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Application of TALE protein AvrXa7 in prevention and control of citrus bacterial canker disease

A technology of citrus canker, 1.TALE, applied in application, chemicals for biological control, animal repellent, etc.

Inactive Publication Date: 2017-07-25
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of TALE protein AvrXa7 of Xanthomonas oryzae for the control of citrus canker

Method used

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  • Application of TALE protein AvrXa7 in prevention and control of citrus bacterial canker disease
  • Application of TALE protein AvrXa7 in prevention and control of citrus bacterial canker disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] (1) Expression of AvrXa7 in wild-type X. citri

[0009] The avrXa7 gene was cloned into the plasmid pUFR034, and entered into the wild type Xac29-1 of X. citri by electrotransformation. After cultured in NB liquid medium, citrus leaves were inoculated by injection. After 5 days, the development of ulcer symptoms was observed. In the area inoculated with the wild-type strain, ulcer symptoms can generally be seen after 3 days, and the ulcer plaques in the injection inoculated area rise after 5 days; after inoculation with the ulcer strain expressing AvrXa7, the formation of ulcer symptoms cannot be observed ( figure 1 ).

[0010] (2) AvrXa7 against X. citri pthA4 Disturbance of gene pathogenic function

[0011] After citrus plants were inoculated with X. citrus strains carrying AvrXa7, transcriptomics methods were used to analyze the gene expression of host plants, and a group of avrXa7 and pthA4 related genes, the most prominent of which are related to pthA4 ...

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PUM

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Abstract

The invention provides application of TALE protein AvrXa7 in prevention and control of the citrus bacterial canker disease, and belongs to the technical field of biology. According to the application, TALE protein AvrXa7 separated from xanthomonas oryzae is used for inhibiting occurrence of the bacterial canker disease by interfering activation of citrus bacterial canker bacterium pthA4 on a susceptible gene and effectively inhibiting formation of canker symptom by expressing AvrXa7 in biological-control pseudomonas fluorescens Pf, is an optimal gene resource for constructing an efficient gene engineering strain, and has an important application value in biological prevention and control of citrus bacterial canker.

Description

technical field [0001] The patent belongs to the field of biotechnology and relates to the application of TALE protein AvrXa7 in preventing and treating citrus canker disease. AvrXa7, derived from Xanthomonas oryzae, can inhibit the pathogenic function of PthA4 in X. citri, interfere with the expression of susceptible genes in citrus, and thus effectively control the occurrence of citrus canker. Background technique [0002] Citrus canker is an important bacterial disease in citrus producing areas in my country and is subject to quarantine at home and abroad. The pathogen causing canker is Xanthomonas citri ( Xanthomonas citri subsp. citri ), four of which have been sequenced in Xanthomonas citri avr / pthA gene family members pthA1 , pthA2 , pthA3 and pthA4 ,in pthA4 It is the main pathogenic gene of the pathogenic bacteria, and the pathogenicity of the pathogenic bacteria on the citrus host is completely lost after the mutation of the gene. In the process of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P1/00
CPCA01N63/10
Inventor 邹华松孙东玲卓涛范晓静户勋
Owner FUJIAN AGRI & FORESTRY UNIV
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