Traditional Chinese medicine composition, and preparation method and applications thereof
A composition and technology of traditional Chinese medicine, applied in the field of traditional Chinese medicine composition and preparation thereof, can solve the problems of strong skin irritation, slow effect, not conforming to lifestyle and the like
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Embodiment 1
[0050] Weigh 1g each of Chuanxiong, Baishao, Angelica, Ginseng, Atractylodes macrocephala, Astragalus, Baituckahoe, Ophiopogon japonicus, Dendrobium, Zhilicorice, 0.5g ginger, wash two to three times, add deionized water, steam reflux extraction for 3-5h, Filter with two layers of 1200-mesh filter cloth, centrifuge the filtrate for 10-15 minutes, take the supernatant and filter it with a microfiltration membrane to prepare the Chinese medicine extract.
[0051] The prepared Chinese medicine extract was made into a sample and recorded as sample L, and the gene copy number of aquaporin AQP3 was detected by real-time fluorescent quantitative PCR method, and the gene copy number was 1.3 units.
[0052] HaCaT cells were seeded in 96-well plates and cultured in a 37°C incubator. After the cells adhered to the wall, the HaCaT cells were given the culture solution containing sample L and cultured for 42 hours. After 42 hours, the cell morphology was observed with an inverted microscope...
Embodiment 2
[0054] In parts by weight, 99.3 parts of sample L, 0.2 part of ecological nutrient solution, and 0.5 part of hyaluronic acid were taken, and mixed uniformly to prepare a traditional Chinese medicine composition.
[0055] A sample made of the prepared traditional Chinese medicine composition was recorded as sample M, and the gene copy number of aquaporin AQP3 was detected by real-time fluorescent quantitative PCR method, and the gene copy number was 1.26 units.
[0056] The sample M was added to the culture medium of HaCaT cells, cultured for 42 hours, and the cell morphology was observed with an inverted microscope after 42 hours, as shown in Figure 1(2), it can be seen that the morphology of HaCAT cells added to sample M did not change significantly after 42 hours.
Embodiment 3
[0058] In parts by weight, 99.8 parts of the sample L and 0.2 part of the ecological nutrient solution were taken, and mixed uniformly to obtain a traditional Chinese medicine composition.
[0059] A sample made of the prepared traditional Chinese medicine composition was recorded as sample N, and the gene copy number of aquaporin AQP3 was detected by real-time fluorescent quantitative PCR method, and the gene copy number was 1.71 units.
[0060] Sample N was added to the culture medium of HaCaT cells and cultured for 42 hours. After 42 hours, the cell morphology was observed with an inverted microscope. As shown in Figure 1 (3), it can be seen that the morphology of HaCAT cells added to sample N did not change significantly after 42 hours.
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