetically modifying the aquatic plant and copying and cultivating the aquatic plant by means of the method, the aquatic plant which reaches a certain quantity is placed in a to-be-repaired phosphorus-enriching water body to excessive absorb phosphorus in the water body; and in the absorption process, vegetables are bred massively, so that the phosphorus-absorbing rate is increased, and therefore, phosphorus meeting the standard is realized.

Description

technical field [0001] The invention relates to a plant transformation method, in particular to a method for improving the phosphorus-enriching ability of aquatic plants. Background technique [0002] Eutrophication is a water pollution phenomenon caused by excessive nitrogen, phosphorus and other elements. At present, in terms of water body treatment and water pollution control, more attention is paid to nitrogen control, and phosphorus control is relatively weak. But the facts show that the eutrophication threshold of phosphorus is less than one-tenth of that of nitrogen. Therefore, in the process of water eutrophication control and restoration, controlling phosphorus can achieve twice the result with half the effort. [0003] The Chinese invention patent with the patent number 01010251475.X discloses a phosphorous deficiency response regulatory protein, its coding gene and its application. In short, the application of the invention is limited to plant breeding; the inv...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/00
CPCC12N15/8243C07K14/415
Inventor 徐颋钱伟强寿鹿李育才周琪张海洋
Owner ZHEJIANG JUNE5 ENVIRONMENT TECH CO LTD
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