Method for evaluating detection quality of pesticide residue quick detection instrument

A technology for pesticide residues and quality inspection, applied in measuring devices, color/spectral characteristic measurements, instruments, etc., can solve problems such as missing additions, out-of-control inspection quality, and limit the development of the pesticide residue inspection industry, so as to achieve improved capabilities and improved management horizontal effect

Inactive Publication Date: 2017-09-26
GUANGZHOU RUISEN BIOTECH
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  • Abstract
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Problems solved by technology

[0003] Although there are currently many manufacturers of rapid pesticide residue detectors, none of them can evaluate the detection quality of the rapid pesticide residue detectors. The supervision of detection quality has been out of control for a long time: (1) In order to obtain good detection results, There are cases of falsification, such as using "water" instead of "extract of the sample to be tested", and it is impossible to truthfully report the real pesticide residues of the sample to be tested; (2) The test operator is perfunctory or makes mistakes, omits or adds wrong reagents, As a result, the real pesticide residues of the samples to be tested cannot be reported truthfully; (3) Whether the enzyme reagent in the test is invalid; etc.
This often leads to completely different test results of the same sample in different testing agencies, which leads to flaws in the public's trust in testing agencies, and also limits the development of the pesticide residue testing industry.

Method used

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  • Method for evaluating detection quality of pesticide residue quick detection instrument
  • Method for evaluating detection quality of pesticide residue quick detection instrument
  • Method for evaluating detection quality of pesticide residue quick detection instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for evaluating the detection quality of a pesticide residue rapid detector, including the following steps:

[0044] A. Obtain standard reference data of different vegetable varieties:

[0045] A1. For the same vegetable variety, use at least three samples from different sources to prepare sample extracts: extract samples with buffer to obtain sample extracts;

[0046] A2. Using at least one calibrated rapid pesticide residue detector, repeat the test for different sample extracts of each vegetable variety to obtain the initial absorbance value, and calculate the average value and standard deviation of the initial absorbance of each vegetable variety extract 1. Take the average value of the initial absorbance as the initial absorbance reference value X1 of each vegetable variety extract;

[0047] A3. Put the different sample extracts of each vegetable variety into the detection channel of the calibrated rapid pesticide residue detector, add enzyme and color reagent to e...

Embodiment 2

[0080] The calibrated rapid pesticide residue detector used in Example 1 can be any one in the prior art, or the rapid pesticide residue detector disclosed in Example 2.

[0081] The enzyme inhibition method is based on the principle of insect toxicology that organophosphorus and carbamate pesticides can inhibit the activity of acetylcholine in the central and peripheral nervous systems of insects, resulting in the accumulation of acetylcholine, a nerve conduction medium, affecting normal nerve conduction, and causing insects to be poisoned and killed. Detected. According to this principle, by reacting specifically inhibiting cholinesterase (ChE) with the sample extract, if ChE is inhibited, it indicates that the sample extract contains organophosphorus or carbamate pesticides (the rapid pesticide residue detector uses This method).

[0082] Such as Figure 1~2 As shown, a rapid pesticide residue detector includes a sample tank, a control circuit, a computer / tablet computer, a mi...

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Abstract

The invention relates to the technical field of food safety, and discloses a method for evaluating detection quality of a pesticide residue quick detection instrument. The method comprises the following steps of obtaining standard reference data of different vegetable varieties in advance; when an operator detects a to-be-detected vegetable sample, comparing the measured initial absorbance value of the to-be-detected vegetable sample extracting liquid, the stable absorbance value after reaction for 10min by adding enzyme and a coloring agent, and the standard reference data of the vegetable variety; comparing the linear correlation coefficient Y3 of absorbance value and time of the to-be-detected sample extracting liquid and the preset value, and evaluating whether the operation of an operator is standard or not, so as to judge whether the obtained enzyme inhibiting rate value after detection is valid or not. The evaluation method has the advantages that the ability of base level detection controlling the pesticide residue risk is improved, and the management level of the pesticide residue detection is improved.

Description

Technical field [0001] The invention relates to the technical field of food safety, in particular to a method for evaluating the detection quality of a pesticide residue rapid detector. Background technique [0002] As food safety is getting more and more attention, the state and social institutions have invested a lot of money to purchase various rapid testing equipment to analyze pesticide residues. Among them, enzyme inhibition spectrophotometry is a more commonly used method for rapid detection of pesticide residues, which is based on changes in absorbance. [0003] Although there are many manufacturers of various pesticide residue fast detectors, none of them can evaluate the detection quality of pesticide residue fast detectors. The supervision of detection quality has been out of control for a long time: (1) In order to obtain good detection results, There are cases of falsification, such as "water" instead of "test sample extract", and it is impossible to truthfully report...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/314G01N2021/3181
Inventor 梁伟能
Owner GUANGZHOU RUISEN BIOTECH
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