Method of establishing DNA molecular label of Yunrui-series maize cultivar

A technology of DNA molecules and DNA fingerprints, which is applied in the field of molecular biology, can solve problems such as variety management troubles, damage to the interests of the country and farmers, and combating original breeding, etc., to achieve easy observation, easy band statistics, and suppression of non-specific amplification. Effect

Inactive Publication Date: 2017-09-19
INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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Problems solved by technology

[0003] With the continuous improvement of our country's breeding level, new bred varieties are constantly emerging, but the phenomenon of "variety homogeneity" is also becoming more and more serious. Phenomena such as "same origin but different names" and "d

Method used

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  • Method of establishing DNA molecular label of Yunrui-series maize cultivar
  • Method of establishing DNA molecular label of Yunrui-series maize cultivar
  • Method of establishing DNA molecular label of Yunrui-series maize cultivar

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example 1

[0037] Example 1 The method of establishing DNA molecular tags of Yunrui series corn variety Yunrui 508 (Dian Shenmaize No. 2015036)

[0038] (1) Genomic DNA extraction of Yunrui series corn varieties:

[0039] Take about 200-300mg of Yunrui 508 leaves, put them in a 2.0mL centrifuge tube, add liquid nitrogen and grind them thoroughly. Add 700 μL of CTAB extract preheated at 65°C to each tube, mix well, bathe in water at 65°C for 60 min and mix by inverting for 2-3 times. Add an equal volume of a mixture of chloroform and isoamyl alcohol to each tube, the volume ratio of chloroform: isoamyl alcohol is 24:1, mix well, let stand for 10 minutes, and centrifuge at 12,000 rpm for 15 minutes. Transfer the supernatant to a new tube, add an equal volume of isopropanol pre-cooled at 0°C, mix by inversion, place at -20°C for 30 minutes, then centrifuge at 4°C and 12,000 rpm for 10 minutes, discard the supernatant, and add 70% v / v ethanol, discard the ethanol solution after rotating 2...

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Abstract

The invention provides a method of establishing a DNA molecular label of a Yunrui-series maize cultivar. The method is applied to cultivar molecular identification and cultivar management. The method is characterized by screening and differentiating 4 pairs of core primers of the Yunrui-series maize cultivar and optimizing a PCR reaction system, carrying out digital coding assignment of cultivar DNA information, forming a two-dimensional code representation, and marking the same on a commodity seed package for using for anti-counterfeiting and tracing of seeds of the fine maize cultivar.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to the establishment of a DNA molecular tag of Yunrui series corn varieties, which is applied to variety molecular identification and variety management. Background technique [0002] Yunnan belongs to the southwest and belongs to the mountainous corn planting area in southwest my country. Although Yunnan Province is not the main corn producing province in my country, it has a unique natural environment and climate resources, which provide more possibilities for the selection of corn germplasm resources. Yunrui series new varieties (lines) are a series of the most representative corn varieties (lines) in Yunnan Province selected and bred by the Yunnan Academy of Agricultural Sciences. Good and other characteristics, social and economic benefits are remarkable. This series of varieties has been promoted to more than 40 million mu in Yunnan, Sichuan, Guizhou, Gu...

Claims

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Application Information

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IPC IPC(8): G06F19/16
CPCG16B15/00
Inventor 张建华张鹏管俊娇殷长生黄清梅刘艳芳王江民杨晓洪毛进马芙蓉李彦刚
Owner INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
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