Method for quantifying tissue culture seedling nitrate utilization efficiency

A tissue culture seedling and nitrate technology, applied in the field of plant biology, can solve the problems of complex experimental operation, large experimental error, difficulty in determining nitrate in large quantities of culture medium, etc., and achieve the effect of simple calculation method and few steps.

Active Publication Date: 2017-08-11
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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Problems solved by technology

The determination of nitrate in the traditional medium is not only complicated in the experimental operation, it is difficult to measure t

Method used

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  • Method for quantifying tissue culture seedling nitrate utilization efficiency
  • Method for quantifying tissue culture seedling nitrate utilization efficiency
  • Method for quantifying tissue culture seedling nitrate utilization efficiency

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Experimental program
Comparison scheme
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Embodiment Construction

[0028] Embodiments of the invention:

[0029] The first step is to obtain the clonal tissue culture seedlings of the investigated plants through plant tissue culture technology. Usually a well-grown bud can obtain a large number of high-quality clone tissue culture seedlings after about 4 generations of cultivation.

[0030] The second step is to configure culture media with different nitrate concentrations, and determine the stable nitrogen isotope value δ of the nitrate medicines used to configure the above culture media 15 N substrate ;

[0031] The third step is to inoculate the clonal tissue culture seedlings with 1 single bud in the above-mentioned medium with different nitrate concentrations, and cultivate them for 5 weeks;

[0032] The fourth step is to take out more than 100 mg of leaves of the above-mentioned clone tissue culture seedlings cultivated for 5 weeks, and measure the stable nitrogen isotope value δ of the tissue culture seedling leaves 15 N foliar ; ...

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Abstract

The invention discloses a method for quantifying the tissue culture seedling nitrate utilization efficiency. Clone tissue culture seedlings of inspected plants are obtained through tissue culture; the clone tissue culture seedlings are inoculated onto culture media with different nitrate concentrations respectively for culture; stable nitrogen isotope values of nitrate drugs and tissue culture seedling blades are determined; the tissue culture seedling isotope assimilation ratio under all nitric acid concentration gradients is worked out; a relational model of the tissue culture seedling isotope assimilation ratio and the nitrate concentrations is established according to a michaelis-menten equation of the enzyme kinetics; the model is subjected to derivation, and a tissue culture seedling nitrate utilization efficiency equation is obtained; according to the tissue culture seedling nitrate utilization efficiency equation, the tissue culture seedling nitrate utilization efficiency and the maximum utilization efficiency of nitrate are determined. According to the method, few materials and steps are needed, the calculation method is simple, and online dynamic monitoring can be conducted; the monitoring result can be the supply quantity for effectively managing the culture medium nitrate.

Description

technical field [0001] The invention relates to a method for quantifying nitrate utilization efficiency of tissue culture seedlings, belonging to the field of plant biotechnology. [0002] technical background [0003] Nitrate is an important nitrogen source in plant tissue culture. Nitrate plays an important role in the growth and development of plants because of its nutritional and physiological regulatory functions. In addition, nitrate can be used as the sole nitrogen source for tissue culture of most plant species. [0004] The concentration of nitrate in the medium is generally tens of millimolar. However, there are many reports in the literature that the amount of nitrate used in the medium is actually severely excessive, that is, most of the added nitrate is not utilized by plants. Therefore, it is particularly important to judge the utilization efficiency of nitrate in tissue culture seedlings. The judgment of nitrate utilization efficiency of tissue culture seed...

Claims

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Application Information

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IPC IPC(8): A01H4/00G06F17/11
CPCA01H4/001G06F17/11
Inventor 吴沿友张开艳吴沿胜饶森苏跃方蕾李环刘丛强王世杰
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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