30 results about "Phylum Bacteroidetes" patented technology

Methods and compositions are provided for treating weight related conditions and metabolic disorders by altering microbiota in a subject. One aspect provides methods and compositions to alter microbiota in a subject by administering to the subject a composition that includes a substantially purified microbiota from phyla such as Bacteroidetes, Proteobacteria, Firmicutes and Verrucomicrobia or orders such as Bacteroidales, Verrucomicrobiales, Clostridiales and Enterobacteriales or genera such as Alistipes, Clostridium, Escherichia, and Akkermansia. Another aspect includes a pharmaceutical composition for altering microbiota that includes a therapeutically effective amount of substantially purified microbiota and a pharmaceutically acceptable carrier. Yet another aspect includes methods for treating a disorder, such as obesity, in a subject in need of such treatment by changing relative abundance of microbiota in a gastrointestinal tract of the subject without or in addition to a surgical procedure.

The invention provides a method for achieving recycling of carbon dioxide through a microbial electro-synthesis system. According to the method for achieving recycling of the carbon dioxide through the microbial electro-synthesis system, a mixed anaerobe serves as a cathode, the mixed anaerobe comprises Proteobacteria, Firmicutes and Bacteroidetes. The invention further provides a microbial electro-synthesis system device. The microbial electro-synthesis system device comprises at least an anode and at least a cathode, wherein the mixed anaerobe serves as the cathode, the mixed anaerobe comprises Proteobacteria, Firmicutes and Bacteroidetes. The method for achieving recycling of the carbon dioxide through the microbial electro-synthesis system and the microbial electro-synthesis system device can achieve that the carbon dioxide is reduced into volatile fatty acid by taking use of common electrode materials, the cost is saved, and meanwhile, substances with high added values are generated by taking use of and domesticating an enriched mixed bacterial system, so that the good effects are achieved on the three aspects of the environment, the energy and the resources.

The invention provides a lactobacillus plantarum improving easily-fat body and offspring weight and application thereof, and is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.14570. The lactobacillus plantarum has excellent acid and bile salt resistance, bile salt hydrolase activity and intestinal colonization ability. Animal experiments and human food experiments show that the lactobacillus plantarum can control weight gain of pregnant women and offsprings caused by a high-fat and high-sugar diet by bile salt hydrolase activity and regulating intestinal flora, at the same time, the lactobacillus plantarum can regulate the intestinal flora, optimize intestinal flora structure, increase number of beneficial bacteria, and improve ratio of bacteroidetes and firmicutes, thereby improving the easily-fat body. The animal experiments and human food experiments show that the lactobacillus plantarum has clear efficacy and high safety, and can reverse the easily-fat body by fundamentally improving the intestinal flora of humans, has a long-lasting non-rebound effect, and has great potential for market development.

The invention discloses a pezicula ericae strain with a plant growth promoting effect and application thereof. The pezicula ericae functional strain 3-41 belongs to Dermateaceae in Bacteroidetes. Thetaxonomy name of the strain is pezicula ericae strain 3-41 (Pezicula ericae being equal to Cryptosporiopsis ericae). The strain is preserved in Guangdong province microbe bacterial preservation centeron June 1, 2017, and has the preservation code being GDMCC No.60283. The pezicula ericae functional strain 3-41 is obtained through being separated from wild blueberry root systems; after the strainis inoculated into the culture variety of the blueberry 'Lanyu' pot plant seedlings, the blueberry root systems can be infected; the strain mainly infects the skin and the cortical layer cells; the mode belongs to a typical endophytic fungi fixed planting mode. In addition, the blueberry potted plant inoculation tests show that the inoculation strain 3-41 obviously promotes the growth of the overground parts of the planted blueberries; the pure culture can be realized; the industrial production can be easily realized; good application prospects are realized.

The invention discloses a kit for predicting adolescent obesity in childhood and an application thereof. The invention provides an application of a substance used for detecting the abundance of target bacteria in intestinal flora of children to be detected in preparation of products for predicting simple obesity of children, and the target bacteria are any one of Firmicutes, Clostridia, Clostridiales, Clostridiaceae, Clostridium, Bacteroidetes, Bacteroidia, Bacteroidales, Bacteroidaceae, Bacteroides, Oscillospira, Coriobacteriaceae and Collinsella. Specific information is obtained from a simple obesity queue of children to better embody the intestinal flora features of obesity of children, and by monitoring the abundance change of the characteristic species, the development and change of the obesity state can be predicated; the obtained intestinal flora features of obesity of children are compared with the intestinal flora features obtained by researching other diseases to further predict the risk and trend of related obesity diseases.

The disclosure generally provides a method of balancing microbiome and / or improving nutrient absorption, treating gut inflammation, and / or balancing the level of Firmicutes and Bacteroidetes in the gut of a subject in need thereof, the method comprising consumption by, or administration to the subject of a composition comprising a nutrient in combination with a probiotic composition comprising (i) an isolated non-pathogenic fungal strain and (ii) an isolated non-pathogenic bacterial strain.

A formulation to increase the ratio of gastrointestinal microbiota in phylum Bacteroidetes to microbiota of Firmicutes phylum preferably includes about 20-60 mg / kg of body weight of fermentable fiber, about 10-30 mg / kg of body weight of beta glucan and about 20-60 mg / kg of body weight of blueberry extracts or any fruit or berry ingredient preparation containing similar phenolics. This formulation is preferably used to control body weight, body composition, and blood glucose regulation, preferably in humans, and is preferably administered orally, preferably twice per day.

The invention discloses Antarcticibacterium flavum JB01H24 and an application thereof. The Antarcticibacterium flavum JB01H24 belongs to the Flavobacteriaceae in the Bacteroidetes according to the taxonomy and is preserved in the Guangdong microbiological culture collection center (GDMCC) in floor 5th, building 59th, courtyard 100th, Xianlie middle road, Guangzhou city, Guangdong province on August 31, 2017. After the Antarcticibacterium flavum JB01H24 is cultured in a denitrification medium with an initial concentration of nitrate nitrogen of 14 mg / L under 180 rpm and 28 DEG C for 72h, the nitrate nitrogen removal rate is greater than 95% and thus the Antarcticibacterium flavum JB01H24 can be used for treating nitrogen oxides and has a great significance for the development of a denitrification technology.

The invention discloses a method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms. The method comprises the following steps: firstly domesticating active sludge by utilizing enzymic preparation, and then treating the high-salt phenolic wastewater by collectively utilizing the enzymic preparation. The enzymic preparation can accelerate the domestication speed of the active sludge and can improve the performance of the active sludge, thereby enhancing the efficacy of the microorganisms for treating the wastewater, and prolonging the service life of a membrane, specifically as follows: the tolerance degree of the active sludge to the salt is increased from 100g / L to 180g / L, the tolerance degree to the phenol is increasedfrom 2500 mg / L to 3500 mg / L, the relative abundance of the flora of actinobacteria and bacteroidetes in the active sludge is increased, the decomposition rate of the phenol in MBR is increased by 40 to 60 percent, and the membrane pollution period is prolonged by 8 to 10 days. Therefore, the micrococcus luteus enzymic preparation not only can be used for rapidly domesticating the active sludge, but also can be used for improving the activity of the functional flora in the wastewater biological treatment system, can prolong the service life of the membrane, and provides a safe, cheap and efficient method for promoting the popularization and application of the microorganism technique and MBR in treating complicated industrial wastewater.

The invention relates to an illness state evaluating system and reagent kit for systemic lupus erythematosus, and belongs to the technical field of biomedicine detection. An evaluating system is provided for conveniently and swiftly evaluating the illness state of the systemic lupus erythematosus. The evaluating system comprises the steps of calculating the ratio of proteobacteria / bacteroidetes (Proteobacteria / Bacteroidetes,P / B) of intestinal tracts of a patient, comparing the ratio with the reference value of healthy crowds (the medical reference value range set based on the early-stage research result is 0.13 to 0.63), when the ratio is higher than the reference value of the healthy crowds, performing effective assistance on SLE disease evaluation; and when the ratio is increased along with increase of an SLE disease activity index (SLE disease activity index, SLEDAI) mark, performing effective evaluation on SLE disease activity according to changes of the ratio. In addition, the invention further provides a corresponding reagent kit.

The invention discloses a mixed bean dietary fiber and an enzymic method preparation method and application thereof. The mixed bean dietary fiber is prepared from mixed bean cells without cell walls.Plant cell wall Viscozyme L is used for degrading cell walls of mixed beans, the mixed beans include other beans except for soybeans, such as kidney beans, chick peas, mung beans and red kidney beans.The preparation method saves energy and is environmentally friendly; compared with mixed bean starch, the fermentation speed of mixed bean dietary fiber is slower under the in-vitro fermentation condition, fermentation lasts longer, the amount of butyrate generated within 24 h is similar to that of mixed bean starch, the number of Firmicutes bacteria can be specifically increased, the number of bacteroidetes and fusobacteria bacteria is restrained, and the prebiotic function of preventing and treating various metabolism related diseases can be brought into play.

The invention relates to a method for fast and quantitatively measuring the microbial community composition in the fermentation process of baijiu. The method is characterized in that phylum specific primers are used in a PCR system so that special amplification of microbial communities of a certain specific phylum can be performed in the fermentation process of baijiu, quantitative amplification is performed through a qPCR system, and quantitative recognition is achieved. According to the category of known microorganisms, primers of the bacteroidetes, proteobacteria, firmicutes, synergistetes and actinobacteria are respectively designed, and the microstructure change of microbial communities can be fast inspected in the baijiu fermentation process in the level of the phylum on the basis of quantitative amplification of the phylum specific primers.

Provided are means, methods, and compositions effective for obesity treatment using probiotics such as bifidobacteria or lactobacilli. Also, a method of selecting probiotic bacteria having a body fat reducing effect, and a method of selecting responders to probiotic bacteria having an anti-obesity action are provided. Provided is a composition for reducing body fat, or the like, which contains Bifidobacterium and / or lactobacilli as an active ingredient, and is used for a pre-obesity group or an obesity disease including intestinal flora in which the ratio of the phylum Firmicutes to the phylum Bacteroidetes has a high value.

The current invention relates to relates to methods of improving commensals in an animal by feeding the animal with a diet including quinoa grain. The quinoa grain is in an amount effective to increase parameters for commensals such as the percentage of lactobacillus in total microbiota, the percentage of bifidobacteria in total microbiota, the percentage of clostridium in total microbiota, and the firmicutes to bacteroidetes ratio. The current invention also relates to pet food compositions that include effective amount of quinoa grain to increase the commensals parameters. In addition, the methods of making such a food compositions are also disclosed.

The present invention provides a prophylactic or therapeutic composition for a metabolic disorder, a composition for amplifying the amount of short-chain fatty acid in the intestine, or a composition for decreasing a ratio of bacteria belonging to the Firmicutes to bacteria belonging to the Bacteroidetes in the intestine, each containing an exopolysaccharide produced by Leuconostoc mesenteroides, and further, a food, a pharmaceutical product, a feed and the like containing the composition.

The invention discloses a kit for predicting adolescent obesity in childhood and an application thereof. The invention provides an application of a substance used for detecting the abundance of target bacteria in intestinal flora of children to be detected in preparation of products for predicting simple obesity of children, and the target bacteria are any one of Firmicutes, Clostridia, Clostridiales, Clostridiaceae, Clostridium, Bacteroidetes, Bacteroidia, Bacteroidales, Bacteroidaceae, Bacteroides, Oscillospira, Coriobacteriaceae and Collinsella. Specific information is obtained from a simple obesity queue of children to better embody the intestinal flora features of obesity of children, and by monitoring the abundance change of the characteristic species, the development and change of the obesity state can be predicated; the obtained intestinal flora features of obesity of children are compared with the intestinal flora features obtained by researching other diseases to further predict the risk and trend of related obesity diseases.

The invention belongs to the technical field of sewer greasy dirt treatment, and particularly relates to a compound bacterium preparation for removing sewer greasy dirt and a preparation method of the compound bacterium preparation. The compound bacterium preparation is prepared from the following raw materials in parts by weight: 30 to 40 parts of proteobacteria fermentation liquor, 20 to 30 parts of thick-walled mycophylum fermentation liquor, 5 to 12 parts of acidobacter fermentation liquor, 15 to 20 parts of bacteroides fermentation liquor, 10 to 16 parts of menosphaeria virens fermentation liquor, 15 to 25 parts of bacillus mycophylum fermentation liquor, 5 to 10 parts of deinococcus-thermophilic mycophylum fermentation liquor and 12 to 20 parts of cyanobacteria fermentation liquor. The concentration of the phylum patellae viable bacteria in the phylum patellae viable bacteria fermentation liquor is greater than 1 * 10 < 8 > / ml. The compound bacteria preparation can effectively digest and absorb oil stains generated in kitchens so as to remove the oil stains in sewers.

Treatment with catestatin changes the proportion of two major phyla Bacteroidetes and Firmicutes in the gut microbiota in an opposite manner observed in intestinal disorders like IBD, IBS or non-intestinal disorders like obesity. Specifically, administration of an effective amount of catestatin increased the relative percentage of Bacteroidetes and decreased the relative percentage of Firmicutes in the gut microbiota.

The invention discloses a nucleic acid detection kit and detection method for firmicutes and bacteroides. According to the nucleic acid detection kit and detection method of the invention, highly conserved regions of the firmicutes and bacteroides are taken as target regions, and the detection method capable of simultaneously detecting the firmicutes and bacteroides is established by designing and optimizing specific primers and probes. Compared with a traditional bacterial identification method, the method has the advantages of being high in specificity, high in sensitivity, convenient to operate, rapid and accurate in detection and the like; the method can be used for relative quantitative detection of the firmicutes and bacteroides of actual sample intestinal flora; the method can also be used for quantitative detection of the ratio of the firmicutes to the bacteroides; the method is helpful for research on obesity inflammation and type 2 diabetes mellitus, and has important significance for regulating intestinal flora and promoting intestinal health through research on the intestinal flora.

It is provided a yeast product for use as a prebiotic agent, and a composition comprising it. It is also provided the non-therapeutic use of the yeast product, and the composition comprising it, as a prebiotic agent. The yeast product, and the composition comprising it, stimulate the growth of bacteria of the Bacteroidetes phylum in the mammal gut microbiota.

The invention discloses application of Tibetan glossy mushroom to preparing preparations for regulating intestinal floras in patients suffering from type II diabetes mellitus, and relates to application of Tibetan glossy mushroom to regulating intestinal floras. By the aid of the application of the Tibetan glossy mushroom to preparing the preparations for regulating the intestinal floras in the patients suffering from the type II diabetes mellitus, the problem of injury on organisms due to existing medicines with effects of regulating intestinal tracts can be solved. The Tibetan glossy mushroom can be used for reducing the abundance of phylum firmicutes in the intestinal tracts of the patients suffering from the type II diabetes mellitus, improving the abundance of phylum bacteroidetes in the intestinal tracts of animals suffering from type II diabetes mellitus, improving the abundance of Alistipes in the intestinal tracts of the animals suffering from the type II diabetes mellitus, reducing the abundance of Blautia in the intestinal tracts of the animals suffering from the type II diabetes mellitus, reducing the abundance of bacteroides in the intestinal tracts of the animals suffering form the type II diabetes mellitus, improving the abundance of lactobacillus in the intestinal tracts of the animals suffering from the type II diabetes mellitus and improving the diversity of intestinal floras in the animals suffering from the type II diabetes mellitus. The application has the advantages that the Tibetan glossy mushroom can be used for regulating the intestinal floras in the animals suffering from the type II diabetes mellitus, obvious regulation effects can be realized, and the Tibetan glossy mushroom is free of optional injury on organisms.

The invention discloses a method for treating high-salt phenolic wastewater by utilizing micrococcus luteus enzymic preparation to enhance microorganisms. The method comprises the following steps: firstly domesticating active sludge by utilizing enzymic preparation, and then treating the high-salt phenolic wastewater by collectively utilizing the enzymic preparation. The enzymic preparation can accelerate the domestication speed of the active sludge and can improve the performance of the active sludge, thereby enhancing the efficacy of the microorganisms for treating the wastewater, and prolonging the service life of a membrane, specifically as follows: the tolerance degree of the active sludge to the salt is increased from 100g / L to 180g / L, the tolerance degree to the phenol is increasedfrom 2500 mg / L to 3500 mg / L, the relative abundance of the flora of actinobacteria and bacteroidetes in the active sludge is increased, the decomposition rate of the phenol in MBR is increased by 40 to 60 percent, and the membrane pollution period is prolonged by 8 to 10 days. Therefore, the micrococcus luteus enzymic preparation not only can be used for rapidly domesticating the active sludge, but also can be used for improving the activity of the functional flora in the wastewater biological treatment system, can prolong the service life of the membrane, and provides a safe, cheap and efficient method for promoting the popularization and application of the microorganism technique and MBR in treating complicated industrial wastewater.

