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Cancer driving gene screening method based on gene mutation frequency

A technology of driving genes and screening methods, applied in the field of cancer medicine, can solve problems such as not considering the inherent properties of genes and affecting the frequency of gene mutations

Active Publication Date: 2017-07-25
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

This method only relies on the mutation rate of the gene, and does not consider some inherent properties of the gene and can affect the frequency of gene mutation

Method used

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  • Cancer driving gene screening method based on gene mutation frequency
  • Cancer driving gene screening method based on gene mutation frequency
  • Cancer driving gene screening method based on gene mutation frequency

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Embodiment Construction

[0092] The specific embodiment of the present invention is described in detail below in conjunction with summary of the invention:

[0093] The following is a detailed introduction to the screening process of lung cancer driver genes, which includes the data extraction process and data processing process, and finally screens out the driver genes. The present invention further illustrates the purposes and usage methods of the present invention through the following cases, but the present invention is not limited thereto.

[0094] 1. Experimental materials and environment configuration

[0095] Tumor and normal cell DNA from 177 lung cancer patients.

[0096] Software system: Linux system

[0097] Software required for data processing: BWA, Samtools, Picard, GATK, Annovar, Oncotator, downloaded from the official website of the tool.

[0098] R: open source computing platform, download from R official website

[0099] 2. Experimental method

[0100] (1) Acquisition of tumor ...

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Abstract

The invention discloses a cancer driving gene screening method based on gene mutation frequency and belongs to the field of cancer medicine. The method comprises the steps that (1) tumor gene mutation data is acquired; (2) the mutation data is preprocessed; (3) adjacent genes of each gene are screened; (4) background mutation data of each kind of mutation is calculated; and (5) hypothesis testing is used to screen mutation genes according to mutation values. According to the method, a modern high-throughput sequencing technology, DNA data processing software, a classic clustering algorithm and a classic statistical method are utilized and combined with biotic factors influencing gene mutation, so that screening of cancer driving genes is more precise, and the method has an important meaning for both research and development of novel anti-cancer drugs and clinical diagnosis and treatment of cancers.

Description

technical field [0001] The invention belongs to the field of cancer medicine and relates to a method for screening cancer driver genes based on gene mutation frequency. Background technique [0002] Cancer is caused by somatic mutations. Carcinogenic factors induce normal cells to mutate. When some specific gene sites in cells mutate, it will greatly promote the generation and development of cancer cells. These special genes are called driver genes. Driver gene mutations play a key role in the development of cancer and are the most important cause of cancer. [0003] Not all mutated genes detected in tumor cells are driver genes. Corresponding to the driver gene is the passenger gene, and passenger gene mutations have very little impact on the occurrence and development of cancer. Therefore, how to effectively identify cancer driver genes is still an urgent problem in current cancer research. The screening of cancer driver genes plays an important role in the development...

Claims

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Application Information

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IPC IPC(8): G06F19/00G06F19/20G06F19/24
CPCG16B25/00G16B40/00G16H50/30Y02A90/10
Inventor 顾宏秦尤秦攀曹隽喆
Owner DALIAN UNIV OF TECH
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