Staining method for digestive system of predatory mite

A technology of digestive system and dyeing method, which is applied in the direction of animal husbandry, etc., to achieve the effect of simple operation

Inactive Publication Date: 2017-08-08
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1
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  • Abstract
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Problems solved by technology

[0004] However, there is no report on how to easi

Method used

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Examples

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Embodiment

[0009] Example Dyeing method of predatory mite

[0010] At a temperature of 24°C and a humidity of 85%, pour 30ml of 0.1% sodium fluorescein into a petri dish with a diameter of 5cm, then stretch the parafilm film, cut out a circle with a diameter of 3cm, and then place the cut parafilm Place the membrane flat on the surface of the sodium fluorescein solution in the petri dish to make the parafilm float on the petri dish solution, and finally put the predatory mites that have been starved for 60 hours in a 2 ml centrifuge tube on the film, and the parafilm can be released in 1 hour. The digestive system of the predator mite was observed under a fluorescent stereomicroscope.

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Abstract

The invention relates to a staining method for a digestive system of a predatory mite. The method is characterized in that 0.05%-0.5% of a fluorescein sodium or fuchsine solution is poured into a petri dish at the temperature of 25-30 DEG C and the humidity of 75%-90%, then a well stretched parafilm is cut into a round piece with the diameter smaller than that of the petri dish, then cut parafilm is placed flatwise on the surface of the fluorescein sodium or fuchsine solution in the petri dish and is enabled to float on the solution in the petri dish, finally, the predatory mite starved for 0-72 h is placed on the parafilm, and the digestive system of the predatory mite can be examined under a microscope after 1-3 h. The method is simple to operate and can provide technical reference for observation of a digestive tract and the whole digestive system of the predatory mite.

Description

technical field [0001] The invention relates to a dyeing method for the digestive system of predatory mite, and belongs to the field of biological control of modern agricultural pests. Background technique [0002] At present, domestic research on predatory mites is mainly limited to classification, breeding and application. There is little research on the microstructure of predatory mites, especially the mechanism of the digestive system. [0003] The digestive system of the predatory mite is an important structure for studying the food that the predatory mite eats and digests. It is of great significance to observe the direction of the food in the mite body after the predatory mite eats the food. . [0004] However, there is no report on how to observe the digestive system of predatory mite conveniently. [0005] The purpose of the present invention is to provide a simple and efficient dyeing method for the digestive system of predatory mites without causing the death of...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王恩东徐学农吕佳乐周长青贾冰红刘珊尹哲庞博张乐张正伟宗静祝宝英
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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