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A kind of method that applies supergravity technology to prepare nano liposome

A nanoliposome and supergravity technology, applied in the fields of biomedicine and cosmetics, can solve the problems of narrow particle size distribution of nanoliposomes, short production time, difficult industrial production of nanoliposomes, etc. Low cost and low energy consumption

Active Publication Date: 2021-01-12
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method utilizes the high-gravity rotating bed technology, which can greatly enhance mass transfer and micro-mixing, is beneficial to scale-up, has short production time, is easy to produce on a large scale, and the prepared nano-liposomes have a narrow particle size distribution, which is a good solution to the problem of nano-lipids. The problem that the body is difficult to industrialize

Method used

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  • A kind of method that applies supergravity technology to prepare nano liposome
  • A kind of method that applies supergravity technology to prepare nano liposome
  • A kind of method that applies supergravity technology to prepare nano liposome

Examples

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Comparison scheme
Effect test

Embodiment 1

[0037] Dissolve 80g of phosphatidylcholine and 10g of cholesterol in 2L of ethanol, add 5g of potassium dihydrogen phosphate and 5g of dipotassium hydrogen phosphate into 20L of water and adjust the pH to 6.5, turn on the high-gravity rotating bed and adjust the speed to 2000rpm, and use 2ml of ethanol / min, the water phase is fed at 20ml / min, and the temperature of the control system is 30°C. After the ethanol phase is fed, the high-gravity rotary bed is closed, and 300g sucrose is added to the resulting mixed solution, and the organic solvent is removed after freeze-drying. A dry freeze-dried powder is obtained. The freeze-dried powder can be stored for a long time, and the dried freeze-dried powder is reconstituted and hydrated with pure water before use to obtain a nanoliposome solution. figure 1 A scanning electron micrograph of the nanoliposome prepared in Example 1 of the present invention is shown. It can be seen from the figure that the average particle size of the n...

Embodiment 2

[0041] Dissolve 70g of phosphatidylcholine and 10g of cholesterol in 2L of isopropanol solution, dissolve 4g of potassium dihydrogen phosphate and 6g of disodium hydrogen phosphate in 20L of water, adjust the pH of the buffer to 7.4, and turn on the high-gravity rotating bed to adjust the speed to 2000rpm. The isopropanol phase is fed at 3ml / min, the water phase at 30ml / min, and the temperature of the control system is 30°C. After the isopropanol phase is fed, the supergravity rotary bed is closed, and 300g of sucrose is added to the resulting mixed solution. After freeze-drying, the solvent is removed to obtain dry nano-liposome freeze-dried powder. The freeze-dried powder can be stored for a long time, and the dried freeze-dried powder is reconstituted and hydrated with pure water before use to obtain a nanoliposome solution. The average particle diameter of the nanoliposome is 80nm, and its particle size distribution test shows that its PDI value is 0.16, showing good dispe...

Embodiment 3

[0043] Dissolve 70g of phosphatidylcholine and 15g of cholesterol in 2L of tert-butanol solution, dissolve 3g of disodium hydrogen phosphate and 2g of phosphoric acid in 10L of water and adjust the pH of the buffer to 5, turn on the supergravity rotating bed and adjust the speed to 1000rpm, t The butanol phase is fed at 3ml / min, the water phase is fed at 30ml / min, and the temperature of the system is controlled at 30°C. After the organic solution is fed, the high-gravity rotating bed is closed, and 300g trehalose is added to the resulting mixed solution. After drying, the solvent is removed to obtain dry nanoliposome freeze-dried powder. The freeze-dried powder can be stored for a long time, and the dried freeze-dried powder is reconstituted and hydrated with pure water before use to obtain a nanoliposome solution. The average particle diameter of the nanoliposome is 65nm, and its particle size distribution test shows that its PDI value is 0.2, showing good dispersion stabilit...

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Abstract

The present invention discloses a method for preparing nanometer liposome by using a supergravity technology. The method comprises: mixing an organic solution containing a fat material and an aqueous solution containing a carrier by using a supergravity rotation bed, and carrying out freeze drying treatment to obtain the nanometer liposome. According to the present invention, by using the excellent mass transfer ability of the supergravity rotation filling bed, the preparation process of the nanometer liposome is performed under the highly-microscopic and uniform condition so as to obtain the nanometer liposome particles with characteristics of controlled particle size, narrow distribution and high stability; by adding the freeze-drying protection agent, the breaking aggregation of the nanometer liposome during the freeze-drying process can be avoided, and the process for forming the nanometer liposome through the hydration of the freeze-dried product can be accelerated; the organic solvent is treated by using the freeze-drying method, such that the operation is simple, and the organic solvent removing effect is good; the method has advantages of simple process, less energy consumption, high efficiency, low cost, and easy large-scale industrial production; and the average particle size of the obtained nanometer liposomes is 20-200 nm, the PDI is 0.1-0.3, and the method is suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical fields of biomedicine and cosmetics. More specifically, it relates to a method for large-scale application of high-gravity technology to prepare nano-liposomes. Background technique [0002] Liposomes are closed vesicle-like structures formed by phospholipid bilayers. According to their structure, they can be divided into unilamellar vesicles (SUV), multilamellar vesicles (LUV) and multivesicular vesicles (MIV). Liposome was first discovered by the British AlecD.Bangham in 1965. Since then, it has been found that liposome has great application value as a material carrier, especially a drug carrier, so liposome has been systematically and extensively studied. [0003] After more than 20 years of exploration, researchers have proposed many valuable liposome preparation methods. At present, liposomes are mainly prepared by dispersion technique, which can be divided into three categories: 1) Based on mechanical dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K8/14A61K47/26A61K47/10A61K47/32A61K8/34A61K8/60A61K8/81
CPCA61K8/14A61K8/34A61K8/60A61K8/81A61K9/1277A61K47/10A61K47/26A61K47/32
Inventor 乐园刘亚萍王文龙吴凯樊蓉蓉张德涛王洁欣陈建峰
Owner BEIJING UNIV OF CHEM TECH
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