The invention discloses a fluorescent quantitative PCR detection kit and a method thereof for predicting sepsis infection. The kit includes: Bacteroidetes forward primer and reverse primer, Firmicutes forward primer and reverse primer, Proteobacteria forward primer and reverse primer, Actinobacteria forward primer and reverse primer Primers, PCR master mixes and reagents for fluorescent quantitative PCR detection methods. The invention provides reliable evidence of infection and the etiological type of infection for the diagnosis of sepsis by detecting the classification level structure of human intestinal flora, can be used for auxiliary diagnosis of clinical sepsis, and provides relevant antibiotic treatment for patients. Effective basis, good prospects for clinical application.

The invention discloses a method for controlling membrane pollution in HMBR. The method is to control membrane pollution in HMBR by cultivating dominant microbial populations in activated sludge, wherein the dominant microbial populations include Proteobacteria, Acidobacteria, and Bacteroides phyla, and the ratios of Proteobacteria, Acidobacteria, and Bacteroides in the total bacterial population gradually decreased. The invention controls the membrane fouling in the HMBR by cultivating the dominant microbial population in the activated sludge, the method is simple, does not increase the operating cost, and at the same time can make the operating period of the membrane module the longest, that is, the membrane fouling degree is the lightest, and the effect is remarkable .

The present invention relates to methods and compositions for modulating gastrointestinal microflora of a canine. In one embodiment, a method of modulating gastrointestinal microflora in a canine cancomprise administering to the canine a pet food composition comprising from about 25% to about 60% protein, from about 5% to about 30% carbohydrates, fat, and fiber; where after administration, the Bacteroidetes to Firmicutes (B / F) ratio of the canine is less than 0.8.

The invention provides a method for separating and identifying cellulose-degrading bacteria in the intestinal tract of monochamus monochamus larvae, belonging to the technical field of microbes. Grind the hindgut of Monochamus alternatus larvae to obtain the original bacterial liquid, which is then serially diluted and spread on No. 4 medium with sodium carboxymethyl cellulose as the only carbon source for aerobic culture , Obtained single colony for morphological observation and molecular identification. According to the method provided by the present invention, 154 strains of bacteria with cellulase activity are isolated from the middle hindgut of Monochamus alternata larvae, belonging to 8 genera of Proteobacteria, Firmicutes and Bacteroidetes respectively, 10 strains. The separation and identification method provides a theoretical basis for revealing the interaction relationship between intestinal bacteria and Monochamus alternatus.

The invention discloses a preparation method of a soil conditioner applicable to harmful microorganisms. The preparation method comprises the following steps: uniformly mixing 50 to 70 parts of modified biological charcoal, 10 to 20 parts of a fermentation solution and 1 to 3 parts of waterborne acrylic resin and drying to obtain the soil conditioner. The preparation method disclosed by the invention has the beneficial effects that all components in the conditioner have a mutual effect and the variety of bacteria in treated soil is relatively abundant; the relative abundance of acidobacteria is remarkably increased and the relative abundance of proteobacteria is reduced; the relative abundance of GAL15 and AD-3 is remarkably reduced and the relative abundance of chloroflexi, verrucomicrobia and gemmatimonadetes is remarkably increased; the relative abundance of planctomycetaceae is remarkably increased and the relative abundance of bacteroidetes is remarkably increased; the micro-ecology of the soil is improved and the occurrence probability of root diseases of plants is reduced.

The current invention relates to relates to methods of improving commensals in an animal by feeding the animal with a diet including quinoa grain. The quinoa grain is in an amount effective to increase parameters for commensals such as the percentage of lactobacillus in total microbiota, the percentage of bifidobacteria in total microbiota, the percentage of clostridium in total microbiota, and the firmicutes to bacteroidetes ratio. The current invention also relates to pet food compositions that include effective amount of quinoa grain to increase the commensals parameters. In addition, the methods of making such a food compositions are also disclosed.