Method for detecting group B streptococcus infections

Abstract

The invention discloses a method for detecting group B streptococcus infections and belongs to methods for determining characteristics of in-vivo blood. According to the method, a kit contains reagents for detection, such as a negative control serum, a positive control serum, a concentrated phosphate buffer, an adsorbent, isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin and a mounting medium and a biological thin sheet, and reaction areas of the biological thin sheet are coated with group B streptococci. A determination method comprises the steps of adding an IgG antibody removed diluted serum into the reaction areas, carrying out incubation, rinsing and spin-drying, then, adding the isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin, forming isosulfocyanic acid fluorescein-labeled sheep anti-human-IgM globulin fluorescent complexes around thalli, and generating green-yellow fluorescence. According to the method disclosed by the invention, indexes such as sensitivity and specificity are all higher than that of the existing methods.

Description

technical field [0001] The invention relates to a detection method for measuring blood characteristics in vivo, in particular to a detection method for type B streptococcus infection. Background technique [0002] Type B (beta) streptococcus (GBS) is a common intestinal bacteria, about 10-35% of healthy women can find traces of this bacteria in the intestinal tract or vagina. Opportunities expose fetuses and newborns to the source of infection, so that nearly 1% of newborns are infected, which poses a great threat to the life of the newborn. It is often the culprit of neonatal infection. If the genitourinary system of a pregnant woman contains type B streptococcus, although it will not cause disease itself, it may be passed on to the child vertically, which may be fatal in severe cases, causing neonatal infection, sepsis and neonatal infection. Meningitis is generally related to premature birth, low birth weight, and early water breakage. It occurs within one week after bir...

AI Technical Summary

Problems solved by technology

[0003] There are many detection methods for type B streptococcal infection in clinical laboratories, including bacterial culture method, vaginal swab immunochromatography method, gold standard method, vaginal swab PCR method and other detection methods. Some methods have high accuracy but are time-consuming. The cost is high, some methods are simple, fast, and the results are more accurate. The present invention studies a method for measuring anti-type B streptococcus anti-IgM antibodies in blood to determine whether pregnant women are infected with type B streptococci.

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