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A kind of high-efficiency multiplication method of hybrid pennisetum

A high-efficiency technology for hybrid pennisetum, applied in the field of plant tissue culture, can solve the problems of impure varieties, miscellaneous plants, affecting the quality and yield of pennisetum planting, and achieve the effect of reducing production costs and stabilizing the propagation system

Active Publication Date: 2018-07-13
贵州博安生物科技有限责任公司
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  • Abstract
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Problems solved by technology

At present, due to the impurity of hybrid pennisetum, there is a certain proportion of hybrid plants, which affects the quality and yield of planted pennisetum

Method used

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  • A kind of high-efficiency multiplication method of hybrid pennisetum
  • A kind of high-efficiency multiplication method of hybrid pennisetum

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Embodiment Construction

[0020] The present invention will be further described below in conjunction with the examples, but the present invention is not limited to the following examples, it can be foreseen that those skilled in the art may produce various changes in the implementation under the condition of combining the prior art.

[0021] A method for efficiently multiplying hybrid pennisetum, the steps comprising:

[0022] 1) Selection and sterilization of explants

[0023] Take the young heart leaves of hybrid pennisetum, scrub the surface with 75% ethanol, soak in 75% ethanol for 30s, rinse with sterilized deionized water for 3 times, and rinse with 0.1% HgCl 2 Soak for 5-8 minutes, rinse with sterilized deionized water for 5 times, and dry the surface of the material with sterile filter paper.

[0024] 2) Callus induction

[0025] The sterilized heart leaves were cut into discs with a thickness of 0.2-0.5 cm and placed in the callus induction medium, cultured in the dark at 26-28 °C for 20-2...

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Abstract

The invention discloses a high-efficiency expanding propagation method for crossbred Chinese pennisetum, and belongs to the technical field of plant tissue culture. The high-efficiency expanding propagation method comprises the following steps: explant selection and sterilization, callus induction, embryonic callus induction and proliferation, multiple shoot induction, transplanting acclimatization, and the like. A stable and efficient expanding propagation system is established, the callus induction is up to 90%, the expanding propagation coefficient of the embryonic callus induction is as high as 20 times, the multiple shoot induction rate is up to 95%, and the acclimatization survival rate is up to 97%. Due to adoption of an ex vitro rooting mode, the production cost is greatly lowered, the method is applicable to industrialized seedling production, and assurance is provided or commercial planting of high-quality Chinese pennisetum.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for efficiently multiplying hybrid pennisetum. Background technique [0002] Pennisetum, perennial, with strong fibrous roots, erect culms, clustered, densely pilose under the inflorescence, leaf sheaths smooth, compressed on both sides, and the upper part of the culm is longer than the internode. It blooms in summer and sets seeds in autumn. It has the characteristics of high grass yield, strong stress resistance, good quality, and less pests and diseases. It is a high-quality green feed for herbivorous livestock and poultry and an ideal crop for water and soil conservation. [0003] In recent years, with the continuous improvement of hybrid pennisetum high-quality pulp, medium density fiberboard production technology and biomass energy conversion technology, the demand for hybrid pennisetum is increasing rapidly. At present, due to the impurity of the hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 刘红美梁大勇方小波石建龙
Owner 贵州博安生物科技有限责任公司
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