Construction method of gene mutation detection library for tumor treatment cardiac toxicity prediction

A technology for cardiotoxicity and tumor treatment, applied in chemical libraries, microbial assay/test, biochemical equipment and methods, etc., can solve problems such as cancer treatment failure

Inactive Publication Date: 2017-07-28
CHONGQING CANCER INST
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  • Construction method of gene mutation detection library for tumor treatment cardiac toxicity prediction
  • Construction method of gene mutation detection library for tumor treatment cardiac toxicity prediction
  • Construction method of gene mutation detection library for tumor treatment cardiac toxicity prediction

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[0076] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0077] Construction method of gene mutation library for prediction of cardiotoxicity in tumor therapy, covering human genes KRAS, JAK3, AKT1, CREBBP, PTEN, RB1, TP53, CTNNB1, TSC2, TSC1, ERBB2, PIK3CA, SF3B1, JAK2, CDH1, SMAD4, GATA3, MAP2K4 A total of 56 genetic variation sites on , MAP3K1 and ESR1.

[0078] Specifically include the following steps:

[0079] (1) For target genes UGT1A6, FANCD2, ABCC5, PIK3R1, SLC22A7, VEGF, GSTA1, SLC22A16, ABCB1, CYP3A5, CYP3A4, SLC28A3, ESR2, AKR1C3, CYP2C8, ABCC2, CAT, GSTP1, SLCO1B3, RARG, SLC22A17, TCL1A, MRP1, ABCC1, NQO1, CYBA, HER2, RAPTOR, CYP2B6, CBR1, NCF4, RAC2, CYP2D6, CBR3, NOS3, ESR1, CELF4 and DPD design the basic amplification primer set, the forward primer and reverse primer set of the basic amplification primer set There are additional 2-5 Ts to the 5' end of the primer, and the ...

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Abstract

The invention discloses a construction method of a gene mutation detection library for tumor treatment cardiac toxicity prediction. The construction method is characterized by covering totally 56 genetic variation loci on 38 related genes of human tumor treatment cardiac toxicity. The construction method of the invention quickly completes the construction of the library by aiming at separately managing a plurality of target sequences, the construction process of the entire library only needs 2 to 3 hours, and only 45 min manually, so that the difficulty of detecting somatic cell polygenes and multiple target points required by predicting whether a greater cardiac toxicity is produced or not before a patient receives a tumor drug therapy clinically at present can be solved very effectively, and the cost is low.

Description

technical field [0001] The invention relates to a method for constructing a tumor treatment cardiotoxicity prediction gene mutation library for high-throughput sequencing detection. Background technique [0002] With the development of multidisciplinary comprehensive treatment and the promotion of early screening, cancer mortality has continued to decline in the past 30 years. However, the incidence of organ damage related to systemic therapy has continued to increase. With the prolonged survival of cancer patients, cardiovascular disease has become the second leading cause of death. Cardiovascular events may pose a significant risk to the survival and quality of life of cancer patients. The 5-year survival rate for early breast cancer has improved from 79% to 88%. It has been reported that cardiovascular mortality may become the leading cause of death in breast cancer patients 10 years later. Many studies have shown that about 75% of patients diagnosed with breast cancer...

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IPC IPC(8): C40B50/06C12Q1/68
CPCC40B50/06C12Q1/6886C12Q2600/142C12Q2600/156C12Q2600/16
Inventor 辇伟奇张娜李丽仙刘海霞葛闯曹勇唐万燕何永鹏
Owner CHONGQING CANCER INST
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