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Biological preparation for controlling porcine diarrhea and preparation method of biological preparation

A technology for biological preparations and pig diarrhea, applied in the field of biological preparations, can solve the problems of soil and water environment pollution, piglet growth performance decline, public health and safety problems, etc., to improve economic benefits, reduce piglet mortality, and reduce morbidity. Effect

Inactive Publication Date: 2017-09-01
黑龙江省兽医科学研究所
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Problems solved by technology

Piglet diarrhea is one of the important causes of piglet growth performance decline and death, which has caused major economic losses to the pig industry. At present, antibiotics are widely used in clinical prevention and treatment of piglet diarrhea, resulting in antibiotics and drug residues, causing many food safety hazards
In addition, the manure of piglets with diarrhea contains a large number of pathogenic microorganisms, which will cause environmental pollution such as soil and water, and lead to a series of public health and safety problems

Method used

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  • Biological preparation for controlling porcine diarrhea and preparation method of biological preparation
  • Biological preparation for controlling porcine diarrhea and preparation method of biological preparation
  • Biological preparation for controlling porcine diarrhea and preparation method of biological preparation

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specific Embodiment approach 1

[0029] The specific embodiment one, a kind of preparation method of preventing and treating porcine diarrhea biological preparation in the present embodiment is characterized in that this diarrhea biological preparation is carried out according to the following steps:

[0030]Step a. Inoculate the SY1-Lactobacillus acidophilus strain into the MRS liquid medium, place it in anaerobic culture at 37°C for 36-48 hours, complete the activation, and then streak inoculate it on the MRSA plate, select more than 5 typical colonies, and inoculate Several branches were planted on MRSA slant, cultured at 37°C for 348 hours, and used as primary seeds. The primary seeds were inoculated in MRS liquid medium, and cultured at 37°C for 48 hours, which were secondary seeds. The secondary seeds of Lactobacillus acidophilus are inoculated into the fermenter for submerged liquid culture. The liquid medium is MRS, the inoculum size is 5%, the liquid volume is 80%, and the culture is static under the ...

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Abstract

The invention discloses a biological preparation for controlling porcine diarrhea and a preparation method of the biological preparation, and belongs to the field of biological preparations. The biological preparation for controlling porcine diarrhea, disclosed by the invention, is prepared by the steps: activating lactobacillus acidophilus, lactobacillus plantarum, bacillus subtilis and bacillus amyloliquefaciens respectively and sequentially, performing secondary seed culture, performing fermentation cultivation, concentrating by centrifuging, performing spray drying, and mixing. The biological preparation for controlling porcine diarrhea, disclosed by the invention, has the advantages of being high in specificity, high in viable count, green, safe, zero in toxic or side effects and the like. The biological preparation for controlling porcine diarrhea can achieve the effects of effectively controlling diarrhea of piglets, reducing the dosage of antibiotics and improving the mean daily gain of the piglets and the feed conversion ratio. The biological preparation is applicable to popularization and application in large-scale farms, particularly green ecological pig rearing communities, so that healthy farming is realized, and requirements on green pigs are met. Rapid development of the large-scale live pig raising industry in China can be greatly promoted, and the biological preparation has significances on income increase of farmers and rural economic development in China.

Description

technical field [0001] The invention belongs to the field of biological preparations; in particular, it relates to a biological preparation for preventing and treating swine diarrhea and a preparation method thereof. Background technique [0002] In recent years, my country's pig industry has developed rapidly, and the number of large-scale and intensive pig farms is increasing day by day. While the number of live pigs is increasing, the hazards caused by many diseases have attracted people's attention. Piglet diarrhea is one of the important causes of piglet growth performance decline and death, which has caused major economic losses to the pig industry. At present, antibiotics are widely used in clinical prevention and treatment of piglet diarrhea, resulting in antibiotics and drug residues, causing many food safety hazards . In addition, the manure of piglets with diarrhea contains a large number of pathogenic microorganisms, which will cause environmental pollution such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P1/12A23K50/30A23K10/18A61K35/742
Inventor 李阳王爽刘宇张鹏宇陈亮刘海瑞刘洪贵石跃李丹刘梦迪兰世捷苗艳吴宪金振华
Owner 黑龙江省兽医科学研究所
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