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Tissue culture method of primulina guigangensis

A technology of primula moss and tissue culture, applied in the field of bioengineering, can solve the problems that tissue culture research has not yet been reported, and achieve the effect of uniform reproduction, high rooting rate, and good growth of offspring

Active Publication Date: 2017-08-18
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the tissue culture method has been studied on other plants of the genus Primulus, but there is no relevant report on the tissue culture research of this species

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for tissue culture of Guigang Primula lettuce, comprising the steps of:

[0027] (1) Disinfection and sterilization of explants: Take the young leaves with petioles of Primula Guigang in spring, soak them in washing powder for 10 minutes, rinse them under running water for 10 minutes, and brush the leaves gently with a brush; On the workbench, use a cotton ball dipped in 70% alcohol to wipe the surface of the leaves, rinse with sterile distilled water for 3 times, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile distilled water for 3 times, and then treat with 0.1% mercuric chloride solution for 8 minutes , rinsed 3 times with sterile distilled water; during the sterilization process, shake gently to make alcohol or mercuric acid fully contact the leaves; the 0.1% mercuric acid solution is added with Tween-20, and every 100mL of 0.1% mercuric acid solution is added 2 drops of Tween-20;

[0028] (2) First-generation induction: cut off the petio...

Embodiment 2

[0034] A method for tissue culture of Guigang Primula lettuce, comprising the steps of:

[0035] (1) Disinfection and sterilization of explants: take the young leaves of Primula guigang with petioles in spring, soak them in washing powder for 15 minutes, rinse them under running water for 15 minutes, and brush the leaves gently with a brush; On the workbench, use a cotton ball dipped in 70% alcohol to wipe the surface of the leaves, rinse with sterile distilled water for 5 times, soak the leaves in 75% alcohol for 12 seconds, rinse with sterile distilled water for 5 times, and then treat with 0.1% mercuric chloride solution for 10 minutes , rinsed with sterile distilled water for 5 times; during the sterilization process, shake gently to make alcohol or mercuric acid fully contact the leaves; the 0.1% mercuric acid solution is added with Tween-20, and every 100mL of 0.1% mercuric acid solution is added 2 drops of Tween-20;

[0036](2) First-generation induction: cut off the p...

Embodiment 3

[0042] A method for tissue culture of Guigang Primula lettuce, comprising the steps of:

[0043] (1) Disinfection and sterilization of explants: Take the young leaves with petioles of Primula Guigang in spring, soak them in washing powder for 12 minutes, rinse them under running water for 12 minutes, and brush the leaves gently with a brush; On the workbench, first wipe the surface of the leaves with a cotton ball dipped in 70% alcohol, rinse 4 times with sterile distilled water, soak the leaves in 75% alcohol for 11 seconds, rinse 4 times with sterile distilled water, and then treat with 0.1% mercuric chloride solution for 9 minutes , rinsed 4 times with sterile distilled water; during the sterilization process, shake gently to make alcohol or mercuric acid fully contact the leaves; the 0.1% mercuric acid solution is added with Tween-20, and every 100mL of 0.1% mercuric acid solution is added 2 drops of Tween-20;

[0044] (2) First-generation induction: cut off the petioles ...

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Abstract

The invention belongs to the technical field of biological engineering, and particularly relates to a tissue culture method of primulina guigangensis. The tissue culture method of the primulina guigangensis comprises the following steps of disinfection of an explant, primary culture, multiplication culture of an adventitious bud, callus differentiation, sound seedling culture and rooting culture. The tissue culture method of the primulina guigangensis, which is provided by the invention, has the advantages that the posterity is favorable in growth vigor, the propagation speed is quick, the propagation coefficient is large, the posterity is propagated tidily and consistently and the rooting rate is high.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for tissue culture of Guigang primula moss. Background technique [0002] Primulina guigangensis is a perennial herbaceous plant of the genus Primulina guigangensis in the family Gesneriaceae. This species was first discovered in Zhangmu Town, Guigang City, Guangxi in 2011. Primula Guigang grows in limestone mountains at an altitude of 160 meters, and can adapt to the significant alternation of dry and wet seasons every year. Due to its harsh environmental requirements, its ecological niche is extremely narrow, and its population is gradually decreasing. Carrying out artificial breeding research is the basis for species protection. Guigang Primula can be propagated by seeds and cuttings, but seed propagation is often affected by the provenance and germination rate, and Guigang Primula has no elongated stems for cuttings, so leaf cuttings are the best...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 闫海霞何荆洲黄昌艳卜朝阳邓杰玲关世凯陶大燕
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI