Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of preparation method of biological nanomaterial with magnetic and its application

A biological material and magnetic technology, applied in the preparation of biological nanomaterials, the application field of biological nanomaterials in the field of organic wastewater treatment, to achieve the effect of reusing, reducing concentration and increasing removal effect

Active Publication Date: 2020-06-23
HUNAN UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some patents and documents have studied the treatment of printing and dyeing wastewater and heavy metals by magnetically immobilized yeast, but there is a certain gap in the treatment of some refractory organochlorine pesticide wastewater

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of biological nanomaterial with magnetic and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Step 1): Preparation of Nano-Fe 3 o 4 -CS-ECH: Take 70ml of 0.13mol / L ferric chloride solution containing 0.13mol / L hydrochloric acid and add it to 50ml of 20g / L chitosan solution containing 1% hydrochloric acid, mechanically stir for 1h at room temperature, then add 30ml. .1mol / L of Na 2 SO 3 solution, when the color of the solution changes from yellow to red and then to yellow, then quickly add 40ml of 12% ammonia water, black flocs will be produced in the solution immediately, then add 4.4ml of epichlorohydrin, and stir for 3 hours in a 60-degree water bath. Wash three times with ultrapure water, three times with absolute ethanol, and dry in vacuum at 60°C for 12 hours.

[0024] Step 2): yeast embedding: under sterile conditions, 0.05g nano-Fe 3 o 4 -CS-ECH, 2ml 40g / L sodium alginate solution, 1ml 2.5×10 6 Mix cfu / ml Saccharomyces cerevisiae spore liquid, drop the mixed solution into 15ml 50g / L sterile calcium chloride solution with a sterile syringe, fix at ro...

Embodiment 2

[0028] Step 1): Preparation of Nano-Fe 3 o 4 -CS-ECH: Take 70ml of 0.13mol / L ferric chloride solution containing 0.13mol / L hydrochloric acid and add it to 50ml of 20g / L chitosan solution containing 1% hydrochloric acid, mechanically stir for 1h at room temperature, then add 30ml. .1mol / L of Na 2 SO 3 solution, when the color of the solution changes from yellow to red and then to yellow, then quickly add 40ml of 12% ammonia water, black flocs will be produced in the solution immediately, then add 4.4ml of epichlorohydrin, and stir for 3 hours in a 60-degree water bath. Wash three times with ultrapure water, three times with absolute ethanol, and dry in vacuum at 60°C for 12 hours.

[0029] Step 2): yeast embedding: under sterile conditions, 0.15g nano-Fe 3 o 4 -CS-ECH, 2ml 60g / L sodium alginate solution, 1ml 2.5×10 6Mix cfu / ml Saccharomyces cerevisiae spore liquid, drop the mixed solution into 15ml 50g / L sterile calcium chloride solution with a sterile syringe, fix at roo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a biological nanomaterial with magnetism, a preparation method and an application thereof. The biological nanomaterial adopts saccharomyces cerevisiae in saccharomyces as carriers, and is loaded with nano Fe3O4 and a crosslinking product of chitosan and epichlorohydrin; the surface of the saccharomyces cerevisiae is wrapped with calcium alginate. The method comprises the following steps: firstly preparing the nano Fe3O4 and the crosslinking product of chitosan and epichlorohydrin, then mixing with saccharomyces cerevisiae spore liquid and a calcium alginate solution, dripping the mixed solution into a calcium chloride solution dropwise, and immobilizing to obtain nano Fe3O4-saccharomyces cerevisiae-calcium alginate microspheres, and culturing, thus obtaining a biological material with the magnetism. The biological material with the magnetism has the magnetism of the Fe3O4 and the microbial performance of the saccharomyces cerevisiae, has the advantages of good removing effect, easiness in separation, no pollution and the like, and can be applied in treating atrazine in an aqueous solution.

Description

technical field [0001] The invention relates to the technical field of wastewater biological treatment in the field of environmental protection, in particular to a preparation method of a magnetic bionano material, and also relates to the application of the bionano material in the field of organic wastewater treatment. Background technique [0002] With the growing population, there is an increasing demand for synthetic pesticides such as insecticides and herbicides in agriculture. Relevant studies have shown that pesticides can play a significant role in increasing food production. If the use of pesticides and other chemicals is stopped, hundreds of millions of people around the world will face hunger. However, the extensive use of pesticides has caused great pollution to the soil and water environment due to their difficult to degrade in the natural environment. Some pesticides have been proven to be carcinogenic to the human body and interfere with the endocrine system. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C02F3/34C02F101/36C02F101/38
CPCC02F3/347C02F2101/306C02F2101/36C02F2101/38C02F2305/06
Inventor 杨春平朱灿耀何慧军钱慧魏塞楠曾光明刘海洋邬鑫余佳平
Owner HUNAN UNIV