A kind of water extract of plantain algae and its application

A technology of water extract, Oscillation algae, applied in the application, food extraction, drug combination and other directions, can solve the problems such as no reports related to the application of Oscillation algae, achieve significant antioxidant effect, reduce the level of ROS in the body, improve effect on survival

Active Publication Date: 2019-12-13
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no report on the application of Pseudomonas

Method used

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  • A kind of water extract of plantain algae and its application
  • A kind of water extract of plantain algae and its application
  • A kind of water extract of plantain algae and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 The preparation of the water extract of Pleurotus algae

[0035] S1. Take the fresh wet algae, freeze at -20°C for 12 hours, thaw at room temperature for 10 hours, repeat freezing and thawing 5 times, centrifuge at 8000r / min for 10min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0036] S2. To the precipitation described in step S1, according to the solid-to-liquid ratio of wet algae and ethanol 1g / mL, add ethanol with a volume fraction of 80% for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. Centrifuge for 30 minutes at a speed of 8000 r / min for 15 minutes, separate the supernatant from the precipitate, and take the precipitate for later use;

[0037] S3. Wash the precipitate described in step S2 twice with primary water, centrifuge for 15 minutes at a speed of 8000r / min, separate the supernatant from the precipitate, re...

Embodiment 2

[0039] Example 2 The preparation of the water extract of Pleurotus algae

[0040] S1. Take fresh wet algae, freeze at -30°C for 12 hours, thaw at room temperature for 12 hours, repeat freezing and thawing 4 times, centrifuge at a speed of 10,000r / min for 5min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0041] S2. Add ethanol with a volume fraction of 90% to the precipitation described in step S1 according to the solid-to-liquid ratio of wet algae and ethanol of 1 g / mL for ultrasonic extraction 3 times, the frequency of ultrasonic extraction is 35 kHz, and the temperature is 4 ° C. The time is 20min, centrifuged at 10000r / min for 10min, the supernatant and the precipitate are separated, and the precipitate is taken for later use;

[0042]S3. Wash the precipitate described in step S2 with primary water for 3 times, centrifuge for 15 min at a speed of 10000r / min, separate the supernatant from the pre...

Embodiment 3

[0043] Example 3 The preparation of the water extract of Pleurotus algae

[0044] S1. Take fresh wet algae, freeze at -40°C for 10 hours, thaw at room temperature for 12 hours, freeze and thaw repeatedly 3 times, centrifuge at 5000r / min for 15min, and remove the supernatant The solution and the precipitate were separated, and the precipitate was taken for later use;

[0045] S2. Add ethanol with a volume fraction of 70% to the precipitation described in step S1 according to the solid-to-liquid ratio of wet algae and ethanol of 1g / mL for ultrasonic extraction 4 times, the frequency of ultrasonic extraction is 40kHz, and the temperature is 4°C. The time is 20min, centrifuged at 5000r / min for 15min, the supernatant and the precipitate are separated, and the precipitate is taken for later use;

[0046] S3. Wash the precipitate described in step S2 twice with primary water, centrifuge for 15 minutes at a speed of 10000r / min, separate the supernatant from the precipitate, remove th...

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Abstract

The invention belongs to the technical field of marine organisms and provides a Planktothrix. water extract and application thereof. The Planktothrix. water extract is characterized in that fresh wet Planktothrix. is subjected to multigelation, centrifugation is performed, and the supernatant is removed; the sediment is taken and added with ethyl alcohol, ultrasonic extraction is performed, centrifugation is conducted, and the supernatant is removed; and the sediment is taken and washed with water, water is added, and heating extraction is conducted to prepare the Planktothrix. water extract. The provided Planktothrix. water extract can relieve premature senility caused by oxidative stress, promote elimination of in-vivo reactive oxide species (ROS), and reduce the level of the in-vivo reactive oxide species (ROS), has a remarkable anti-oxidation function, and can be used for preparing health food and medicines with the antioxidant function.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a water extract of plantain algae and an application thereof. Background technique [0002] Microalgae are a group of single-celled microalgae with heterogeneous physiology and biomorphology, and there are more than 200,000 species on the earth. Microalgae produce many bioactive substances, most of which are used in pharmaceutical production, food or feed additives, cosmetics, and other applications. [0003] Active oxygen, such as superoxide anion, hydroxyl radical, hydrogen peroxide radical, etc., will attack lipids, proteins, enzymes, DNA, RNA and other biologically active molecules, causing cell or tissue damage, aging and even cancer. Antioxidants can effectively remove active oxygen and protect the body from damage by active oxygen. As a class of seaweed with important research and utilization value, microalgae has always been one of the research hots...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/748A23L33/00A61P39/06
CPCA23L33/00A23V2002/00A61K35/748A23V2200/30A23V2300/14A23V2300/48A23V2300/20
Inventor 胡丽莉黄泽波杨子寒宋立荣余程明
Owner GUANGDONG PHARMA UNIV
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