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Diagnostic marker for malignant glioma

A technology of malignant glioma and diagnostic markers, which is applied in the field of a diagnostic marker of malignant glioma, and can solve problems such as unclear genetic characteristics

Inactive Publication Date: 2017-08-11
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finally, the neural subtype, whose genetic characteristics are not yet clear

Method used

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  • Diagnostic marker for malignant glioma
  • Diagnostic marker for malignant glioma
  • Diagnostic marker for malignant glioma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Expression of the NES gene is detected by various techniques known in the art.

Embodiment 2

[0048] The expression product of the NES gene (eg nestin protein) is detected by various techniques known in the art.

Embodiment 3

[0050] 1. Materials and methods

[0051] 1.1 Patient tissue samples

[0052] We collected fresh tissue samples from 8 pairs of surgically resected glioblastoma (GBM) and paired normal brain tissues according to the latest WHO classification; the samples were all from Ganzhou People's Hospital. All patients signed informed consent, and this operation was approved by the Ethics Committee of Ganzhou People's Hospital.

[0053] 1.2 Reagent preparation

[0054] UA buffer formula: Urea48.048g, Tris 1.2114g, add 100ml water, adjust pH=8.5

[0055] IAA: 0.005M IAA dissolved in UA (13.875mg IAA dissolved in 1.5ml UA)

[0056] 200mM DTT: 30.86mg DTT dissolved in 1ml 100mM NH 4 HCO 3 middle

[0057] ABC / Ammonium bicarbonate: 0.05M ABC dissolved in H 2 O (395.3mg dissolved in 100ml ddH 2 O)

[0058] 50mM NH 4 HCO 3 : 50ul 1M NH 4 HCO 3 (79.06mg dissolved in 1ml ddH 2 O) add 950ul ddH 2 o

[0059] SDT lysate formula: SDS 4g, DTT 1.54g, Tris 1.21g, HCl to adjust pH=7.6

[0...

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Abstract

The invention relates to a diagnostic marker for malignant glioma and belongs to the technical field of molecular biology. Particularly, the marker is an NES gene and an expression product thereof. The diagnostic marker has the advantages that comprehensive genomics and proteomics analysis is carried out on a GBM, and through gene expression analysis based on a gene chip and RNA sequencing and proteome analysis of LC / MS / MS, the mRNA level and protein level of the NES gene are regulated up. Through survival analysis on the NES, over-expression of the NES is related to poor prognosis of a GBM patient, so that the NES can be used as a GBM biomarker and a potential therapeutic target. The NES gene is used for encoding nestin protein, is mainly applied to nerve cell expression, and can be used as the GBM biomarker and the potential therapeutic target.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a diagnostic marker for malignant glioma. Background technique [0002] The initial designation of GBM as glioblastoma multiforme was an attempt to describe the striking differences among different cells within the tumor, which also reflected molecular heterogeneity. Long before genomic technologies were able to define the molecular characteristics of GBM, a simple clinical phenomenon reshaped the concept of GBM as a single disease. Clinically, most glioblastomas (approximately 90%) are primary, and only a few progress from low-grade gliomas (secondary GBM). Primary and secondary GBMs show completely different molecular features, for example, epidermal growth factor receptor (EGFR) and phosphatase tensin homologue (PTEN) are often abnormal in primary GBMs, while TP53 is abnormal in secondary GBMs. Abnormal in episodic GBMS. Although secondary GBMs are rare, accountin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/158G01N33/57407G01N33/57484G01N2800/52
Inventor 符达马雨水吕中伟卢改霞
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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