A method for efficient breeding of Danish hibiscus excellent strains
An excellent, strain-based technology, applied in horticultural methods, fertilization methods, botanical equipment and methods, etc., can solve problems such as restricting the development of the Danish hibiscus industry, and achieve the effects of being suitable for factory breeding, simple operation methods, and simple operation.
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Embodiment 1
[0036] The method for efficient breeding of a kind of Danish hibiscus excellent strain of the present embodiment comprises the following steps:
[0037] (1) Take materials
[0038] The leaves with petioles of the potted Danish hibiscus introduced from Denmark with excellent agronomic traits (one or more traits in high yield, large flowers, long flowering period, and good stress resistance) were selected as explants; wherein, The leaf with petiole is obtained as follows: the leaf with petiole is broken off from the joint between the Danish hibiscus stem and the petiole;
[0039] (2) Pretreatment of explants
[0040] The Danish hibiscus leaf with petiole in the step (1) is placed under running water and rinsed for 10 minutes, and then the surface is disinfected for 5 minutes by a potassium permanganate disinfection solution with a concentration of 0.5‰, and then dried in the shade after being pulled out, and set aside;
[0041] (3) Induction treatment of explant adventitious r...
Embodiment 2
[0048] The method for efficient breeding of a kind of Danish hibiscus excellent strain of the present embodiment comprises the following steps:
[0049] (1) Take materials
[0050]Select the stem section with leaves of the potted Danish hibiscus introduced from Denmark in the greenhouse with excellent agronomic traits (one or more of high yield, large flowers, long flowering period, and good stress resistance) as explants; , the stem section with leaves is drawn as follows: cut the stem section with scissors into micro-stem sections with 1 axillary bud and 1 leaf with a length of 1.0-3.0 cm;
[0051] (2) Pretreatment of explants
[0052] The stem section of Danish hibiscus leaves in step (1) is placed under running water and rinsed for 15 minutes, then the surface is disinfected for 10 minutes by a potassium permanganate disinfection solution with a concentration of 0.75 ‰, and then dried in the shade after being pulled out for subsequent use;
[0053] (3) Induction treatmen...
Embodiment 3
[0060] The method for efficient breeding of a kind of Danish hibiscus excellent strain of the present embodiment comprises the following steps:
[0061] (1) Take materials
[0062] Select the leaves with petioles and the stems with leaves of the potted Danish hibiscus introduced from Denmark in the greenhouse with excellent agronomic traits (one or more of high yield, large flowers, long flowering period, and good stress resistance) As an explant; Wherein, the method for drawing the leaves with petioles is: breaking off the leaves with petioles at the junction of the Danish hibiscus stem and the petiole; 1.0-3.0 cm long microstem segment with 1 axillary bud and 1 leaf;
[0063] (2) Pretreatment of explants
[0064] Put the leaves with petioles and the stems with leaves of the Danish hibiscus in step (1) under running water for rinsing for 20 minutes, then disinfect the surface with a potassium permanganate disinfection solution with a concentration of 1.0‰ for 15 minutes, ta...
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