Data acquisition device and excitation state service life measurement method based on excitation state service life measurement of frequency count card

A technology of frequency counting and data acquisition, used in instruments and other directions

Inactive Publication Date: 2017-09-01
大连创锐光谱科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the limitations of the existing excited state lifetime

Method used

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  • Data acquisition device and excitation state service life measurement method based on excitation state service life measurement of frequency count card
  • Data acquisition device and excitation state service life measurement method based on excitation state service life measurement of frequency count card
  • Data acquisition device and excitation state service life measurement method based on excitation state service life measurement of frequency count card

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Experimental program
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Embodiment 1

[0025] Embodiment 1: a kind of data acquisition device based on the excited state lifetime measurement of frequency counting card, described device comprises pulse excitation light source 1, sample cell 2, fluorescence collection system 4, the light-emitting port of described excitation light source 1 and fluorescence collection system Facing the sample cell, in order to obtain the time interval between the photon pulse signal and the excitation light pulse signal, the device also includes a data acquisition system, the data acquisition system includes a frequency counter card, which is connected to the signal synchronous output of the pulse excitation light source 1 pin, and is connected with the signal output pin of the photodetector of the light collector 3.

[0026] The device also includes a light collector, and the light emitting port of the excitation light source 1 is in a straight line with the center of the sample pool 2 and the light collector 3 . The purpose is to ...

Embodiment 2

[0036] Embodiment 2: The purpose of this embodiment is to provide a method and device for measuring excited state lifetimes that are compatible with a wide range of lifetimes (such as hundreds of picoseconds to milliseconds) and are cheaper, that is, they can be used to measure short-lived fluorescence samples, and also for long-lived phosphorescent samples.

[0037] The excited state lifetime measurement method based on the frequency counting card includes the following steps:

[0038] 1) Excite the sample to be tested with pulsed light at a high repetition rate to generate photoluminescence;

[0039] 2) Detecting the photoluminescent signal of the sample, using a frequency counting card to measure the time interval (time difference) between the detected photon pulse signal and the excitation light pulse signal;

[0040] 3) dividing the measured photon time difference into various time intervals, and assigning photons with different time difference data into corresponding ti...

Embodiment 3

[0054] Example 3: For the investigation of the excited state lifetime measuring device described in the present invention when measuring short-lived samples, No. 1 short-lived sample (its fluorescence peak position is about 610nm) was used for testing. First, put the cuvette containing sample No. 1 into the sample cell, use picosecond pulse laser (405nm) to excite the sample, and set the laser repetition frequency to 20MHz; use a 405nm notch filter, and a fluorescence filter of 610nm Narrow-band filter, light-receiving and converging lens all adopt 60mm focal length lens, the measured fluorescence decay curve of this sample is as follows figure 2 shown. Using a single exponential decay function I(t)=I 0 exp(-t / τ)+c (where τ is the fluorescence lifetime of the sample to be tested, and c is the background noise) performs data fitting on the decay curve, and it can be obtained that the fluorescence lifetime of the sample to be tested is about τ=6.37ns .

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Abstract

A data acquisition device for measuring excited state lifetime based on a frequency counting card and a method for measuring excited state lifetime, the measuring method includes the following steps: exciting the sample to be tested with a high repetition frequency pulsed laser to generate photoluminescence, and detecting the photoluminescence of the sample Light-emitting signal, use the frequency counting card to measure the time interval between the detected photon pulse signal and the excitation light pulse signal, divide the measured photon time difference into various time intervals, and distribute the photons with different time difference data into the corresponding In the time zone; count the photons in each time zone separately to obtain the photoluminescence photon luminous intensity decay curve, and perform exponential decay fitting on the luminous intensity curve to obtain the excited state lifetime of the sample to be tested. Compared with other fluorescence or phosphorescence lifetime measurement methods, the method of the invention can measure a wider range of excited state lifetime data and lower cost; meanwhile, it also has high time resolution and detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of excited state lifetime measurement, and in particular relates to an excited state lifetime measurement method and device based on a frequency counting card. Background technique [0002] In the field of scientific research, the measurement of the excited state lifetime of samples is one of the important means to study the material structure and physical and chemical properties of various molecules or materials; especially in the fields of life science and material science, the fluorescence lifetime of the samples studied Measurement is the most routine detection method in scientific research in this field. At present, there are several methods for measuring the excited state lifetime (including fluorescence or phosphorescence lifetime) of samples: 1) Time-correlated single photon counting (TCSPC), combined with a special TCSPC card and a highly sensitive photodetector, can be used to measure Fluorescence...

Claims

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Application Information

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IPC IPC(8): G01J11/00
CPCG01J11/00
Inventor 金盛烨
Owner 大连创锐光谱科技有限公司
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