A kind of bacillus amyloliquefaciens bacterial strain and its application for biocontrol

Abstract

The invention discloses a Bacillus amyloliquefaciens strain for biocontrol and its use. The strain Bacillus amyloliquefaciens B4 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No: 13466. The present invention includes isolation and cultivation of bacterial strains and 16S rDNA molecular identification; after Bacillus amyloliquefaciens is cultivated, different active substances (including its fermentation liquid, supernatant, and bacterial suspension) are obtained; in vitro, different active products have different effects on common fruit Inhibitory effect of spoilage fungi (Penicillium, Alternaria) on the surface; in vivo, antiseptic application in storage of loquat, pear, cherry tomato, and apple. The amyloliquefaciens strains and products thereof screened by the present invention can effectively inhibit the postharvest spoilage of various fruits, and can be used to prepare a fresh-keeping product for biocontrol that inhibits spoilage bacteria such as Penicillium and Alternaria and has a wide antibacterial spectrum agent.

Description

technical field The invention relates to the field of biotechnology in which a strain of Bacillus amyloliquefaciens can prevent and treat various postharvest diseases of fruits, and specifically applies a strain of Bacillus amyloliquefaciens for biocontrol and its application in fruit storage. Background technique The huge loss caused by postharvest diseases of fruits has become a concern at home and abroad. In developed countries, about 10% to 30% of fresh fruits are lost due to rot due to postharvest diseases, and postharvest rot is more serious in developing countries. At present, the prevention and control methods of postharvest diseases of fruits mainly include chemical fungicides, controlled atmosphere storage, low temperature disease prevention, heat treatment, etc. Among them, chemical antisepsis has the best effect. Biological control does not have the problems of environmental pollution, pesticide residues and continuous use of chemical resistance caused by chemica...

AI Technical Summary

Problems solved by technology

However, there are few reports on the application of Bacillus amyloliquefaciens cells to antiseptic preservation of various fruits after harvest. The present invention mainly uses different active components of Bacillus amyloliquefaciens as preservatives and sprays them on the surface of various perishable fruits. , such as loquat, pear, apple, cherry tomato, to observe its fresh-keeping effect

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