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Applications of DCF1 gene knockout in preparation of drugs for alleviating Alzheimer's disease

A kind of Alzheimer's and gene technology, applied in the application field of knocking out DCF1 gene in the preparation of drugs for alleviating Alzheimer's symptoms

Inactive Publication Date: 2017-07-28
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the study of the relationship between DCF1 and AD in a complete animal model has not yet been published
In view of the fact that the existing AD treatment drugs do not have the desired effect, and gene therapy may be the key treatment method to achieve a breakthrough, we crossed the existing mice in the laboratory with the introduced AD model mice to obtain a knockout AD model mice with dcf1 gene

Method used

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  • Applications of DCF1 gene knockout in preparation of drugs for alleviating Alzheimer's disease
  • Applications of DCF1 gene knockout in preparation of drugs for alleviating Alzheimer's disease

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Embodiment Construction

[0017] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples. For the experimental methods that do not specify specific conditions in the following examples, select according to conventional methods and conditions, or according to the product instructions.

[0018] 1. Preparation of frozen sections:

[0019] (A) Prepare 4% PFA for reagents and materials: 0.2M PB250ml, NaCl 4.5g, add 200ml ddH2O, put in a shaker at 37°C, wait until PFA is completely dissolved, dilute to 500ml, store in a refrigerator at 4°C, generally can be stored for 2 -3 months. 0.9% NaCl: Weigh 9g NaCl, add 900ml ddH2O, dilute to 1L, store at 4°C. 20% sucrose solution: Weigh 100g sucrose, add 0.2MPB25ml, NaCl4.5g, add 200mlddH2O, wait for the sucrose to dissolve, and dilute to 500ml. 30% sucrose solution: Weigh 150g sucrose, add 0.2MPB25ml, NaCl 4.5g, add 200mlddH2O, wait for the sucrose to dissolve, and dilute to 50...

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Abstract

The present invention relates to applications of DCF1 gene knockout in preparation of drugs for alleviating Alzheimer's disease. According to the present invention, mainly wild type mice, dcf1 knockout mice and AD model mice are adopted as control, and Dcf1 gene knockout AD model mice are adopted as a research object; the thioflavine staining results of the mouse brain freezing slices show that the deposition of senile plaques can be reduced after DCF1 gene knockout; the observation results of the mouse spatial learning and memory ability from the ethology prove that the learning and memory ability of the mouse can be enhanced after DCF1 gene knockout; and the results show that the DCF1 gene knockout can alleviate Alzheimer's disease, and the gene target is provided for the development of the new AD treatment drug.

Description

technical field [0001] The invention relates to the application of knocking out DCF1 gene in the preparation of medicine for alleviating Alzheimer's symptoms. [0002] Background of the invention [0003] AD (Alzheimer disease) is a common degenerative disease of the nervous system. Its clinical manifestation is mainly progressive dementia. , Reduced neurons. With the advent of an aging society, the incidence of AD continues to increase. According to statistics: there are more than 20 million AD patients in the world, and it is increasing at a rate of 4.6 million people every year. It has greatly reduced the quality of life and life expectancy of this population, and has caused serious social impact. Amyloid precursor protein (APP) can secrete β-amyloid 42 (amyloid-βprotein42, Aβ42) and β-amyloid 4 (0amyloid-βprotein 40, Aβ40) after being cleaved by proteolytic enzymes , and Aβ42 can lead to Alzheimer's disease (AD), and the occurrence of AD is mainly manifested by the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P25/28A61P25/00
CPCA61K45/00
Inventor 文铁桥李伟豪李聪王娇冯瑞丽
Owner SHANGHAI UNIV
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