Corychophramus violaceua embryoid and plant cultivation method

A cultivation method and technology of embryoid bodies, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., to achieve the effects of not easy to mutate, high yield, and speed up the cultivation process

Inactive Publication Date: 2017-08-18
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the embryoid bodies and regenerated plants obtained from the free microspore culture of Z.

Method used

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  • Corychophramus violaceua embryoid and plant cultivation method
  • Corychophramus violaceua embryoid and plant cultivation method
  • Corychophramus violaceua embryoid and plant cultivation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The seeds of Zhugecai were collected from wild open-pollinated plants on the campus of Southwest University in Beibei, Chongqing, and propagated and preserved by the Chongqing Rapeseed Engineering Technology Research Center. On September 20, 2010, seedlings were sown in Daejeon. On November 7, the seedlings were transplanted into the soil in the glass greenhouse of Southwest University. A total of 100 plants were transplanted. After survival, about 5g of compound fertilizer was applied to each litter, and watered as needed. The greenhouse light is natural light, and the temperature is not specially controlled. In February 2011, after flowering, samples were taken for microspore culture. In October, the microspore plants were transplanted to the test field. After flowering in the next year, the morphology and fruitiness were identified, and samples were taken to observe the pollen fertility and chromosome number.

[0036] Wherein, the microspore culture specifically incl...

Embodiment 2

[0039] The seeds of Zhugecai were collected from wild open-pollinated plants on the campus of Southwest University in Beibei, Chongqing, and propagated and preserved by the Chongqing Rapeseed Engineering Technology Research Center. On September 20, 2010, seedlings were sown in Daejeon. On November 7, the seedlings were transplanted into the soil in the glass greenhouse of Southwest University. A total of 100 plants were transplanted. After survival, about 5g of compound fertilizer was applied to each litter, and watered as needed. The greenhouse light is natural light, and the temperature is not specially controlled. In February 2011, after flowering, samples were taken for microspore culture. In October, the microspore plants were transplanted to the test field. After flowering in the next year, the morphology and fruitiness were identified, and samples were taken to observe the pollen fertility and chromosome number.

[0040] Wherein, the microspore culture specifically incl...

Embodiment 3

[0043] The seeds of Zhugecai were collected from wild open-pollinated plants on the campus of Southwest University in Beibei, Chongqing, and propagated and preserved by the Chongqing Rapeseed Engineering Technology Research Center. On September 20, 2010, seedlings were sown in Daejeon. On November 7, the seedlings were transplanted into the soil in the glass greenhouse of Southwest University. A total of 100 plants were transplanted. After survival, about 5g of compound fertilizer was applied to each litter, and watered as needed. The greenhouse light is natural light, and the temperature is not specially controlled. In February 2011, after flowering, samples were taken for microspore culture. In October, the microspore plants were transplanted to the test field. After flowering in the next year, the morphology and fruitiness were identified, and samples were taken to observe the pollen fertility and chromosome number.

[0044] Wherein, the microspore culture specifically incl...

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Abstract

The invention discloses a corychophramus violaceua embryoid and plant cultivation method. The method comprises the following steps of corychophramus violaceua planting, microspore culture, and plant regeneration and transplanting. In the microspore culture stage, the heat-induced culture time and the activated carbon concentration influence the yield of embryoids, wherein ratio (mg/mL) of the addition amount of the activated carbon to the massive volume of a microspore NLN-13 suspension is 1:4-3:4, and the heat-induced culture condition specifically includes that dark culture is carried out for 1-7 days at the temperature of 32-35 DEG C. The method can be used for rapidly obtaining a large number of corychophramus violaceua embryoids and genetically stable regenerated plants, and is beneficial for accelerating the process of breeding a new variety of corychophramus violaceua.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for cultivating embryoid bodies and plants of Zhuge kale. Background technique [0002] Zhuge vegetable [Orychophragmus violaceus (L.) O.E. Schulz] is a plant of the family Cruciferae (Cruciferae), mainly distributed in China, and has natural distribution or artificial introduction and cultivation in many areas. Zhuge Cai is often used as gardens, urban greening ground cover plants and flower beds, also known as "Er Yuelan (orchid)". In addition to its high ornamental value, Zhuge Cai may also be an excellent gene donor material for creating safflower sightseeing rapeseed varieties. When the rapeseed relative Brassica alboglabra is crossed with Zhuge Cai, the hybrids will have purple or pink flowers (Yin Jiaming et al., 1998). In addition, Zhuge Cai is a very promising vegetable and feed germplasm resource. [0003] Zhuge Cai is widely distributed in my co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 殷家明林呐唐章林李加纳
Owner SOUTHWEST UNIVERSITY
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