Fen Fangji's Seed Tissue Culture Propagation Method

Abstract

The invention discloses a seed tissue culture and propagation method of fourstamen stephania roots. The seed tissue culture and propagation method comprises six steps of seed storage, seed disinfection, initiation culture, induction culture, multiplication culture and rooting culture, and is characterized in that in the induction culture step, an induction culture medium I is used for culturing germinated and inflated seeds to induce aseptic seedlings and an induction culture medium II is used for continually culturing the aseptic seedlings so that the browning rate of the multiple shoots is reduced and the phenomenon that the multiple shoots are partially vitrified is eliminated; and in the multiplication culture step, two steps of multiplication culture are adopted: first step, a lot of multiple shoots can be rapidly cultured; and second step, strong multiple shoot seedlings for rooting and parts of newly-propagated multiple shoots can be cultured, so that the propagation efficiency of the tissue culture method is effectively improved. The method is simple in culture flow, high in propagation coefficient and convenient to operate, is suitable for industrialized seedling production and provides technical supports for industrially culturing fourstamen stephania root seedlings.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a seed tissue culture propagation method of Fenfangji. Background technique [0002] Fenfangji, also known as Hanfangji, or Baimuxiang, is the root of the Fangji plant Shitoad, which is a wild resource. It is a perennial vine that grows on the edge of grass and shrubs in hillsides and hills. The main producing areas are Jiangxi and Anhui. Inducing water to reduce swelling, dispelling wind and relieving pain. [0003] Fenfangji likes a moist and humid environment. It grows in the shade of shrubs, and is afraid of drought and waterlogging. Because the roots grow deep, it requires soft and sandy soil. The main sources of artificially cultivated seedlings are from two aspects. After germination into seedlings, it is transplanted and cultivated, but due to its low reproductive capacity, it is seldom used. The second is to use strong mature rhizomes to cultivate and germinate. ...

AI Technical Summary

Problems solved by technology

Through the investigation of the wild resources of Fangji in the above-mentioned areas, it is found that the natural reproduction and survival rate of Fangji is low, and the asexual reproduction of roots is the main method. It can only be excavated and used as medicine after more than 5 years, and the growth period is long.

At present, Fangji medicinal materials mainly come from the wild. Due to the limitation of provenance and long production cycle, artificial cultivation has not seen large-scale artificial cultivation. The price of this medicinal material has risen again and again in the past ten years. The predatory mining of resources has brought Fenfangji resources to the brink of depletion

Due to the low germination rate of Fenchia seed propagation, and the plants over 3 years old begin to bear fruit gradually, and rhizome propagation requires a large number of strong fresh rhizomes to cultivate and germinate, so the existing seed and rhizome propagation techniques are difficult to meet the provenance requirements of artificial cultivation For this reason, the use of tissue culture techniques of modern biotechnology to propagate Fenfang seedlings can solve the provenance problem of Fenfang artificially planted. It is one of the effective ways to solve the shortage of Fenfang resources by carrying out wild breeding or artificial semi-wild rearing.

Images