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Chirita pinnatifida tissue culture method

A technique for cultivating lip stalks and tissues, which is applied in the field of bioengineering to achieve the effects of high rooting rate, high transplanting survival rate, and fast propagation speed

Inactive Publication Date: 2017-02-15
FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

P. pinnata can be propagated by seeds or leaf cuttings, and tissue culture technology has the advantages of fast propagation speed, large reproduction coefficient, uniform reproduction of offspring, and can maintain the excellent characteristics of the original variety without being restricted by seasons. It is widely used in various ornamental plants, but it has not been applied to the pinnatifida.

Method used

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  • Chirita pinnatifida tissue culture method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] A method for tissue culture of C. palnata, comprising the steps of:

[0022] (1) Disinfection and sterilization of explants: get the young and tender blades of C. pinnata in spring, soak them in 0.1% washing powder solution for 25 minutes, rinse them under running water for 30 minutes, and gently scrub the blades with a soft brush. Then transfer to the ultra-clean workbench for sterilization operation, first use cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse with sterile water twice, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water three times, Then treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, and finally treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, and shake gently during the mercuric chloride sterilization process to make the chlorine The mercury fully contacts the leaves; the leaves are cut into 2cm×2cm in size, and ...

Embodiment 2

[0028] A method for tissue culture of C. palnata, comprising the steps of:

[0029] (1) Disinfection and sterilization of the explants: get the young leaves of C. pinnata in spring, soak them in 0.5% washing powder solution for 30 minutes, rinse them under running water for 30 minutes, and gently brush the leaves with a soft brush. Transfer to the ultra-clean workbench for sterilization operation, first use a cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse with sterile water twice, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water for 3 times, and then Treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, and finally treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, shake gently during the mercuric chloride sterilization process to make mercuric chloride Full contact with the leaves; the leaves were cut into 2cm×2cm size, inserted into MS med...

Embodiment 3

[0035] A method for tissue culture of C. palnata, comprising the steps of:

[0036] (1) Disinfection and sterilization of the explants: get the young leaves of C. pinnata in spring, soak them in 0.2% washing powder solution for 28 minutes, rinse them under running water for 30 minutes, and gently scrub the leaves with a soft brush. Then transfer to the ultra-clean workbench for sterilization operation, first use cotton ball dipped in 75% alcohol to wipe the surface of the leaves, rinse with sterile water twice, soak the leaves in 75% alcohol for 10 seconds, rinse with sterile water three times, Then treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, and finally treat with 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 3 times, and shake gently during the mercuric chloride sterilization process to make the chlorine The mercury fully contacts the leaves; the leaves are cut into 2cm×2cm in size, and inocula...

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Abstract

The invention belongs to the technical field of bioengineering and particularly relates to a Chirita pinnatifida tissue culture method. The Chirita pinnatifida tissue culture method includes the steps of explant sterilization, adventitious bud induction, subculture, rooting culture and acclimatization and transplant. The Chirita pinnatifida tissue culture method has the advantages of good offspring growth vigor, a large number of adventitious buds, high reproduction speed, large reproduction coefficient, uniformity in propagated offspring, high rooting rate and high transplanting survival rate.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for tissue culture of cotyledon pinnata. Background technique [0002] Chirita pinnatifida (Hand.-Mazz.) B.L. Burtt is a perennial herb of Gesneriaceae. Distributed in Guangxi, northern Guangdong, southeastern Guizhou, southern Hunan, Jiangxi, western Fujian, southern and western Zhejiang. Born on rocks or beside streams in valleys and forests at an altitude of 600-1500 meters. The whole herb is available for medicinal purposes and is used for bruises and other diseases. In addition, C. pinnata also has a very high ornamental value and has a broad application prospect. [0003] It is very difficult to cultivate C. pinnata, and it has strict requirements on the growth environment. It needs high air humidity and high soil humidity, but at the same time, the root system has strict requirements on soil water vaporization and oxygen content; avoid direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 闫海霞张自斌卜朝阳何荆洲邓杰玲黄昌艳
Owner FLOWER RES INST GUANGXI ACADEMY OF AGRI SCI