Method for detecting group B streptococcus infections

A technology of type B streptococcus and a detection method, which is applied in the field of detection of type B streptococcus infection, can solve the problems of high cost, time-consuming and high accuracy of the measurement method

Inactive Publication Date: 2017-07-07
TIANJIN BAODI HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many detection methods for type B streptococcal infection in clinical laboratories, including bacterial culture method, vaginal swab immunochromatography method, gold standard method, vaginal swab PCR method and other detection methods. Some methods have high accuracy bu

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  • Method for detecting group B streptococcus infections
  • Method for detecting group B streptococcus infections
  • Method for detecting group B streptococcus infections

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Embodiment 1

[0021] 1. In the detection method of the present invention, take the biological slice and place it at room temperature, add 25 μL serum sample to 25 μL PBS, add 30 μL diluted serum to 300 μL adsorbent, mix thoroughly, and centrifuge the treated serum at 13000 rpm for 10 minutes to remove the precipitate. Negative and positive controls do not need adsorbent treatment; add 25 μL of adsorbent-treated serum to each well of the slide, room temperature for 60 minutes, wash with 0.01mol / L PBS for 2 minutes for 3 times, add fluorescein isothiocyanate-labeled sheep Anti-human IgM globulin, wash at 20-37°C for 1-2 hours, wash with 0.01mol / L PBS for 2 minutes and 4 times; mount the slides, observe under a fluorescence microscope at 10×40 times; fluorescein isothiocyanate is excited at 490-495 nm Fluorescence is emitted at 520-530nm. If it is positive, it will be yellow-green, and if it is negative, there will be no yellow-green fluorescence. This method is compared with the PCR method for...

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Abstract

The invention discloses a method for detecting group B streptococcus infections and belongs to methods for determining characteristics of in-vivo blood. According to the method, a kit contains reagents for detection, such as a negative control serum, a positive control serum, a concentrated phosphate buffer, an adsorbent, isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin and a mounting medium and a biological thin sheet, and reaction areas of the biological thin sheet are coated with group B streptococci. A determination method comprises the steps of adding an IgG antibody removed diluted serum into the reaction areas, carrying out incubation, rinsing and spin-drying, then, adding the isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin, forming isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin fluorescent complexes around thalli, and generating green-yellow fluorescence. According to the method disclosed by the invention, indexes such as sensitivity and specificity are all higher than that of the existing methods.

Description

technical field [0001] The invention relates to a detection method for measuring blood characteristics in vivo, in particular to a detection method for type B streptococcus infection. Background technique [0002] Type B (beta) streptococcus (GBS) is a common intestinal bacteria, about 10-35% of healthy women can find traces of this bacteria in the intestinal tract or vagina. Opportunities expose fetuses and newborns to the source of infection, so that nearly 1% of newborns are infected, which poses a great threat to the life of the newborn. It is often the culprit of neonatal infection. If the genitourinary system of a pregnant woman contains type B streptococcus, although it will not cause disease itself, it may be passed on to the child vertically, which may be fatal in severe cases, causing neonatal infection, sepsis and neonatal infection. Meningitis is generally related to premature birth, low birth weight, and early water breakage. It occurs within one week after bir...

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56944G01N2333/315
Inventor 丁弘唐克健李铮李立和
Owner TIANJIN BAODI HOSPITAL
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