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SNP markers for identifying cynomolgus monkey origin (subpopulation), and applications thereof

A cynomolgus monkey, SNP14 technology, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve urgent problems

Inactive Publication Date: 2017-07-14
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

However, compared with the current advanced molecular detection technology and the leading level of international counterparts, my country's genetic monitoring standards still need to be improved, and it is urgent

Method used

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  • SNP markers for identifying cynomolgus monkey origin (subpopulation), and applications thereof

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Effect test

Embodiment 1

[0069] 1. Experimental animal samples

[0070] 20 cynomolgus monkeys of known origin (subgroup) (including the Philippines, Malaysia, Mauritius, Indochina, etc.); 400 cynomolgus monkeys of unknown origin (subgroup);

[0071] 2. Source of animal blood samples: Landao Biotechnology Co., Ltd.

[0072] 3. Screening process of target SNP

[0073] 3.1. Sequencing

[0074] First, a reduced genome library consisting of DNA samples from 20 cynomolgus monkeys from four different regions was sequenced.

[0075] The DNA sample concentration was set to 90ng / μL, digested with HinfI enzyme respectively, and then the digested products were cleaned, and the washed fragments were subjected to electrophoresis, and the bands between 200 and 500 bp were recovered, and the above steps were repeated until the raw materials were recovered sufficiently, and quantitative adjustment was performed again Synthesize the solution, and then take equal volumes from the 20 recovered and processed samples an...

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Abstract

The invention discloses SNP markers for identifying cynomolgus monkey origin (subpopulation), and applications thereof. The SNP marker comprises 48 SNP sites including SNP1 to SNP48 which respectively represents 61th site of nucleotide sequences shown by SEQ ID NO:1 to SEQ ID NO:48. 48 SNP markers for identifying the cynomolgus monkey origin (subpopulation) can be developed by utilizing the advantages of biological information technology, and the application of the SNP markers can greatly reduce the cost and simplify the operations for identifying the true origin of the imported cynomolgus monkey origin (subpopulation). The applications of the SNP markers can achieve the purposes of rapidly performing primary screening and lowering the detection cost, so that the standardized progress for identifying the cynomolgus monkey origin (subpopulation) can be promoted.

Description

technical field [0001] The present invention relates to utilizing the advantages of genome biological information analysis technology, in particular to a SNP marker for identifying the origin (subgroup) of cynomolgus monkeys and its application; 48 SNP markers of cynomolgus monkeys are screened out through high-throughput sequencing , used to distinguish cynomolgus monkeys of different origins (subgroups). Background technique [0002] At present, the domestic biomedical industry is developing rapidly. As an important supporting condition for the biomedical industry and an important material for life science research, experimental animals are directly related to the reliability and authenticity of experimental results. The genetic quality of experimental animals is one of the important factors for the quality of experimental animals. Only the use of experimental animals with stable genetic characteristics can ensure the comparability of biomedical research results and the re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 杜红丽曾玉琳蒙裕欢黄韧
Owner SOUTH CHINA UNIV OF TECH
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