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High-yield fermentation method of bacterial cellulose

A technology of bacterial cellulose and fermentation method, which is applied in the field of fermentation of high-yield bacterial cellulose, can solve the problem of low yield and achieve the effect of increasing dry weight

Active Publication Date: 2017-08-18
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a method that can significantly increase the yield of bacterial cellulose, so as to overcome the current situation of low yield in the fermentation and production process of bacterial cellulose

Method used

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  • High-yield fermentation method of bacterial cellulose

Examples

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Embodiment 1

[0025] 1. Activation of acetic acid bacteria strains: Streak inoculation of Gluconacetobacter hansenii stored in a 4°C refrigerator on a BSH plate, culture at 30°C for 5 days for strain activation, and repeat this process twice;

[0026] 2. Yeast strain activation: Saccharomyces cerevisiae stored in a refrigerator at 4°C was streaked and inoculated on a YPD plate, cultured at 30°C for 2-3 days for strain activation, and this process was repeated twice;

[0027] 3. Preparation of acetic acid bacteria seed liquid: pick two activated single colonies of Gluconacetobacter hansenii and put them in a 250ml Erlenmeyer flask containing 100ml of BSH liquid medium, under the conditions of 30°C and 150r / min Cultivate for 24 hours to prepare seed solution, and expand the prepared seed solution twice with 10% inoculation amount;

[0028] 4. Preparation of yeast seed liquid: Pick two single colonies of activated Saccharomyces cerevisiae and place them in a 250ml Erlenmeyer flask filled with ...

Embodiment 2

[0043] 1. Activation of acetic acid bacteria strains: Streak inoculation of Gluconacetobacter hansenii stored in a 4°C refrigerator on a BSH plate, culture at 30°C for 5 days for strain activation, and repeat this process twice;

[0044] 2. Yeast strain activation: Streak inoculation of Candida utilis stored in a refrigerator at 4°C on a YPD plate, culture at 30°C for 2-3 days for strain activation, and repeat this process twice;

[0045] 3. Preparation of acetic acid bacteria seed liquid: pick two activated single colonies of Gluconacetobacter hansenii and put them in a 250ml Erlenmeyer flask containing 100ml of BSH liquid medium, under the conditions of 30°C and 150r / min Cultivate for 24 hours to prepare seed solution, and expand the prepared seed solution twice with 10% inoculation amount;

[0046] 4. Preparation of yeast seed solution: pick two single colonies of activated Candida utilis into a 250ml Erlenmeyer flask containing 100ml YPD liquid medium, and cultivate them f...

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Abstract

The invention discloses a high-yield fermentation method of bacterial cellulose. The method comprises the steps of mixing gluconacetobacter hasenii with saccharomycete to obtain a mixture, inoculating the mixture to an improved BSH fermentation culture medium, and carrying out standing culture at 28-30 DEG C for 6-10 days so as to ferment and produce the bacterial cellulose, wherein the saccharomycete is saccharomyces cerevisiae or candida utilis. The gluconacetobacter hasenii and a certain amount of saccharomyces cerevisiae and candida utilis are mixed and inoculated into the improved BSH fermentation culture medium for culturing, so that the yield of the bacterial cellulose is remarkably higher than that of single-bacteria fermentation of the gluconacetobacter hasenii, and the dry weight of a cellulose membrane can be furthest increased by above 500%.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a fermentation method for high-yield bacterial cellulose. Background technique [0002] At the end of the 19th century, British scientist Brown first discovered bacterial cellulose during experiments. Bacterial cellulose, like plant cellulose, is a macromolecule formed by connecting glucose through β-1,4-glycosidic bonds, and compared with plant cellulose, bacterial cellulose has many unique properties. First of all, bacterial cellulose has a high purity and hardly contains impurities such as hemicellulose, lignin and pectin; secondly, bacterial cellulose has a high elastic modulus, which is generally several times or even ten times that of plant cellulose , the high elastic modulus endows bacterial cellulose with high tensile strength; bacterial cellulose has strong water absorption and water permeability, and can absorb 60-700 times its dry weight of...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/02C12R1/865C12R1/72
CPCC12P19/04C12P39/00
Inventor 王延斌熊强陈海超应晗笑
Owner NANJING UNIV OF TECH
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