Bursin extraction method

A bursalin and extraction method technology, applied in the biological field, can solve the problems of unstable conformation, difficult to obtain double-stranded DNA, easy aggregation, etc., and achieve the advantages of promoting the increase of animal body weight, improving the feed conversion rate, and improving the effect of vaccines. Effect

Inactive Publication Date: 2017-09-05
派生特(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it is chemically synthesized or cloned, the development and application of BS peptide drugs have certain limitations.
First of all, the conformation is unstable, and natural linear peptides have good biological activity and stability in vitro, but the conformational changes caused by the molecular flexibility of linear peptides affect the strength and selectivity of binding to receptors; secondly, Low bioavailability, poor membrane permeability, easy to be degraded by enzymes, easy to remove, difficult to dissolve and easy to aggregate in some cases, make it difficult for peptides...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for extracting bursalin, comprising the following steps:

[0040] 1) Take the chicken bursa, remove fat and fascia, wash it with water for injection several times, mix it evenly according to the weight ratio of bursa and water for injection at 1:2, and then use a tissue masher in turn according to the speed of 168008r / min , 188008 r / min, 21500r / min to mash to obtain a mash liquid;

[0041] 2) The above crushed liquid is homogenized by a colloid mill to obtain a homogenized liquid;

[0042]3) Freeze and thaw the above homogenate repeatedly 4 times, then centrifuge the frozen and thawed homogenate at 7000 rpm for 25 min at 2°C to obtain the primary supernatant and precipitate, and store the primary supernatant at -20°C save;

[0043] Add an equal volume of water for injection with a pH value of 3.0 to the precipitate, and homogenize it through a colloid mill to obtain a second homogenate. The second homogenate is repeatedly frozen and thawed twice, and then the...

Embodiment 2

[0050] A method for extracting bursalin, comprising the following steps:

[0051] 1) Take the chicken bursa, remove fat and fascia, wash it with water for injection several times, mix it evenly according to the weight ratio of bursa and water for injection at 1:3, and then use a tissue masher in turn at a speed of 17000r / min , 19000 r / min, 22000r / min to mash to obtain a mash liquid;

[0052] 2) The above crushed liquid is homogenized by a colloid mill to obtain a homogenized liquid;

[0053] 3) Freeze and thaw the above homogenate repeatedly 6 times, then centrifuge the freeze-thawed homogenate at 4°C and 8000 rpm for 20 minutes to obtain the primary supernatant and precipitate, and store the primary supernatant at -20°C;

[0054] Add an equal volume of water for injection with a pH value of 3.5 to the precipitate, and homogenize through a colloid mill to obtain a secondary homogenate, freeze and thaw the secondary homogenate repeatedly for 3 times, and then freeze and thaw t...

Embodiment 3

[0061] A method for extracting bursalin, comprising the following steps:

[0062] 1) Take the chicken bursa, remove fat and fascia, wash it with water for injection several times, mix it evenly according to the weight ratio of bursa and water for injection at 1:1, and then use a tissue masher in turn at a speed of 16000r / min , 18000 r / min, 21000 r / min to mash to obtain a mash liquid;

[0063] 2) The above crushed liquid is homogenized by a colloid mill to obtain a homogenized liquid;

[0064] 3) The above homogenate was repeatedly frozen and thawed 6 times, and then the homogenate after freezing and thawing was centrifuged at 6000 rpm for 30 min at 6°C to obtain the primary supernatant and precipitate, and the primary supernatant was stored at -20°C save;

[0065] Add an equal volume of water for injection with a pH value of 2.5 to the precipitate, homogenize through a colloid mill to obtain a secondary homogenate, freeze and thaw the secondary homogenate repeatedly 4 times,...

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PUM

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Abstract

The invention discloses a bursin extraction method. The method includes: well mixing pretreated chicken bursa fabricius with water for injection, mashing and homogenizing; subjecting the homogenized bursa fabricius to twice extraction to obtain extract liquid, and adding beta-propiolactone for inactivation; hydrolyzing inactivated extract liquid to obtain hydrolysate; adopting a 0.22-0.45micron filter membrane for tangential flow microfiltration of the hydrolysate, collecting microfiltration permeation liquid, adopting a 1-5kD filter membrane for tangential flow ultrafiltration, and collecting ultrafiltration permeation liquid; adjusting an osmotic pressure of the ultrafiltration permeation liquid to obtain isoosmotic solution, adopting 0.1-0.22micron sterilization filter for sterilization, and collecting liquid namely bursin. The bursin extraction method is simple and convenient, the bursa fabricius utilization rate is increased, production cost is reduced, production time is shortened, and the extracted bursin can serve as an immunity enhancer, a feed additive, an auxiliary therapeutic agent and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting bursalin. Background technique [0002] Bursa of fabricus (BF) is the unique central humoral immune organ of poultry, which is equivalent to the bone marrow of mammals. There are many biologically active substances in BF, especially some small peptides with immunoregulatory effects, which can promote the differentiation and development of lymphocytes and the maturation of immune organs in birds and mammals. Generally, this type of small molecule bioactive peptides is called Farrell. Bursin (bursin, BS). In recent years, people have conducted extensive research on the biological function and mechanism of BS. It has been confirmed that most of BS have immunoregulatory functions, such as bursin tripeptide, the amino acid composition sequence is Lys-His-Gly-NH2, in which Lys:His:Gly:NH2 is 1:0.9:1:0.8, which can induce mammalian And the differentiat...

Claims

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Application Information

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IPC IPC(8): C07K14/465C07K1/14C07K1/34
CPCC07K14/465
Inventor 吕小婷胡万荣徐磊刘友霖
Owner 派生特(福州)生物科技有限公司
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