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Food composition and method of use

A composition and animal technology, applied in the field of invention, can solve the problems of reducing the effectiveness of microorganisms, etc.

Pending Publication Date: 2017-08-29
HILLS PET NUTRITION INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, adding live microorganisms directly to the diet is sometimes more difficult because food processing may reduce the effectiveness of the microorganisms

Method used

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  • Food composition and method of use
  • Food composition and method of use
  • Food composition and method of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081]The results in Example 1 show that quinoa can augment certain parameters of the symbiont. Dogs were fed either a control diet or one of six diets containing different types of grains as described in Table 1. Fecal samples were collected and analyzed for the percentage of Lactobacilli in the total microbiota, the percentage of Bifidobacteria in the total microbiota, the percentage of Clostridium in the total microbiota, and the ratio of Firmicutes to Bacteroidetes.

[0082] Total fecal DNA was extracted from frozen fecal samples by using the MOBIO POWERFECAL DNA kit. After total DNA extraction, 16s rRNA amplicons were developed from the samples by PCR using a primer set spanning the V3 and V5 (dog) hypervariable regions, and then qualitatively analyzed by the AGILENT 2100 Bioanalyzer. After amplicon quality was verified, exponential PCR was performed, followed by library quantification, normalization, and pooling of samples. The final pool of pooled samples was loaded i...

Embodiment 2

[0101] Studies in cats have shown that quinoa increases certain parameters of the symbiont. Cats were fed either a control diet or one of six diets containing different types of grains as described in Table 1. Fecal samples were collected and analyzed for the percentage of Lactobacilli in the total microbiota, the percentage of Bifidobacteria in the total microbiota, and the percentage of Clostridium in the total microbiota.

[0102] Total fecal DNA was extracted from frozen fecal samples by using the MOBIO POWERFECAL DNA kit. After total DNA extraction, 16s rRNA amplicons were developed from the samples by PCR using a primer set spanning the V3 and V4 (cat) hypervariable regions, and then qualitatively analyzed by the AGILENT 2100 Bioanalyzer. After amplicon quality was verified, exponential PCR was performed, followed by library quantification, normalization, and pooling of samples. The final pooled sample pool was loaded into the MISEQ v3 (for cat) sample loading cartridg...

Embodiment 3

[0116] Dogs were fed either a control diet or one of six diets containing different types of cereals at concentrations of 5%, 10% or 20% as described in Table 1 . Fecal samples were collected and analyzed for metabolites.

[0117] Such as figure 1 As shown, fecal samples from dogs fed quinoa or buckwheat diets contained significantly higher levels of amino acids and their related metabolites compared to the other diet groups in the control group, suggesting that quinoa and buckwheat may contain greater amounts of proteins and / or induce different protein metabolism in dogs.

[0118] As shown in Figure 2, dogs fed the quinoa diet had significantly increased levels of indoleacetic acid and catechol sulfate, as well as decreased levels of 3-indoxyl sulfate and 4-hydroxy Methyl benzoate levels. Buckwheat and amaranth appear to increase levels of catechol sulfate when given in high concentrations.

[0119] As shown in Figure 3, dogs fed the quinoa diet had significant changes in...

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Abstract

The current invention relates to relates to methods of improving commensals in an animal by feeding the animal with a diet including quinoa grain. The quinoa grain is in an amount effective to increase parameters for commensals such as the percentage of lactobacillus in total microbiota, the percentage of bifidobacteria in total microbiota, the percentage of clostridium in total microbiota, and the firmicutes to bacteroidetes ratio. The current invention also relates to pet food compositions that include effective amount of quinoa grain to increase the commensals parameters. In addition, the methods of making such a food compositions are also disclosed.

Description

Background technique [0001] As food science and animal health research develops, more and more evidence shows that certain microorganisms can provide beneficial effects to animals. In general, symbionts are microorganisms that provide health benefits to a host animal. Animals such as but not limited to dogs and cats harbor trillions of gut microbes in their digestive systems such as but not limited to the intestine and colon. Gut microbes (or collectively, the microbiota) include commensals that confer beneficial effects on animal health. [0002] It is often desirable to improve the symbiont in an animal, in some cases by providing the animal with a different diet. However, adding live microorganisms directly to the diet is sometimes more difficult because food processing may reduce the effectiveness of the microorganisms. Therefore, there is a need to prepare animal foods that improve the symbionts in the microbiota in animals. Contents of the invention [0003] The pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K40/20A23K40/30A23K10/30A23K50/40
CPCA23V2002/00A23K40/20A23K40/30A23K50/40A23K10/30A23V2200/3202A23K40/25A23K50/42
Inventor 丹尼斯·朱厄尔安贝尔·福利斯达亚卡尔·巴德里
Owner HILLS PET NUTRITION INC
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