Primary culture method for salivary gland cells of Hirudo nipponia
A Japanese leech, primary culture technology, applied in the field of bioengineering, can solve the problems of low anticoagulant activity, low hirudin yield, high cost and high cost, achieve high anticoagulant activity, protect animal germplasm resources, and improve yield. Effect
Active Publication Date: 2017-09-05
CHINA JILIANG UNIV
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[0003] In summary, the existing problems in the prior art are: the current method for obtaining hiru
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[0018] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0019] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.
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The invention discloses a primary culture method for salivary gland cells of Hirudo nipponia, belonging to the field of bioengineering technology. The method comprises the following steps: inoculating an enclosed cell culture flask having a volume of 25 cm<2> with scissored tissue blocks; adding 5 mL of a SFX-INSCET insect cell culture solution; and placing the flask in a biochemical incubator for static culture at a temperature of 29 DEG C. The method provided by the invention substantially increases the output of natural hirudin; and a great number of salivary gland cells can be obtained after in-vitro cell culture of a tiny number of salivary gland cells of wild Hirudo nipponia, and a great amount of natural hirudin is separated and extracted, so wild Hirudo nipponia populations are protected, deep development and utilization are realized, and the germplasm resources of animals are substantially protected. The method is rapid, effective and repeatable, improves culture of leech cells and provides reliable test data and foundation for research on primary culture of leech cells.
Description
technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for primary culture of Japanese hirudo salivary gland cells. Background technique [0002] With the rapid development of life sciences, cell culture is an essential process for both the entire bioengineering technology and one of the biological cloning technologies, and promotes the development of cell biology, molecular biology, biochemistry development in other fields. Japanese medical leeches (Hirudo nipponia) belong to the phylum Annelids, which include the subphylum Annulus, class Hirudo, order Hirudo nipponia, family Hirudo, and genus Hirudo. According to statistics, there are more than 600 species of leeches distributed all over the world, and nearly 100 species are distributed in my country. They live in rivers, wet land, and fields. Some live by feeding on the blood of mammals such as fish, frogs, water birds, cattle, horses, and humans, and...
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Login to view more IPC IPC(8): C12N5/07A01K67/033
CPCA01K67/033C12N5/0601
Inventor 邢月婷罗媛媛管峰王为民
Owner CHINA JILIANG UNIV
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