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Method for cultivating detoxification tissue culture bulb of hyacinth

A cultivation method and technology of daffodils, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of low reproduction rate, difficulty in separation and purification, and high virus rate, so as to achieve rapid proliferation, ensure safety and quality, and eliminate The effect of carry and spread

Inactive Publication Date: 2010-07-14
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a solution for the defects of low reproduction rate, high virus rate, compound infection of multiple filoviruses, and difficulty in separation and purification in the existing conventional production and propagation technology of daffodils. A method that can reproduce bulbs in large quantities and quickly, and can remove the virus carried by the bulbs and undergo specific detection to ensure the detoxification, tissue culture and detection of non-toxic narcissus bulbs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for cultivating the detoxified tissue-cultured bulbs of narcissus, carried out as follows:

[0042] (1) The preparation of the culture medium, including the basic medium and the components of the culture medium at each stage of tissue culture and the weight per liter are:

[0043] 1) Basic medium: MS medium for subbulb induction, proliferation and bulb growth medium, and 1 / 2 MS medium for rooting medium; wherein, the agar is 8g / L, and the pH is 5.7;

[0044]2) Daughter bulb induction medium: MS+BA 0.5mg / L and NAA 0.1mg / L of 30g / L sucrose;

[0045] 3) Daughter bulb proliferation medium: white sugar 40g / L MS+BA 0.1mg / L and NAA 0.5mg / L;

[0046] 4) Bulb growth medium: white sugar 40g / L MS+BA 0.05mg / L and NAA 0.1mg / L;

[0047] 5) Rooting medium: 1 / 2 MS+NAA 0.25mg / L of white sugar 20g / L.

[0048] (2) The cultivation of narcissus detoxified tissue culture bulbs:

[0049] 1) Bulb low-temperature treatment: the bulb is subjected to low-temperature treatment at 8°C ...

Embodiment 2

[0094] The agar in the basic medium of the present embodiment is 7g / L, and the pH is 5.6; the subbulb induction medium is: MS+BA 0.1mg / L and NAA 0.3mg / L of sucrose 20g / L; the subbulb proliferation medium is : white sugar 20g / L MS+BA 0.5mg / L and NAA 0.1mg / L; bulb growth medium: white sugar 30g / L MS+BA 0.02mg / L and NAA 0.25mg / L; rooting medium is: White sugar 40g / L 1 / 2 MS+NAA 0.45mg / L; bulbs were treated at 8°C for 2 weeks at low temperature; bulb shoots were soaked in 75% alcohol for 0.5 minutes, and 0.1% mercuric chloride aqueous solution for 10 minutes; induction, proliferation, The culture conditions of each stage of growth and rooting are: temperature 25±2° C., light intensity 1500 Lx, and light time 10 h / d;

Embodiment 3

[0096] The agar in the basic medium of this embodiment is 9g / L, and the pH is 5.8; the subbulb induction medium is: sucrose 40g / L MS+BA1mg / L and NAA 0.5mg / L; the subbulb proliferation medium is: white sugar 30g / L MS+BA 0.3mg / L and NAA 1mg / L; bulb growth medium: white sugar 20g / L MS+BA0.09mg / L and NAA 0.5mg / L; rooting medium: white sugar 30g / L 1 / 2 MS+NAA 0.1mg / L of L; bulbs were treated at 8°C for 4 weeks at low temperature; bulb shoots were soaked in 75% alcohol for 1.0 minutes, and 0.1% mercuric chloride aqueous solution for 15 minutes; induction, proliferation, growth, rooting The culture conditions of each stage are: temperature 25±2°C, light intensity 2500 Lx, light time 10h / d; other steps and processes are the same as in Example 1.

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Abstract

The invention relates to a culture method of hyacinth virus-free tissue culture bulb, which belongs to plant propagation technical field. The method comprises the steps of the preparation of a culture medium, the culture of the virus-free tissue culture bulb, the detection of a virus ELISA and so on. The invention can detect tissue culture bulb seedlings rapidly with high sensitivity and can stopthe virus from carrying and transmission. Comparing with the bulbs which are not virus-free, the virus-free tissue culture bulb has rapid growth speed, early flowering stage and bright flower colour.Virus-free tissue culture bulb breeds rapidly, the breeding ratio per month of which can reach 3-5 times more than the bulbs which are not virus-free. The invention is suitable for industrial seedling production and can be widely used in the purification, the rejuvenescence and the development of the hyacinth.

Description

technical field [0001] The invention relates to the technical field of plant virus-free tissue culture and rapid propagation, in particular to a method for cultivating and detecting the virus-free tissue culture bulbs of narcissus. Background technique [0002] Narcisses (Narcissus) is a general term for new varieties of narcissus introduced from Europe to my country. It is a perennial herb of the genus Narcissus in the family Amaryllidaceae. After the 1980s, large-scale introductions were carried out, mainly for spring flower exhibitions, and a small amount of potted plants were used as New Year's Eve flowers. Compared with Chinese narcissus, foreign narcissus has the characteristics of large flowers, bright colors, and pleasant fragrance; most varieties have trumpet-shaped corollas, and most of them are yellow, so they are also called "trumpet narcissus" or "yellow narcissus". Daffodils are also excellent garden ground cover flowers used as cut flowers. Daffodils are gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00C12N5/04A01H4/00
Inventor 陈剑平徐刚郑红英林林汪一婷程晔吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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